LOCUS       sequence-288700-        8933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delQ_E
FEATURES             Location/Qualifiers
     source          1..8933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6218..6259
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6269..6286
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6309..6326)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6359..6493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6383..6402)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6437..6456
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7115..7703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7195..7214)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7874..8665,8666..8734))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8497..8516
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8735..8839)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8907..8925
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggaggta acgggtcaag caatataact ggatcgtctg
     3181 gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg taacggttgg ggagaccctc
     3241 gtgaaatacg ccctttggga gttggtggct ccatggatat tcgaaatgtt gaacatcgcg
     3301 gcggtaacgg ttctggagca acttcgagcg atccacgaga cattcgcatg atcgatccgc
     3361 gtgaccctat tcgaggagat ccccgtggaa tatctggtcg tcttaatggg acctctgaaa
     3421 tgtggggtca tcatccacaa atgtcccata accagttgca aggtatcaac aaaatggttg
     3481 gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc tggctcgggc atcggtcctg
     3541 gaggtcccgg tcctagtaca gtatcaggca atatcccaac acagtggggg cctgctcaac
     3601 cggtaagcgt tggtgtaagt ggtcccaaag acatgtcaaa acagataagt ggatgggagg
     3661 aaccatcacc accgcctcag cgtcgcagta ttcctaacta cgatgatggt acatcgttgt
     3721 ggggtcagca aactcgtgtt cccgctgcaa gcggtcactg gaaagacatg actgattcga
     3781 taggtcgtag tagtcatctc atgcgtggcc aaagccaaac gggaggtata ggaatagccg
     3841 gcgttggaaa tagcaatgtt ccagtgggag ccaatccaag taatcctata agcagtgtag
     3901 ttggacctca agcccggatt ccatctgtgg gcggcgtaca acacaaacca gacggcggcg
     3961 ctatgtgggt gcattccggc aatgtaggtg gcagaaataa tgttgctgct gttactactt
     4021 ggggagatga cactcatagc gttaatgtcg gcgctcccag cagtggcagt gtatccagca
     4081 acaattgggt tgatgacaag tccaactcaa ccttggcaca aaactcttgg agcgacccgg
     4141 cccctgttgg agttagttgg ggcaataagc aaagcaaacc gccaagcaat agtgcttcat
     4201 caggttggag cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca
     4261 cgcacggggg gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg
     4321 gaatggtgaa cgtaattaac gcggagatca ttaagcaaag caagcaatac aggatccttg
     4381 tcgagaacgg ctttaaaaag gaagatgtag agcgggcatt agtgattgct aatatgaaca
     4441 tcgaagaggc agccgatatg ctccgtgcca actcatccct atcaatggat ggttggcgtc
     4501 gacatgatga gtcccttgga tcttatgccg accacaatag ttcaacaagc agcggtggat
     4561 ttgctggtcg ttacccggtc aacagtggac aaccttcaat gtcctttcct tatcaaggag
     4621 cgtccaatca acaatcccga ttaaatcagt ggaagcttcc agtattagat aaggagatca
     4681 actctgacag tacggaattt tctcgtgccc caggtgcaac gaaacaaaat ttgacggcca
     4741 acacaagcaa cataaactct ttgggtcttc aaaacgatag tacatggtca actggacgca
     4801 gtattggtga cggttggcct gatccctcat ctgataacga gaataaagac tggtctgttg
     4861 ctcagccaac ttcagcagca actgcttaca ctgatctggt ccaagagttt gagccaggca
     4921 agccatggaa gggttcacag atcaaaagca tagaagatga tcccagcatt acaccaggaa
     4981 gcgttgctag atctccattg tctattaatt cgacgccaaa agatgctgac atatttgcca
     5041 ataccggtaa aaattcaccg actgatttac cgccactaag tttatcgtcg tctacatgga
     5101 gttttaatcc aaaccaaaat tatccgagtc acagttggtc tgacaatagt caacaatgta
     5161 ccgccacttc ggagctttgg acaagcccgc taaataaatc atcgtctcga ggtcccccgc
     5221 caggattgac tgccaattca aataaatctg caaatagtaa tgcgtcaacg ccaacaacta
     5281 ttaccggagg tgcgaatgga tggttacagc ctcgaagtgg cggtgttcaa accacaaaca
     5341 ctaattggac aggtggtaac accacttggg gctccagttg gttgcttttg aaaaatctaa
     5401 cagcacagat tgatggtcct actttgcgta cactgtgtat gcagcatggg ccccttgtca
     5461 gctttcaccc gtatttgaac caaggaattg ccttatgtaa atatactact cgtgaggagg
     5521 cgaacaaggc gcaaatggcg ttaaacaact gtgtcctcgc caacaccaca atatttgctg
     5581 aatctcccag cgagaacgag gtgcaaagca ttatgcagca cttaccacaa actccttcct
     5641 ctacaagctc tagtggaact agtggtggca acgtcggagg cgtcggcact tcagccaata
     5701 atgcaaacag tggttctgca gcttgtctgt ccggaaacaa tagcggcaac ggaaacggca
     5761 gcgcgagcgg cgccggcagc ggcaacaatg gcaacagtag ctgcaacaac agtgccgccg
     5821 gggggggcag cagcagcaac aacacgatta ccactgtagc aaattcgaat cttgttggtt
     5881 ctagtggctc tgtctcaaat tcctctggcg ttactgctaa ctctagtact gtttctgtag
     5941 ttagttgtac agcgagtggg aattccataa atggggcagg tactgcaaac agttctggtt
     6001 caaagagtag tgcaaacaat ttagctagcg gccagtctag cgcttctaac ttaactaata
     6061 gcaccaattc aacatggcga caaactagcc aaaaccaagc tcttcaaagt caaagcaggc
     6121 catcaggcag agaagctgac tttgattata tatctctcgt ttattccatt gttgatgatt
     6181 aagcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     6241 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
     6301 ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga
     6361 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6421 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6481 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6541 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     6601 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     6661 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     6721 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     6781 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     6841 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     6901 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     6961 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     7021 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg
     7081 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7141 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7201 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7261 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     7321 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7381 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7441 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7501 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     7561 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7621 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7681 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7741 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7801 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7861 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7921 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7981 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8041 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8101 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8161 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     8221 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8281 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8341 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8401 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8461 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     8521 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8581 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8641 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8701 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8761 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8821 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8881 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgt
//
