LOCUS       sequence-28867-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Du K, Asahara H, Jhala US, Wagner BL, Montminy M
  TITLE     Characterization of a CREB gain-of-function mutant with constitutive
            transcriptional activity in vivo.
  JOURNAL   Mol Cell Biol. 2000 Jun . 20(12):4320-7.
  PUBMED    10825195
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Jun . 20(12):4320-7."
COMMENT     Sequence Label: pCF CREB M1
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             276..299
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctc cacaattcgg gcggcggctg ctgctgcctg tggcccgggc
      121 ggctgggaga agccgagtgt tggtgagtga cgcggcggag gtgtagtttg acgcggtgtg
      181 ttacgtgggg gagagaataa aactccagcg agatccgggc cgcgaacgaa agcagtgacg
      241 gaggagcttg taccaccggt gactagatga ccatggacta caaggacgac gatgacaagc
      301 tgcaggaatt cggatccgac tctggagcag acaaccagca gagtggagat gctgctgtaa
      361 cagaagctga aagtcaacaa atgacagttc aagcccagcc acagattgcc acattagccc
      421 aggtatccat gccagcagct catgcgacgt catctgctcc cactgtaacc ttagtgcagc
      481 tgcccaatgg gcagacagtc caggtccatg gggtcatcca ggcggcccag ccatcagtta
      541 ttcagtctcc acaagtccaa acagttcagt cttcctgtaa ggacttaaaa agacttttct
      601 ccggaactca gatttcaact attgcagaaa gtga
//
