LOCUS       sequence-28862-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  TITLE     Ghosh Lab Plasmids
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Myc-G4-ND2 (N67)
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        232..250
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     232..249
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     263..282
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             376..405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
       61 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
      121 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
      181 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
      241 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
      301 ctgcacctcg gttctatcga ttgaattccc gaaccgacag tcggtctctt caccaaggcc
      361 attcgcgcca ccatggagca aaagctcatt tctgaggaag atctcaatgg tggtggtggt
      421 gggtcgatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
      481 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
      541 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
      601 tcaaggctag aaagactgga acagctattt ctactgattt ttccctcgag
//
