LOCUS       sequence-288677-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSMG5-PINSMG6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Huntzinger E, Kashima I, Fauser M, Sauliere J, Izaurralde E
  TITLE     SMG6 is the catalytic endonuclease that cleaves mRNAs containing 
            nonsense codons in metazoan.
  JOURNAL   RNA. 2008 Dec . 14(12):2609-17.
  PUBMED    18974281
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Dec . 14(12):2609-17."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSMG5-PINSMG6-V5His6_E
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6335..6376
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6386..6403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6426..6443)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6476..6610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6500..6519)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6554..6573
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7232..7820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7312..7331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7991..8782,8783..8851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8614..8633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8852..8956)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9024..9042
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatggaggt gacattcagc tcgggcggat catcgaacgc
     2761 ctccagcgag tgcgccatag acggcggcac gaatcgctgt cgcggcttgg agcccaacaa
     2821 tggaacctgc atcctcagcc aggaggtaaa ggatctttac agatccctct acaccgcctc
     2881 caagcaactg gacgatgcca agaggaatgt gcagtcggtg ggacagctgt tccagcacga
     2941 aatcgaagag aaacgctcgc tcctagtgca gctgtgcaag cagatcatct tcaaggacta
     3001 ccagtcggtg ggcaagaagg tgcgcgaggt gatgtggcga cgtggatact acgagttcat
     3061 tgccttcgtg aagaagaact ggcacaagca gtgccaggat gcggccacgg cccaagagaa
     3121 catgcaaaag ctggagcgat tcctctgcgc tggcatcttc aactacaaac gattgtcggc
     3181 acgcatggaa gagatctatg atctggatct aaagtatttg attgatttct ccataatcag
     3241 tgatggctac ataagcgaca tgatcggaga caccatggag tcatcgcact cgggcaccca
     3301 cgcggtggac aagagtctgg aggccgtgtc ctttgcactc gacaccatac actcatcgct
     3361 gctcagtctg ggcgatcttc atcgctactt tctagacttt cgcatcgaca gcaaactaag
     3421 cataagcaag gagcaggtgg ccaagtatta tctggaggcc ttcaagctta atccggccat
     3481 tggcatggcc cagaaccagt tgggtacact gcactacgga cagaatcatg acctggactc
     3541 cacttaccac tacttgtact cccttgtgtg catcatacca ttcgaactaa gcgaaaataa
     3601 tcttaacaag ctgttcgcca agcacaccga atacctagag cgtatggatc cagaaaaact
     3661 agacttcaac atcgaggact tctttgcccg tttctatctg attgtggaca tatttttctt
     3721 cgacaaggaa gtgccggact ttaacgctct gtgccactgt gtgctgattg atctacgcaa
     3781 gatcctgtgc tcccagcagc tgcgcgttcc ggagccaagc atcttcaaga ttgtctccat
     3841 tctgttcttc tgtctgtcca agcttaagat gatcaactca ccaaaggttt actcgctgca
     3901 cgcctttctg gttgctgcct gttccgatct gatggacgcc tgcatcgtca gcatcgagca
     3961 gacaatcctg gctcgcgccc aagacaacga gcgcttccag gcggagtacg aggaacgctt
     4021 tcaggagttc gatactgtgg tgcgcaagtc ccggaatgag tacaagaact ggctagctgc
     4081 agtaggcgaa tgtgagcgca aggacaagaa gttgatgcca cgcccgcact ctctaaagga
     4141 ttgcagttcg gattcgcggg acagccagca ctctggcgag gaggcaaaga cccaggaggc
     4201 tgctgacggc gacaagatcg gtgaggccgt ttccacgcgc tccagtgacg aggccaagga
     4261 gtccgacctt aagaccccgc tctcctgcaa gagcaagcga cggggaatgc gtctacgccg
     4321 ccgacgccgc aagatcgccc agtcggacga cagctcctgc tatgacagcg aaagcgaact
     4381 ggacacagac ttttacagcg acgaggagga ttacacagat ctaagttcta actttgacac
     4441 cgatttcagc gacattgatg cagctgatcc tggggagcca tgcggcgaag agcgctatgc
