LOCUS       sequence-288701-        9416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delUBA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delUBA_E
FEATURES             Location/Qualifiers
     source          1..9416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6701..6742
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6752..6769
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6792..6809)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6842..6976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6866..6885)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6920..6939
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7598..8186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7678..7697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8357..9148,9149..9217))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8980..8999
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9218..9322)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9390..9408
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggaggta acgggtcaag caatataact ggatcgtctg
     3181 gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg taacggttgg ggagaccctc
     3241 gtgaaatacg ccctttggga gttggtggct ccatggatat tcgaaatgtt gaacatcgcg
     3301 gcggtaacgg ttctggagca acttcgagcg atccacgaga cattcgcatg atcgatccgc
     3361 gtgaccctat tcgaggagat ccccgtggaa tatctggtcg tcttaatggg acctctgaaa
     3421 tgtggggtca tcatccacaa atgtcccata accagttgca aggtatcaac aaaatggttg
     3481 gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc tggctcgggc atcggtcctg
     3541 gaggtcccgg tcctagtaca gtatcaggca atatcccaac acagtggggg cctgctcaac
     3601 cggtaagcgt tggtgtaagt ggtcccaaag acatgtcaaa acagataagt ggatgggagg
     3661 aaccatcacc accgcctcag cgtcgcagta ttcctaacta cgatgatggt acatcgttgt
     3721 ggggtcagca aactcgtgtt cccgctgcaa gcggtcactg gaaagacatg actgattcga
     3781 taggtcgtag tagtcatctc atgcgtggcc aaagccaaac gggaggtata ggaatagccg
     3841 gcgttggaaa tagcaatgtt ccagtgggag ccaatccaag taatcctata agcagtgtag
     3901 ttggacctca agcccggatt ccatctgtgg gcggcgtaca acacaaacca gacggcggcg
     3961 ctatgtgggt gcattccggc aatgtaggtg gcagaaataa tgttgctgct gttactactt
     4021 ggggagatga cactcatagc gttaatgtcg gcgctcccag cagtggcagt gtatccagca
     4081 acaattgggt tgatgacaag tccaactcaa ccttggcaca aaactcttgg agcgacccgg
     4141 cccctgttgg agttagttgg ggcaataagc aaagcaaacc gccaagcaat agtgcttcat
     4201 caggttggag cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca
     4261 cgcacggggg gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg
     4321 gatcttatgc cgaccacaat agttcaacaa gcagcggtgg atttgctggt cgttacccgg
     4381 tcaacagtgg acaaccttca atgtcctttc ctcataataa ccttatgaat aacatgggag
     4441 gtaccgctgt tactggaggt aacaacaata caaacatgac agctttacag gtgcaaaagt
     4501 atttaaatca agggcaacat ggtgtcgctg ttggaccgca agccgttggt aattcttcag
     4561 cagtatctgt cggatttggt cagaacacgt ctaacgcagc agtggcagga gcagcctctg
     4621 taaatatagc agcaaataca aacaaccaac cgtctggtca gcaaattcgc atgctaggcc
     4681 agcaaattca gttggccatt catagtggtt tcatatctag tcagatattg actcaaccgc
     4741 taactcaaac aacccttaac cttttaaacc aacttcttag caatattaag catctccagg
     4801 ctgcgcagca atcccttacc cgcgggggaa atgtcaatcc aatggcagtg aatgtggcta
     4861 tatctaaata caagcagcaa atccagaatt tacagaacca gataaatgca caacaggctg
     4921 tgtatgtaaa acagcaaaat atgcaaccaa cttcacaaca acaacagccc caacaacagc
     4981 aacttccttc tgttcatcta agtaactcag gcaacgacta tttaagaggt cacgatgcaa
     5041 taaataattt gcaaagcaac ttttctgagc tcaatattaa taagccaagt ggatatcaag
     5101 gagcgtccaa tcaacaatcc cgattaaatc agtggaagct tccagtatta gataaggaga
     5161 tcaactctga cagtacggaa ttttctcgtg ccccaggtgc aacgaaacaa aatttgacgg
     5221 ccaacacaag caacataaac tctttgggtc ttcaaaacga tagtacatgg tcaactggac
     5281 gcagtattgg tgacggttgg cctgatccct catctgataa cgagaataaa gactggtctg
     5341 ttgctcagcc aacttcagca gcaactgctt acactgatct ggtccaagag tttgagccag
     5401 gcaagccatg gaagggttca cagatcaaaa gcatagaaga tgatcccagc attacaccag
     5461 gaagcgttgc tagatctcca ttgtctatta attcgacgcc aaaagatgct gacatatttg
     5521 ccaataccgg taaaaattca ccgactgatt taccgccact aagtttatcg tcgtctacat
     5581 ggagttttaa tccaaaccaa aattatccga gtcacagttg gtctgacaat agtcaacaat
     5641 gtaccgccac ttcggagctt tggacaagcc cgctaaataa atcatcgtct cgaggtcccc
     5701 cgccaggatt gactgccaat tcaaataaat ctgcaaatag taatgcgtca acgccaacaa
     5761 ctattaccgg aggtgcgaat ggatggttac agcctcgaag tggcggtgtt caaaccacaa
     5821 acactaattg gacaggtggt aacaccactt ggggctccag ttggttgctt ttgaaaaatc
     5881 taacagcaca gattgatggt cctactttgc gtacactgtg tatgcagcat gggccccttg
     5941 tcagctttca cccgtatttg aaccaaggaa ttgccttatg taaatatact actcgtgagg
     6001 aggcgaacaa ggcgcaaatg gcgttaaaca actgtgtcct cgccaacacc acaatatttg
     6061 ctgaatctcc cagcgagaac gaggtgcaaa gcattatgca gcacttacca caaactcctt
     6121 cctctacaag ctctagtgga actagtggtg gcaacgtcgg aggcgtcggc acttcagcca
     6181 ataatgcaaa cagtggttct gcagcttgtc tgtccggaaa caatagcggc aacggaaacg
     6241 gcagcgcgag cggcgccggc agcggcaaca atggcaacag tagctgcaac aacagtgccg
     6301 ccgggggggg cagcagcagc aacaacacga ttaccactgt agcaaattcg aatcttgttg
     6361 gttctagtgg ctctgtctca aattcctctg gcgttactgc taactctagt actgtttctg
     6421 tagttagttg tacagcgagt gggaattcca taaatggggc aggtactgca aacagttctg
     6481 gttcaaagag tagtgcaaac aatttagcta gcggccagtc tagcgcttct aacttaacta
     6541 atagcaccaa ttcaacatgg cgacaaacta gccaaaacca agctcttcaa agtcaaagca
     6601 ggccatcagg cagagaagct gactttgatt atatatctct cgtttattcc attgttgatg
     6661 attaagcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     6721 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
     6781 ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga
     6841 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     6901 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     6961 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     7021 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     7081 tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     7141 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     7201 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     7261 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     7321 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     7381 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     7441 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     7501 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agccagcaaa
     7561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     7801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8221 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8281 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8341 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8401 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8461 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8521 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8581 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8641 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     8701 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8761 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8821 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8881 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8941 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     9001 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9061 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9121 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9181 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9361 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
