LOCUS       sequence-288603-        7712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of 5BoxB-7-Fluc-CG11206.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Eulalio A, Huntzinger E, Nishihara T, Rehwinkel J, Fauser M, 
            Izaurralde E
  TITLE     Deadenylation is a widespread effect of miRNA regulation.
  JOURNAL   RNA. 2009 Jan . 15(1):21-32.
  PUBMED    19029310
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jan . 15(1):21-32."
COMMENT     Sequence Label: pAc5.1C-5BoxB-7-Fluc-CG11206_D
FEATURES             Location/Qualifiers
     source          1..7712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2490..2508
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2532..2550
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2556..2574
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2614..2632
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2638..2656
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             2795..4450
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MAEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
                     PANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQ
                     SMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSH
                     ARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDY
                     KIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQG
                     YGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIM
                     SGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESI
                     LLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGV
                     VFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2864..2882)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     rep_origin      complement(5895..6483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5975..5994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6654..7445,7446..7514))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7277..7296
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7515..7619)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcaagcttg ggccctgaag aagggcccct cgactagtcc
     2521 aaatacaaac tgggccctga agaagggccc atatagggcc ctgaagaagg gccctatcga
     2581 ggatattatc tcgactaagt ccaactacaa actgggccct gaagaagggc ccatataggg
     2641 ccctgaagaa gggccctatc gaggatatta tctcgaggtc acccattcga agcttcgttt
     2701 gagttcttgt gctgtgtgga tactcctccc gacacaaagc cgctccatca gccagcagtc
     2761 gtctaatcca gagaccccgg atcggggtac caacatggcc gaagacgcca aaaacataaa
     2821 gaaaggcccg gcaccattct atccactaga ggatggaacc gctggagagc aactgcataa
     2881 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     2941 ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa
     3001 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     3061 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     3121 ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt
     3181 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa
     3241 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     3301 cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg
     3361 tgacaaaaca attgcactga taatgaactc ctctggatct actgggttac ctaagggtgt
     3421 ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg
     3481 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     3541 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     3601 tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt
     3661 accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     3721 tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc
     3781 aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc
     3841 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     3901 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg
     3961 cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac
     4021 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     4081 agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca aagggtatca
     4141 ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg
     4201 cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     4261 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     4321 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     4381 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc
     4441 caaattgtaa gaattcgagt gaaaagctag ctagatagga ttacaactac cactaccaaa
     4501 gatactcagc tgtactcccg catgaaaatc cctttcaccc ccccataacc catttaccct
     4561 ttcagctgca tttgagctta gaatctacaa gcaaactcaa ttatattcaa gtcagtgtca
     4621 gacccaccta caagatacaa gcattcagca gctacagata catatgcgtc aagtaataca
     4681 agataagcga gtaatcattt gatcaaatca gatagaaatt cgaactatat gctaaacata
     4741 aataacgaca taattatatt gtgtagacta agccccaatc gcaggcaaga gaagtaagga
     4801 tcgaatcaac tcgaatcaat tcaaatcgaa tcaaaaactc caccccaact ccaacaaact
     4861 gcattagtct aatcaatcac atatatgtaa ttctaattgt aattgtaact gtaaagatag
     4921 ctctaaaccg atttaaaagt gagaggcaga ttgcgtgacg gtaatatact cactgaatta
     4981 caagccgatt tgggcactgg agatgcgatt gcaatttaga taaattgaaa ttattttgta
     5041 accgaaacta tcgtagctaa gcaaatggaa acggaaacta ttgaattaaa tttgataagc
     5101 cgcaagatgc ggcactttgt aaattatgat aagccctaag caacgttcaa tatcgcatgg
     5161 taatggtaaa atccgaaaca gaatcaaata tatggtaaat acaaacgcac tccccaaaaa
     5221 aaaccctcgc acaaccaaag aaagtcagtc agcctgcttt atccgccaat caatcagtat
     5281 cgaagaatcc ccgagagcgt cagggtatca ttaagctgac caatttaact gtagacctat
     5341 gtcgaagaaa actatcgaat aaacccatga tcaaaactct gtcgaccgat gcccttgaga
     5401 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     5461 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     5521 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     5581 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     5641 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     5701 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     5761 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     5821 cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc
     5881 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5941 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6001 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6061 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     6121 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6181 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6241 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6301 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6361 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6421 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6481 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6541 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6601 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6661 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6721 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6781 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6841 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6901 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6961 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7021 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7081 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7141 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7201 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7261 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7321 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7381 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7441 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7501 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7561 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7621 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7681 tataaaaata ggcgtatcac gagccctttc gt
//
