LOCUS       sequence-288680-        7766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSMG6-delPIN-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Huntzinger E, Kashima I, Fauser M, Sauliere J, Izaurralde E
  TITLE     SMG6 is the catalytic endonuclease that cleaves mRNAs containing 
            nonsense codons in metazoan.
  JOURNAL   RNA. 2008 Dec . 14(12):2609-17.
  PUBMED    18974281
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Dec . 14(12):2609-17."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSMG6-delPIN-siRNAres-V5Hi...
FEATURES             Location/Qualifiers
     source          1..7766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5051..5092
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5102..5119
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5142..5159)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5192..5326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5216..5235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5270..5289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5948..6536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6028..6047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6707..7498,7499..7567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7330..7349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7568..7672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7740..7758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgagtcg aaagggaaca cgagaacaaa atgatatatc
     2761 gctgataatt gggacgctgc cgaaacgcct gcagaacaaa atcgcggcca gctgcagcca
     2821 cgcagcagca gcggcaacaa caacaaaggc gttggagacg gaaaacgctg agaatgcgga
     2881 gcccgtggct acgacagcaa atgcttcaag gccagtcgaa atcctgagca atgctagcac
     2941 aggaggaggc aaacccgatg cccagtcaaa gcaacccgtt ttggatacca gcgggaaaat
     3001 ggcgtgggag ggcgggattc caggcatcct gcgcgtgacc acctcggtgg cgggcgatgt
     3061 taacaggaac ggacagggtc agggcaagaa caagagcggt aacagtgctc ggcgcagggg
     3121 tcgcaaggac aaggagcacg ctacgcagtc gccagtgacg cgcacgcgct tgaccaccga
     3181 gcatccggat tggtttgagc cgctgttggg tctgccccat gccaagaata tagtccatct
     3241 gtacgatcag ctcatggtcc tcaatcagag gaatctcata ctgataaact ggaagaactt
     3301 ctgctacatt cacgaccagc tgcaggacgc tttcaagcaa cttcttttgg agcaacttaa
     3361 gttcgtgtgc gagcacaagg ttgatgtctt cttttggaaa ctactcttct acaatgtgcg
     3421 gaattacttg aagcgccagc aaacggacca ggcgcacaca catacgctcc ttctcatcga
     3481 gcaagctatc aagttttacc gcatgctcta cgacaagctg atggccaagt gcgtggcatc
     3541 gtcgcgctgt gacagcgcac ttaaagtggt ggcccagagg ctgctcatct gtttgggcga
     3601 tctgacgcgc taccgggtta accatgtcaa ggccacagat tacatggagg cggccaggta
     3661 ctatcagagg gcgcaggaac tggtacctgg caacggggca cctttcaacc agctggcggt
     3721 catttccatt tatcaccaca agcgcttcga tgcagtttat tactatgtcc gcagcttgtt
     3781 gacttcgaac tctatccaat ctgccaagga aagcctgctg gatttgttcg acgagattcg
     3841 gcgtaagtac gaggagaccg agatgaagca atctcccatg cactatgcag ctggatcgaa
     3901 gaatcaaaag tcgaagcaga tgcgcaagga ggtatggatc tatccggatg ggatcagacg
     3961 cctacatcgc accgatgaca agggcaacaa ggcgaagggc aacaaggcca ctatggcaga
     4021 ggtcaatcgt tacgaggaga tgccgccgga ggagattctt cctcgcattg tttcccttta
     4081 tctgtactta ttaggcaaac tctacaccgg cacagatgtg gactcgttat acccgttgct
     4141 aaggaaactg caaatccaaa taggcgtcgc tttaaggcac gaaaacctgt tgtcacgctc
     4201 caaactgctg aaaatagtag cactcaactt gttcgtcgtc gagcacaaca aacccaaaac
     4261 gtcacgccgc gagatgcgtt atcacagctt caattttgcc aactcattct ttggactcat
     4321 gatgaagaaa acgaaccagc tgctggctgg ttttgtagag gattctacaa atgtccagtg
     4381 tctcccagaa gaagactttg cgactgtgaa cacataccta cagttcgtga atgtttatgt
     4441 acgttggttg agcatcagtt tggacgtgtg ggaacccgtc agatcggaag aacactcttt
     4501 catcgattgc tgggctgagc taacaatcct ttttgagtac attgaatgct taatggggaa
     4561 gcacaaactt gagaaaaacg aaatactgtt ggatgaggat gtggccctta gaggtttcac
     4621 tccgttgggt cgtgaaacgc tgagcatgga ctatctcaaa agaggatccg aacgtcttca
     4681 gtttttcgaa cgtgttcgta ggattggtca gttccaggaa tactacgtcc agcatcaaga
     4741 ggctctccag cagccgttgg aatcaagctt cactgtcgag cacctaaacg cagcgatgaa
     4801 gaacgcattg gaaaactatg atgctgaaag ttgtggtctt gcggcgaacg tttcaaagat
     4861 tcaatgtaaa gccgttgagg aattgttgtg tcgttcaact gattccgatg tttccgagct
     4921 gtgcaagctg aagaaagagc ttgaggccaa ggctagggtt aagcagatat gcaccagcaa
     4981 gttggaggac attctcaagt tcgtggacac gaaaggcggc cgctcgagtc tagagggccc
     5041 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     5101 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     5161 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     5221 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5281 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5341 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5401 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     5461 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     5521 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     5581 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     5641 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     5701 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     5761 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     5821 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     5881 gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6121 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     6181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6361 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7021 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7321 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7681 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     7741 ataggcgtat cacgaggccc tttcgt
//
