LOCUS       sequence-288678-        8999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSMG5-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Huntzinger E, Kashima I, Fauser M, Sauliere J, Izaurralde E
  TITLE     SMG6 is the catalytic endonuclease that cleaves mRNAs containing 
            nonsense codons in metazoan.
  JOURNAL   RNA. 2008 Dec . 14(12):2609-17.
  PUBMED    18974281
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Dec . 14(12):2609-17."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSMG5-siRNAres-V5His6_E
FEATURES             Location/Qualifiers
     source          1..8999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6284..6325
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6335..6352
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6375..6392)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6425..6559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6449..6468)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6503..6522
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7181..7769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7261..7280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7940..8731,8732..8800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8563..8582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8801..8905)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8973..8991
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatggaggt gacattcagc tcgggcggat catcgaacgc
     2761 ctccagcgag tgcgccatag acggcggcac gaatcgctgt cgcggcttgg agcccaacaa
     2821 tggaacctgc atcctcagcc aggaggtaaa ggatctttac agatccctct acaccgcctc
     2881 caagcaactg gacgatgcca agaggaatgt gcagtcggtg ggacagctgt tccaacatga
     2941 gattgaggaa aagcgctcgc tcctagtgca gctgtgcaag cagatcatct tcaaggacta
     3001 ccagtcggtg ggcaagaagg tgcgcgaggt gatgtggcga cgtggatact acgagttcat
     3061 tgccttcgtg aagaagaact ggcacaagca gtgccaggat gcggccacgg cccaagagaa
     3121 catgcaaaag ctggagcgat tcctctgcgc tggcatcttc aactacaaac gattgtcggc
     3181 acgcatggaa gagatctatg atctggatct aaagtatttg attgatttct ccataatcag
     3241 tgatggctac ataagcgaca tgatcggaga caccatggag tcatcgcact cgggcaccca
     3301 cgcggtggac aagagtctgg aggccgtgtc ctttgcactc gacaccatac actcatcgct
     3361 gctcagtctg ggcgatcttc atcgctactt tctagacttt cgcatcgaca gcaaactaag
     3421 cataagcaag gagcaggtgg ccaagtatta tctggaggcc ttcaagctta atccggccat
     3481 tggcatggcc cagaaccagt tgggtacact gcactacgga cagaatcatg acctggactc
     3541 cacttaccac tacttgtact cccttgtgtg catcatacca ttcgaactaa gcgaaaataa
     3601 tcttaacaag ctgttcgcca agcacaccga atacctagag cgtatggatc cagaaaaact
     3661 agacttcaac atcgaggact tctttgcccg tttctatctg attgtggaca tatttttctt
     3721 cgacaaggaa gtgccggact ttaacgctct gtgccactgt gtgctgattg atctacgcaa
     3781 gatcctgtgc tcccagcagc tgcgcgttcc ggagccaagc atcttcaaga ttgtctccat
     3841 tctgttcttc tgtctgtcca agcttaagat gatcaactca ccaaaggttt actcgctgca
     3901 cgcctttctg gttgctgcct gttccgatct gatggacgcc tgcatcgtca gcatcgagca
     3961 gacaatcctg gctcgcgccc aagacaacga gcgcttccag gcggagtacg aggaacgctt
     4021 tcaggagttc gatactgtgg tgcgcaagtc ccggaatgag tacaagaact ggctagctgc
     4081 agtaggcgaa tgtgagcgca aggacaagaa gttgatgcca cgcccgcact ctctaaagga
     4141 ttgcagttcg gattcgcggg acagccagca ctctggcgag gaggcaaaga cccaggaggc
     4201 tgctgacggc gacaagatcg gtgaggccgt ttccacgcgc tccagtgacg aggccaagga
     4261 gtccgacctt aagaccccgc tctcctgcaa gagcaagcga cggggaatgc gtctacgccg
     4321 ccgacgccgc aagatcgccc agtcggacga cagctcctgc tatgacagcg aaagcgaact
     4381 ggacacagac ttttacagcg acgaggagga ttacacagat ctaagttcta actttgacac
