LOCUS       sequence-288671-        8063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsAGO2-F505V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Eulalio A, Huntzinger E, Izaurralde E
  TITLE     GW182 interaction with Argonaute is essential for miRNA-mediated 
            translational repression and mRNA decay.
  JOURNAL   Nat Struct Mol Biol. 2008 Apr . 15(4):346-53.
  PUBMED    18345015
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Apr . 15(4):346-53."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsAGO2-F505V_E
FEATURES             Location/Qualifiers
     source          1..8063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4658..4667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5348..5389
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5399..5416
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5439..5456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5489..5623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5513..5532)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5567..5586
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6245..6833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6325..6344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7004..7795,7796..7864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7627..7646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7865..7969)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8037..8055
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgggagg gcacccattc cagtggtgta acgcacttgc
     2761 acctcctgcg ccgccgcccc ccatccaagg atatgccttc aagcctccac ctagacccga
     2821 ctttgggacc tccgggagaa caatcaaatt acaggccaat ttcttcgaaa tggacatccc
     2881 caaaattgac atctatcatt atgaattgga tatcaagcca gagaagtgcc cgaggagagt
     2941 taacagggaa atcgtggaac acatggtcca gcactttaaa acacagatct ttggggatcg
     3001 gaagcccgtg tttgacggca ggaagaatct atacacagcc atgccccttc cgattgggag
     3061 ggacaaggtg gagctggagg tcacgctgcc aggagaaggc aaggatcgca tcttcaaggt
     3121 gtccatcaag tgggtgtcct gcgtgagctt gcaggcgtta cacgatgcac tttcagggcg
     3181 gctgcccagc gtcccttttg agacgatcca ggccctggac gtggtcatga ggcacttgcc
     3241 atccatgagg tacacccccg tgggccgctc cttcttcacc gcgtccgaag gctgctctaa
     3301 ccctcttggc gggggccgag aagtgtggtt tggcttccat cagtccgtcc ggccttctct
     3361 ctggaaaatg atgctgaata ttgatgtgtc agcaacagcg ttttacaagg cacagccagt
     3421 aatcgagttt gtttgtgaag ttttggattt taaaagtatt gaagaacaac aaaaacctct
     3481 gacagattcc caaagggtaa agtttaccaa agaaattaaa ggtctaaagg tggagataac
     3541 gcactgtggg cagatgaaga ggaagtaccg tgtctgcaat gtgacccggc ggcccgccag
     3601 tcaccaaaca ttcccgctgc agcaggagag cgggcagacg gtggagtgca cggtggccca
     3661 gtatttcaag gacaggcaca agttggttct gcgctacccc cacctcccat gtttacaagt
     3721 cggacaggag cagaaacaca cctaccttcc cctggaggtc tgtaacattg tggcaggaca
     3781 aagatgtatt aaaaaattaa cggacaatca gacctcaacc atgatcagag cgactgctag
     3841 gtcggcgccc gatcggcaag aagagattag caaattgatg cgaagtgcaa gtttcaacac
     3901 agatccatac gtccgtgaat ttggaatcat ggtcaaagat gagatgacag acgtgactgg
     3961 gcgggtgctg cagccgccct ccatcctcta cgggggcagg aataaagcta ttgcgacccc
     4021 tgtccagggc gtctgggaca tgcggaacaa gcagttccac acgggcatcg agatcaaggt
     4081 gtgggccatt gcgtgcttcg ccccccagcg ccagtgcacg gaagtccatc tgaagtcctt
     4141 cacagagcag ctcagaaaga tctcgagaga cgccggcatg cccatccagg gccagccgtg
     4201 cttctgcaaa tacgcgcagg gggcggacag cgtggagccc atggtccggc acctgaagaa
     4261 cacgtatgcg ggcctgcagc tggtggtggt catcctgccc ggcaagacgc ccgtgtacgc
     4321 cgaggtcaag cgcgtgggag acacggtgct ggggatggcc acgcagtgcg tgcagatgaa
     4381 gaacgtgcag aggaccacgc cacagaccct gtccaacctc tgcctgaaga tcaacgtcaa
     4441 gctgggaggc gtgaacaaca tcctgctgcc ccagggcagg ccgccggtgt tccagcagcc
     4501 cgtcatcttt ctgggagcag acgtcactca cccccccgcc ggggatggga agaagccctc
     4561 cattgccgcc gtggtgggca gcatggacgc ccaccccaat cgctactgcg ccaccgtgcg
     4621 cgtgcagcag caccggcagg agatcataca agacctggcc gccatggtcc gcgagctcct
     4681 catccagttc tacaagtcca cgcgcttcaa gcccacccgc atcatcttct accgcgacgg
     4741 tgtctctgaa ggccagttcc agcaggttct ccaccacgag ttgctggcca tccgtgaggc
     4801 ctgtatcaag ctagaaaaag actaccagcc cgggatcacc ttcatcgtgg tgcagaagag
     4861 gcaccacacc cggctcttct gcactgacaa gaacgagcgg gttgggaaaa gtggaaacat
     4921 tccagcaggc acgactgtgg acacgaaaat cacccacccc accgagttcg acttctacct
     4981 gtgtagtcac gctggcatcc aggggacaag caggccttcg cactatcacg tcctctggga
     5041 cgacaatcgt ttctcctctg atgagctgca gatcctaacc taccagctgt gtcacaccta
     5101 cgtgcgctgc acacgctccg tgtccatccc agcgccagca tactacgctc acctggtggc
     5161 cttccgggcc aggtaccacc tggtggataa ggaacatgac agtgctgaag gaagccatac
     5221 ctctgggcag agtaacgggc gagaccacca agcactggcc aaggcggtcc aggttcacca
     5281 agacactctg cgcaccatgt actttgcttg aggcggccgc tcgagtctag agggcccgcg
     5341 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     5401 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     5461 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     5521 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5581 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5641 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     5701 ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc
     5761 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     5821 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     5881 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     5941 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     6001 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     6061 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     6121 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     6181 cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6421 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6661 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     7321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7621 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8041 ggcgtatcac gaggcccttt cgt
//
