LOCUS       sequence-288698-        9534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delmf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delmf1_E
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6819..6860
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6870..6887
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6910..6927)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6960..7094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6984..7003)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7038..7057
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7716..8304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7796..7815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8475..9266,9267..9335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9098..9117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9336..9440)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9508..9526
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgcg gtccaccaat
     2761 gtggaagcca agcattaaca atggtactga tctttgggag tccaatttga gaaacggagg
     2821 tcagccggcc gcacagcaag ttccaaagcc gtcgtggggt catacaccat cctctaactt
     2881 aggtggaaca tggggtgagg acgacgatgg cgccgatagt agtagtgtgt ggactggagg
     2941 agctgttagc aacgcgggat ccggagctgc agtgggagta aaccaagccg gagttaatgt
     3001 cggtccaggc ggtgttgttt cgtctggcgg acctcagtgg ggacaaggtg tcgttggcgt
     3061 cggacttgga tcaactggag gtaacgggtc aagcaatata actggatcgt ctggagtcgc
     3121 aacaggtagt agcggaaact ccagcaacgc tggtaacggt tggggagacc ctcgtgaaat
     3181 acgccctttg ggagttggtg gctccatgga tattcgaaat gttgaacatc gcggcggtaa
     3241 cggttctgga gcaacttcga gcgatccacg agacattcgc atgatcgatc cgcgtgaccc
     3301 tattcgagga gatccccgtg gaatatctgg tcgtcttaat gggacctctg aaatgtgggg
     3361 tcatcatcca caaatgtccc ataaccagtt gcaaggtatc aacaaaatgg ttggtcaaag
     3421 tgtagcaact gccagcacca gtgtcggaac atctggctcg ggcatcggtc ctggaggtcc
     3481 cggtcctagt acagtatcag gcaatatccc aacacagtgg gggcctgctc aaccggtaag
     3541 cgttggtgta agtggtccca aagacatgtc aaaacagata agtggatggg aggaaccatc
     3601 accaccgcct cagcgtcgca gtattcctaa ctacgatgat ggtacatcgt tgtggggtca
     3661 gcaaactcgt gttcccgctg caagcggtca ctggaaagac atgactgatt cgataggtcg
     3721 tagtagtcat ctcatgcgtg gccaaagcca aacgggaggt ataggaatag ccggcgttgg
     3781 aaatagcaat gttccagtgg gagccaatcc aagtaatcct ataagcagtg tagttggacc
     3841 tcaagcccgg attccatctg tgggcggcgt acaacacaaa ccagacggcg gcgctatgtg
     3901 ggtgcattcc ggcaatgtag gtggcagaaa taatgttgct gctgttacta cttggggaga
     3961 tgacactcat agcgttaatg tcggcgctcc cagcagtggc agtgtatcca gcaacaattg
     4021 ggttgatgac aagtccaact caaccttggc acaaaactct tggagcgacc cggcccctgt
     4081 tggagttagt tggggcaata agcaaagcaa accgccaagc aatagtgctt catcaggttg
     4141 gagcactgct gcgggcgtgg tggatggggt tgatctagga tctgagtgga acacgcacgg
     4201 ggggattatt ggaaaatctc agcaacaaca aaaactagcg ggacttaacg tgggaatggt
     4261 gaacgtaatt aacgcggaga tcattaagca aagcaagcaa tacaggatcc ttgtcgagaa
     4321 cggctttaaa aaggaagatg tagagcgggc attagtgatt gctaatatga acatcgaaga
     4381 ggcagccgat atgctccgtg ccaactcatc cctatcaatg gatggttggc gtcgacatga
     4441 tgagtccctt ggatcttatg ccgaccacaa tagttcaaca agcagcggtg gatttgctgg
     4501 tcgttacccg gtcaacagtg gacaaccttc aatgtccttt cctcataata accttatgaa
     4561 taacatggga ggtaccgctg ttactggagg taacaacaat acaaacatga cagctttaca
     4621 ggtgcaaaag tatttaaatc aagggcaaca tggtgtcgct gttggaccgc aagccgttgg
     4681 taattcttca gcagtatctg tcggatttgg tcagaacacg tctaacgcag cagtggcagg
     4741 agcagcctct gtaaatatag cagcaaatac aaacaaccaa ccgtctggtc agcaaattcg
     4801 catgctaggc cagcaaattc agttggccat tcatagtggt ttcatatcta gtcagatatt
     4861 gactcaaccg ctaactcaaa caacccttaa ccttttaaac caacttctta gcaatattaa
     4921 gcatctccag gctgcgcagc aatcccttac ccgcggggga aatgtcaatc caatggcagt
