LOCUS       sequence-288685-        8294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSMG6-PINSMG5-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Huntzinger E, Kashima I, Fauser M, Sauliere J, Izaurralde E
  TITLE     SMG6 is the catalytic endonuclease that cleaves mRNAs containing 
            nonsense codons in metazoan.
  JOURNAL   RNA. 2008 Dec . 14(12):2609-17.
  PUBMED    18974281
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Dec . 14(12):2609-17."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSMG6-PINSMG5-siRNAres-V5H...
FEATURES             Location/Qualifiers
     source          1..8294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5579..5620
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5630..5647
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5670..5687)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5720..5854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5744..5763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5798..5817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6476..7064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6556..6575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7235..8026,8027..8095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7858..7877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8096..8200)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8268..8286
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgagtcg aaagggaaca cgagaacaaa atgatatatc
     2761 gctgataatt gggacgctgc cgaaacgcct gcagaacaaa atcgcggcca gctgcagcca
     2821 cgcagcagca gcggcaacaa caacaaaggc gttggagacg gaaaacgctg agaatgcgga
     2881 gcccgtggct acgacagcaa atgcttcaag gccagtcgaa atcctgagca atgctagcac
     2941 aggaggaggc aaacccgatg cccagtcaaa gcaacccgtt ttggatacca gcgggaaaat
     3001 ggcgtgggag ggcgggattc caggcatcct gcgcgtgacc acctcggtgg cgggcgatgt
     3061 taacaggaac ggacagggtc agggcaagaa caagagcggt aacagtgctc ggcgcagggg
     3121 tcgcaaggac aaggagcacg ctacgcagtc gccagtgacg cgcacgcgct tgaccaccga
     3181 gcatccggat tggtttgagc cgctgttggg tctgccccat gccaagaata tagtccatct
     3241 gtacgatcag ctcatggtcc tcaatcagag gaatctcata ctgataaact ggaagaactt
     3301 ctgctacatt cacgaccagc tgcaggacgc tttcaagcaa cttcttttgg agcaacttaa
     3361 gttcgtgtgc gagcacaagg ttgatgtctt cttttggaaa ctactcttct acaatgtgcg
     3421 gaattacttg aagcgccagc aaacggacca ggcgcacaca catacgctcc ttctcatcga
     3481 gcaagctatc aagttttacc gcatgctcta cgacaagctg atggccaagt gcgtggcatc
     3541 gtcgcgctgt gacagcgcac ttaaagtggt ggcccagagg ctgctcatct gtttgggcga
     3601 tctgacgcgc taccgggtta accatgtcaa ggccacagat tacatggagg cggccaggta
     3661 ctatcagagg gcgcaggaac tggtacctgg caacggggca cctttcaacc agctggcggt
     3721 catttccatt tatcaccaca agcgcttcga tgcagtttat tactatgtcc gcagcttgtt
     3781 gacttcgaac tctatccaat ctgccaagga aagcctgctg gatttgttcg acgagattcg
     3841 gcgtaagtac gaggagaccg agatgaagca atctcccatg cactatgcag ctggatcgaa
     3901 gaatcaaaag tcgaagcaga tgcgcaagga ggtatggatc tatccggatg ggatcagacg
     3961 cctacatcgc accgatgaca agggcaacaa ggcgaagggc aacaaggcca ctatggcaga
     4021 ggtcaatcgt tacgaggaga tgccgccgga ggagattctt cctcgcattg tttcccttta
     4081 tctgtactta ttaggcaaac tctacaccgg cacagatgtg gactcgttat acccgttgct
     4141 aaggaaactg caaatccaaa taggcgtcgc tttaaggcac gaaaacctgt tgtcacgctc
     4201 caaactgctg aaaatagtag cactcaactt gttcgtcgtc gagcacaaca aacccaaaac
     4261 gtcacgccgc gagatgcgtt atcacagctt caattttgcc aactcattct ttggactcat
     4321 gatgaagaaa acgaaccagc tgctggctgg ttttgtagag gattctacaa atgtccagtg
     4381 tctcccagaa gaagactttg cgactgtgaa cacataccta cagttcgtga atgtttatgt
     4441 acgttggttg agcatcagtt tggacgtgtg ggaacccgtc agatcggaag aacactcttt
     4501 catcgattgc tgggctgagc taacaatcct ttttgagtac attgaatgct taatggggaa
     4561 gcacaaactt gagaaaaacg aaatactgtt ggatgaggat gtggccctta gaggtttcac
     4621 tccgttgggt cgtgaaacgc tgagcatgga ctatctcaaa agaggatccg aacgtcttca
     4681 gtttttcgaa cgtgttcgta ggattggtca gttccaggaa tactacgtcc agcatcaaga
     4741 ggctctccag cagccgttgg aatcaagctt cactgtcgag cacctaaacg cagcgatgaa
     4801 gaacgcattg gaaaactatg atgctgaaag ttgtggtctt gcggcgaacg tttcaaagat
     4861 tcaatgtaaa gccgttgagg aattgttgtg tcgttcaact gattccgatg tttccgagct
     4921 gtgcaagctg aagaaagagc ttgaggccaa ggctagggtt aagcagatat gcaccagcaa
     4981 gttggaggac attctcaagt tcgtggacac gaaaggcggc cgccccgctc atgtggtgat
     5041 gtccccattt gtggtggtcg attcgaaggc attgaccgaa tacaatggca tagtcaagtc
     5101 gctggtgagg accaagaagt gcattgtcct cattccaaat gcagtgctca atgagttgga
     5161 tgacttgaag aaacgcagcg agaatgtgcg ccatgtcatc cgttggctgg agcaggagtt
     5221 taaacagggc aataggcact tgcgtgccca gcgcgataac gaaagtcagc cgctatccct
     5281 gctgaagata tcacgcaaga tggatcgtga cgcctcagtt ttccttcaaa ttgcccagtt
     5341 ctgcaatcac ttggtggcca accatgccga tccgcacgtc agcgaactgc agaagagcgt
     5401 cgtcacgtac ttgtccggcg ataatttgca cgagaagaac cgccagcaac agaacttcag
     5461 ctacaccggc atccttgact cgatacccgt acgctatgcc caaatccaga gcttctacgc
     5521 aaaatttaag gccaacaaaa aaggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     5581 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     5641 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg
     5701 ctgatcagcc tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac
     5761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     5821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     5881 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     5941 tggtatggct gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct
     6001 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     6061 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     6121 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     6181 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     6241 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     6301 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     6361 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     6421 ctcttaccag ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6661 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6901 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7141 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7201 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7261 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7321 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7381 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7441 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7501 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     7561 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7621 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7681 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7741 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7801 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     7861 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7921 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7981 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8041 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8101 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8281 cgaggccctt tcgt
//
