LOCUS       sequence-288610-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmUPF1-V5His6_E
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      4807..4816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6899..6940
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6950..6967
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6990..7007)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7040..7174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7064..7083)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7118..7137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7796..8384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7876..7895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8555..9346,9347..9415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9178..9197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9416..9520)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9588..9606
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcat gagcgtggac acgtacgcgc ccagctcggc
     3361 gctctcgttc ctggacatgg acgacaacga gctgcttccg ggagcggata ctcaacccac
     3421 gcagtacgat taccgcgact ttaccatgcc ctccacctcg cagagccaga cccagaacga
     3481 tcagctggag attgcccaac gctgctctgc cggagactcg catccacgac tggccagcat
     3541 caccaacgat ctggccgatc tgcagttcga agaggaggac gacgagcctg gcagctcgta
     3601 tgtgaaggag ctgccgccgc atgcgtgcaa gtattgcggc atccatgatc cagccacggt
     3661 ggtcatgtgc aacaactgcc gcaaatggtt ttgcaacggt cgtggaagca cttccggttc
     3721 gcacatcatc aaccatctgg tgagggccaa gcatcgcgag gtgacgctcc acggggaagg
     3781 tcccctgggc gagacaatcc tggagtgcta ctcctgtggt gtgcgcaacg tctttgtgct
     3841 gggcttcatt ccggccaagg ccgattctgt ggtcgtgctg ctctgccgtc agccgtgtgc
     3901 cgcccagaat tcgctaaagg atatgaactg ggaccaggaa cagtggaagc ctctaattgc
     3961 agaccgctgc tttttggcct ggctggtaaa gcaacccagc gaacagggac agctgcgagc
     4021 tcgccaaatc tcagccgctc agatcaacaa gctggaggag ctatggaagg agaatattga
     4081 ggccacgttt caggatctgg agaagccagg cattgactcg gagccagcac atgtgctact
     4141 ccgctacgag gatggctatc agtacgagaa gacctttggg ccgctggtcc gccttgaggc
     4201 cgaatacgac caaaaactga aggagtctgc cacgcaggag aacatcgaag tacgctggga
     4261 cgtcggcctc aacaaaaaga ccattgccta ctttacgctg gcgaagaccg attcggacat
     4321 gaagctcatg catggcgacg agctgcgcct gcattatgtg ggcgagctgt acaatccgtg
     4381 gagcgagatc ggccacgtta tcaaggtgcc ggacaatttc ggcgatgacg tcggcctgga
     4441 gctgaaatcc tcaacgaatg ccccggttaa gtgcaccagt aactttacgg tggacttcat
     4501 ctggaagtgc acgtcatttg atcgcatgac acgtgctctg tgcaaattcg ccatcgatcg
     4561 caattcagtg tcgaacttca tctactcgcg cctgttgggc cacggtcgtg cggattccaa
     4621 cgacgaggtg ctgttccgcg gcccacaacc caagctcttc agtgccccgc atctgccgga
     4681 tttgaatcgc agccaggtgt atgccgtgaa acacgcgctt cagcgtccgc tctcgctaat
     4741 ccaagggccg cctggcacgg gcaaaaccgt gacctcggcg accatcgttt accagctggt
     4801 caagctccat ggtggcacag tgctggtgtg cgctcccagc aacacggccg tggatcagct
     4861 aactgagaag atccaccgaa caaaccttaa agtggtgcgt gtttgcgcca agagccgtgg
     4921 ggccatcgat agcccggtaa gcttcctggc gctgcacaac caaatccgta acatggagac
     4981 caactcggag ctaaagaagc tgcagcagct gaaagacgag accggcgagc tgagctcagc
     5041 agacgaaaag cgataccgca acctgaaacg tgccgccgag aaccaactgc tggaggctgc
     5101 cgacgttatc tgctgcacat gcgtaggcgc cggcgatggt cgtctatcgc gagtcaagtt
     5161 cacctcgatc ctgatcgatg agtctatgca gtcgacggag ccggagtgca tggtgccagt
     5221 ggtgctgggc gctaagcagc tgatcctcgt gggcgatcac tgccagctgg gaccggttgt
     5281 tatgtgcaag aaagcagctc gtgccggcct ctcgcaaagt ttgttcgagc gcctggtggt
     5341 tctgggcatc cgtccgttcc ggctggaggt gcaatatcgc atgcaccccg agctgtccca
     5401 gttcccgtcc aacttcttct acgagggatc gctgcaaaac ggcgtctgcg cggaggatcg
     5461 tcgccttaag cttgatttcc cctggccaca gccggagaga ccgatgttct ttctggtaac
     5521 acagggacag gaggagattg ccggctccgg cacctctttc ctcaaccgca cagaggcggc
     5581 caacgtggaa aagattacga cgcgattcct taaggcaggc atcaagccgg aacaaattgg
     5641 aatcatcacg ccttacgaag gtcagcgcgc ttacctggtg cagtacatgc aataccaggg
     5701 cagcctgcac tctcgtctat accaggagat cgagatcgcc agtgtggacg cgttccaggg
     5761 acgtgagaag gacatcatta tcatgtcttg cgtgcgatct aacgaacgtc aaggcatcgg
     5821 ctttttgaac gacccacgtc gccttaatgt tgcccttaca cgggccaagt tcgggatcat
     5881 cattgtgggc aatcccaaag tgctcgccaa gcagcagctg tggaaccatc tgctcaactt
     5941 ctacaaggac cgtaaggtgc tcgtcgaggg atctctgaat aaccttaagg agtcgctaat
     6001 ccacttccag aagcccaaaa agcttgtcaa cagcatgaac attggggcac actttatgtc
     6061 caccattatt gccgatgcca aggaagtgat ggtgccaggc tccatttacg accgcagtgg
     6121 cggttacggc caaggtcgcc aaatggtggg acagtcaatg aatggcggac agtacggtgg
     6181 cagtggaggt ggtccctacg gaaactcacc cctcggctac ggtactccca gctccaattc
     6241 catggtgggc tttggcctgg gcaacggagg caatggcgcg gccggtggca acaacaactt
     6301 cggaggggct gaacccagtt gggcggctgc ccacctccac cacgactcca ttggctatat
     6361 atccaacgag catggagcag cagcactggg caacatgcca gttccggttg gcatgttcat
     6421 gaacatgagc aatattccgc cgcgtttcta caaccagcac cagcaggcga tcatggcggt
     6481 caagcagaat cgcgccattc aacaacagac gggtaatttc tctcccggta actcgggtcc
     6541 tggagtcact ggagtcggag tcggacgaag cgccacccca ggcggcaata agaagaccaa
     6601 caagctggga aaatcgcgcg taacgggcgg tggaactggc ggggcaccgc taacacaagg
     6661 aagctcggta tgcaatgctg ctccatacag tcagcacccg atgcctttgt cgctgcagat
     6721 gacccagccc agcggatttg ctctgtccca gcagccggaa ctttcacagg actttgggca
     6781 aatatcgcag atggacggtt tgctatccca ggatgttgcc tttaacgcgt cgggcgagcg
     6841 gagcttgaat cagttctcac agccttatcg ctcgagtcta gagggcccgc ggttcgaagg
     6901 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     6961 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg
     7021 ctgatcagcc tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac
     7081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     7261 tggtatggct gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct
     7321 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     7381 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     7441 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     7501 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     7561 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     7621 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     7681 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     7741 ctcttaccag ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7981 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8221 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     8881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9121 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     9181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9421 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9541 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9601 cgaggccctt tcgt
//