     4981 gaatgtggct atatctaaat acaagcagca aatccagaat ttacagaacc agataaatgc
     5041 acaacaggct gtgtatgtaa aacagcaaaa tatgcaacca acttcacaac aacaacagcc
     5101 ccaacaacag caacttcctt ctgttcatct aagtaactca ggcaacgact atttaagagg
     5161 tcacgatgca ataaataatt tgcaaagcaa cttttctgag ctcaatatta ataagccaag
     5221 tggatatcaa ggagcgtcca atcaacaatc ccgattaaat cagtggaagc ttccagtatt
     5281 agataaggag atcaactctg acagtacgga attttctcgt gccccaggtg caacgaaaca
     5341 aaatttgacg gccaacacaa gcaacataaa ctctttgggt cttcaaaacg atagtacatg
     5401 gtcaactgga cgcagtattg gtgacggttg gcctgatccc tcatctgata acgagaataa
     5461 agactggtct gttgctcagc caacttcagc agcaactgct tacactgatc tggtccaaga
     5521 gtttgagcca ggcaagccat ggaagggttc acagatcaaa agcatagaag atgatcccag
     5581 cattacacca ggaagcgttg ctagatctcc attgtctatt aattcgacgc caaaagatgc
     5641 tgacatattt gccaataccg gtaaaaattc accgactgat ttaccgccac taagtttatc
     5701 gtcgtctaca tggagtttta atccaaacca aaattatccg agtcacagtt ggtctgacaa
     5761 tagtcaacaa tgtaccgcca cttcggagct ttggacaagc ccgctaaata aatcatcgtc
     5821 tcgaggtccc ccgccaggat tgactgccaa ttcaaataaa tctgcaaata gtaatgcgtc
     5881 aacgccaaca actattaccg gaggtgcgaa tggatggtta cagcctcgaa gtggcggtgt
     5941 tcaaaccaca aacactaatt ggacaggtgg taacaccact tggggctcca gttggttgct
     6001 tttgaaaaat ctaacagcac agattgatgg tcctactttg cgtacactgt gtatgcagca
     6061 tgggcccctt gtcagctttc acccgtattt gaaccaagga attgccttat gtaaatatac
     6121 tactcgtgag gaggcgaaca aggcgcaaat ggcgttaaac aactgtgtcc tcgccaacac
     6181 cacaatattt gctgaatctc ccagcgagaa cgaggtgcaa agcattatgc agcacttacc
     6241 acaaactcct tcctctacaa gctctagtgg aactagtggt ggcaacgtcg gaggcgtcgg
     6301 cacttcagcc aataatgcaa acagtggttc tgcagcttgt ctgtccggaa acaatagcgg
     6361 caacggaaac ggcagcgcga gcggcgccgg cagcggcaac aatggcaaca gtagctgcaa
     6421 caacagtgcc gccggggggg gcagcagcag caacaacacg attaccactg tagcaaattc
     6481 gaatcttgtt ggttctagtg gctctgtctc aaattcctct ggcgttactg ctaactctag
     6541 tactgtttct gtagttagtt gtacagcgag tgggaattcc ataaatgggg caggtactgc
     6601 aaacagttct ggttcaaaga gtagtgcaaa caatttagct agcggccagt ctagcgcttc
     6661 taacttaact aatagcacca attcaacatg gcgacaaact agccaaaacc aagctcttca
     6721 aagtcaaagc aggccatcag gcagagaagc tgactttgat tatatatctc tcgtttattc
     6781 cattgttgat gattaatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     6841 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     6901 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     6961 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7021 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7081 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     7141 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     7201 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     7261 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     7321 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     7381 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     7441 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     7501 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     7561 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     7621 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag
     7681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     7921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8341 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8401 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8761 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     8821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9061 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     9121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     9481 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
