LOCUS       sequence-288674-        9362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delRBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Eulalio A, Tritschler F, Buttner R, Weichenrieder O, Izaurralde E, 
            Truffault V
  TITLE     The RRM domain in GW182 proteins contributes to miRNA-mediated gene 
            silencing.
  JOURNAL   Nucleic Acids Res. 2009 May;37(9):2974-83. doi: 10.1093/nar/gkp173. 
            Epub 2009 Mar 18.
  PUBMED    19295135
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkp173"; journalName: "Nucleic Acids Res"; date: 
            "2009-05"; volume: "37"; issue: "9"; pages: "2974-83"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delRBD_E
FEATURES             Location/Qualifiers
     source          1..9362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6647..6688
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6698..6715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6738..6755)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6788..6922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6812..6831)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6866..6885
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7544..8132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7624..7643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8303..9094,9095..9163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8926..8945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9164..9268)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9336..9354
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggaggta acgggtcaag caatataact ggatcgtctg
     3181 gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg taacggttgg ggagaccctc
     3241 gtgaaatacg ccctttggga gttggtggct ccatggatat tcgaaatgtt gaacatcgcg
     3301 gcggtaacgg ttctggagca acttcgagcg atccacgaga cattcgcatg atcgatccgc
     3361 gtgaccctat tcgaggagat ccccgtggaa tatctggtcg tcttaatggg acctctgaaa
     3421 tgtggggtca tcatccacaa atgtcccata accagttgca aggtatcaac aaaatggttg
     3481 gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc tggctcgggc atcggtcctg
     3541 gaggtcccgg tcctagtaca gtatcaggca atatcccaac acagtggggg cctgctcaac
     3601 cggtaagcgt tggtgtaagt ggtcccaaag acatgtcaaa acagataagt ggatgggagg
     3661 aaccatcacc accgcctcag cgtcgcagta ttcctaacta cgatgatggt acatcgttgt
     3721 ggggtcagca aactcgtgtt cccgctgcaa gcggtcactg gaaagacatg actgattcga
     3781 taggtcgtag tagtcatctc atgcgtggcc aaagccaaac gggaggtata ggaatagccg
     3841 gcgttggaaa tagcaatgtt ccagtgggag ccaatccaag taatcctata agcagtgtag
     3901 ttggacctca agcccggatt ccatctgtgg gcggcgtaca acacaaacca gacggcggcg
     3961 ctatgtgggt gcattccggc aatgtaggtg gcagaaataa tgttgctgct gttactactt
     4021 ggggagatga cactcatagc gttaatgtcg gcgctcccag cagtggcagt gtatccagca
     4081 acaattgggt tgatgacaag tccaactcaa ccttggcaca aaactcttgg agcgacccgg
     4141 cccctgttgg agttagttgg ggcaataagc aaagcaaacc gccaagcaat agtgcttcat
     4201 caggttggag cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca
     4261 cgcacggggg gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg
     4321 gaatggtgaa cgtaattaac gcggagatca ttaagcaaag caagcaatac aggatccttg
     4381 tcgagaacgg ctttaaaaag gaagatgtag agcgggcatt agtgattgct aatatgaaca
     4441 tcgaagaggc agccgatatg ctccgtgcca actcatccct atcaatggat ggttggcgtc
     4501 gacatgatga gtcccttgga tcttatgccg accacaatag ttcaacaagc agcggtggat
     4561 ttgctggtcg ttacccggtc aacagtggac aaccttcaat gtcctttcct cataataacc
     4621 ttatgaataa catgggaggt accgctgtta ctggaggtaa caacaataca aacatgacag
     4681 ctttacaggt gcaaaagtat ttaaatcaag ggcaacatgg tgtcgctgtt ggaccgcaag
     4741 ccgttggtaa ttcttcagca gtatctgtcg gatttggtca gaacacgtct aacgcagcag
     4801 tggcaggagc agcctctgta aatatagcag caaatacaaa caaccaaccg tctggtcagc
     4861 aaattcgcat gctaggccag caaattcagt tggccattca tagtggtttc atatctagtc
     4921 agatattgac tcaaccgcta actcaaacaa cccttaacct tttaaaccaa cttcttagca
     4981 atattaagca tctccaggct gcgcagcaat cccttacccg cgggggaaat gtcaatccaa
     5041 tggcagtgaa tgtggctata tctaaataca agcagcaaat ccagaattta cagaaccaga
     5101 taaatgcaca acaggctgtg tatgtaaaac agcaaaatat gcaaccaact tcacaacaac
     5161 aacagcccca acaacagcaa cttccttctg ttcatctaag taactcaggc aacgactatt
     5221 taagaggtca cgatgcaata aataatttgc aaagcaactt ttctgagctc aatattaata
     5281 agccaagtgg atatcaagga gcgtccaatc aacaatcccg attaaatcag tggaagcttc
     5341 cagtattaga taaggagatc aactctgaca gtacggaatt ttctcgtgcc ccaggtgcaa
     5401 cgaaacaaaa tttgacggcc aacacaagca acataaactc tttgggtctt caaaacgata
     5461 gtacatggtc aactggacgc agtattggtg acggttggcc tgatccctca tctgataacg
     5521 agaataaaga ctggtctgtt gctcagccaa cttcagcagc aactgcttac actgatctgg
     5581 tccaagagtt tgagccaggc aagccatgga agggttcaca gatcaaaagc atagaagatg
     5641 atcccagcat tacaccagga agcgttgcta gatctccatt gtctattaat tcgacgccaa
     5701 aagatgctga catatttgcc aataccggta aaaattcacc gactgattta ccgccactaa
     5761 gtttatcgtc gtctacatgg agttttaatc caaaccaaaa ttatccgagt cacagttggt
     5821 ctgacaatag tcaacaatgt accgccactt cggagctttg gacaagcccg ctaaataaat
     5881 catcgtctcg aggtcccccg ccaggattga ctgccaattc aaataaatct gcaaatagta
     5941 atgcgtcaac gccaacaact attaccggag gtgcgaatgg atggttacag cctcgaagtg
     6001 gcggtgttca aaccacaaac actaattgga caggtggtaa caccacttgg ggcccacaaa
     6061 ctccttcctc tacaagctct agtggaacta gtggtggcaa cgtcggaggc gtcggcactt
     6121 cagccaataa tgcaaacagt ggttctgcag cttgtctgtc cggaaacaat agcggcaacg
     6181 gaaacggcag cgcgagcggc gccggcagcg gcaacaatgg caacagtagc tgcaacaaca
     6241 gtgccgccgg ggggggcagc agcagcaaca acacgattac cactgtagca aattcgaatc
     6301 ttgttggttc tagtggctct gtctcaaatt cctctggcgt tactgctaac tctagtactg
     6361 tttctgtagt tagttgtaca gcgagtggga attccataaa tggggcaggt actgcaaaca
     6421 gttctggttc aaagagtagt gcaaacaatt tagctagcgg ccagtctagc gcttctaact
     6481 taactaatag caccaattca acatggcgac aaactagcca aaaccaagct cttcaaagtc
     6541 aaagcaggcc atcaggcaga gaagctgact ttgattatat atctctcgtt tattccattg
     6601 ttgatgatta agcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     6661 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     6721 ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc
     6781 gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6841 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6901 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     6961 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     7021 ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     7081 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     7141 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     7201 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     7261 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     7321 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     7381 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     7441 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     7501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     7741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7981 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8581 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     8641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     8941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9301 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9361 gt
//