     4501 agcagctaat tacagcaaaa aggagcgcaa agctggcggc ggcggcggag gaggagtcgg
     4561 tggcgtggtg tactccgaca acgaagacgt tgttattgag gaggagaaaa tcgtgtatcc
     4621 caacgaagta gagcgcagat ccaactcaca ggaggcggac tctgaggagt tgctgcgcag
     4681 ctttgaggta ctgcagctga actttaagag cgcagaggat gctcaaagca agccggtggc
     4741 acctccgccc gtcagctttt cagagccacc aaagaagctg gtttatcgca acaagtacac
     4801 caagatcaat cccaacatag tcattgactg tctgcacaat gaaccgacca ttagagccct
     4861 gaaaatgctt tttgactggc tgcgaatcaa cccagagatt ctcatcggct gctaccactc
     4921 gaatccggag tttgtgcaca agatcatgaa gcttctcaac tactgcaaca tcgacatctt
     4981 caccaggaag gtttacttcg agcgcgacct tctgaccact gccaacgtgc gtgattctct
     5041 ggtcgagtta ttcgatgtgc gcgccaacgt tccgctcacc gaggactttg ctttcaagaa
     5101 ctttgacatc ttccagagca cccagattac actggactgg gagaccatta tccgggagcg
     5161 tgtcactccc caggaagagt ccattctccg tattttcaaa atggtcgact ttggcttttt
     5221 catttgcaag cagaagaagt ttaactacag attctgcgtc aagacgcgtc gtttcaccga
     5281 gacccaaaag aagaaacagc gcgaggagaa gaagggaggc ggatcacgtc gccgcgagcg
     5341 tcgtaccgct ccgaagacca accgaacaaa gcggaacgaa ggacgcacac gtcgctactc
     5401 cgatcgcaag aacggcatcg tccgcaagag ctacaccagt aacgaggaaa aggacaccgt
     5461 ggaggagtgc cgcaagatgc cacgcaaggg ttacctaagg aatcgcaatg ccgagaaggc
     5521 ggctgctgtt ataaccagtg ccacgggaac ctctgtcgcc ggcggaacct ccgcctccac
     5581 aaccacaaac agtgtcatcg agaaactgaa tttgcagtcc agctcggggg ccgacgaaga
     5641 gcgctcggag gccaagtcga agaagtgtga gctcatgggc aagctgtggc tgcaaaacga
     5701 ggtggagact ttggaggagg agctgcggga cagtggcggc cgcatataca tcgaggtgcg
     5761 accgcgctat cttctaccgg ataccaactg ctttatcgac tgccttgaag actttgaaaa
     5821 actgtccacg gagtttaaac gctacacact tattatacca ctgacagtgg ttaaggaact
     5881 ggatggcctc tccaagggcg ttaagttgga ctcgtaccgc agttcgaagc agacgcagcg
     5941 catccaccac tttgacgagg tgtcgtcgcg ggccaaaaag tcgttagagt tcatcaagtc
     6001 ggccaaaggc aatgtgaaat gtgctaccac aaagggctcg tttgtcaatg catcggtgtt
     6061 tgccctagtt gaggaggaat atttgtccaa tgacgacaag attctggcaa cggcaatggc
     6121 tgtctcgaaa acggcaaaaa ccgaacaatg cacagcagat ggcaagtgtt tcattcaaac
     6181 ggaattggtg ctcattacaa ccgatcgtaa tctgcgtgtg aaggctcttt ctcgcaattt
     6241 ggccgtcagc gcgttggatg agtttctgca atgggccaag gattgccgcg aatcaaccgg
     6301 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     6361 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     6421 atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc
     6481 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6541 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6601 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6661 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc
     6721 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     6781 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     6841 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     6901 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     6961 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     7021 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     7081 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     7141 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag caaaaggcca
     7201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8281 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     8341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8581 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     8641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9001 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