     4441 cgatttcagc gacattgatg cagctgatcc tggggagcca tgcggcgaag agcgctatgc
     4501 agcagctaat tacagcaaaa aggagcgcaa agctggcggc ggcggcggag gaggagtcgg
     4561 tggcgtggtg tactccgaca acgaagacgt tgttattgag gaggagaaaa tcgtgtatcc
     4621 caacgaagta gagcgcagat ccaactcaca ggaggcggac tctgaggagt tgctgcgcag
     4681 ctttgaggta ctgcagctga actttaagag cgcagaggat gctcaaagca agccggtggc
     4741 acctccgccc gtcagctttt cagagccacc aaagaagctg gtttatcgca acaagtacac
     4801 caagatcaat cccaacatag tcattgactg tctgcacaat gaaccgacca ttagagccct
     4861 gaaaatgctt tttgactggc tgcgaatcaa cccagagatt ctcatcggct gctaccactc
     4921 gaatccggag tttgtgcaca agatcatgaa gcttctcaac tactgcaaca tcgacatctt
     4981 caccaggaag gtttacttcg agcgcgacct tctgaccact gccaacgtgc gtgattctct
     5041 ggtcgagtta ttcgatgtgc gcgccaacgt tccgctcacc gaggactttg ctttcaagaa
     5101 ctttgacatc ttccagagca cccagattac actggactgg gagaccatta tccgggagcg
     5161 tgtcactccc caggaagagt ccattctccg tattttcaaa atggtcgact ttggcttttt
     5221 catttgcaag cagaagaagt ttaactacag attctgcgtc aagacgcgtc gtttcaccga
     5281 gacccaaaag aagaaacagc gcgaggagaa gaagggaggc ggatcacgtc gccgcgagcg
     5341 tcgtaccgct ccgaagacca accgaacaaa gcggaacgaa ggacgcacac gtcgctactc
     5401 cgatcgcaag aacggcatcg tccgcaagag ctacaccagt aacgaggaaa aggacaccgt
     5461 ggaggagtgc cgcaagatgc cacgcaaggg ttacctaagg aatcgcaatg ccgagaaggc
     5521 ggctgctgtt ataaccagtg ccacgggaac ctctgtcgcc ggcggaacct ccgcctccac
     5581 aaccacaaac agtgtcatcg agaaactgaa tttgcagtcc agctcggggg ccgacgaaga
     5641 gcgctcggag gccaagtcga agaagtgtga gctcatgggc aagctgtggc tgcaaaacga
     5701 ggtggagact ttggaggagg agctgcggga cagtcccgct catgtggtga tgtccccatt
     5761 tgtggtggtc gattcgaagg cattgaccga atacaatggc atagtcaagt cgctggtgag
     5821 gaccaagaag tgcattgtcc tcattccaaa tgcagtgctc aatgagttgg atgacttgaa
     5881 gaaacgcagc gagaatgtgc gccatgtcat ccgttggctg gagcaggagt ttaaacaggg
     5941 caataggcac ttgcgtgccc agcgcgataa cgaaagtcag ccgctatccc tgctgaagat
     6001 atcacgcaag atggatcgtg acgcctcagt tttccttcaa attgcccagt tctgcaatca
     6061 cttggtggcc aaccatgccg atccgcacgt cagcgaactg cagaagagcg tcgtcacgta
     6121 cttgtccggc gataatttgc acgagaagaa ccgccagcaa cagaacttca gctacaccgg
     6181 catccttgac tcgatacccg tacgctatgc ccaaatccag agcttctacg caaaatttaa
     6241 ggccaacaaa aaacgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     6301 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     6361 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
     6421 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6481 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6541 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6601 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6661 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6721 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6781 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     6841 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     6901 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     6961 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     7021 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     7081 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagccagc
     7141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     7381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8221 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     8281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8521 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     8581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8941 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
