LOCUS       sequence-288703-        7827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6A_Cterm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsTNRC6A_C-term_F
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4174..4185
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             5112..5153
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5163..5180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5203..5220)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5253..5387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5277..5296)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5331..5350
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6009..6597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6089..6108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6768..7559,7560..7628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7391..7410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7629..7733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7801..7819
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat cctgagtggc aaaaaaagga gaagggaaag
     2761 gggaatgatg aaaggtggaa acaaacaaga agaagcgtgg ataaatccat ttgttaaaca
     2821 gttttcaaac atcagttttt cgagagactc accagaggaa aatgtacaaa gcaataagat
     2881 ggacctttct ggaggaatgt tacaagacaa acgaatggag atagataaac atagcctaaa
     2941 tattggtgat tacaatcgaa cggtcgggaa aggccctggt tctcggcctc agatttccaa
     3001 agagtcttcc atggagcgca atccttattt tgataaggat ggcattgtag cagatgaatc
     3061 ccaaaacatg cagtttatgt ccagtcaaag catgaagctt cccccttcaa atagtgcact
     3121 acctaaccag gcccttggct ccatagcagg gctgggtatg caaaacttga attctgttag
     3181 acagaatggc aatcccagta tgtttggtgt tggaaacaca gcagcacaac cccggggcat
     3241 gcagcagcct ccagcacaac ctcttagttc atctcagcct aatctccgtg ctcaagtgcc
     3301 tcctccatta ctctcccctc aggttccagt ttcattgctg aagtatgcac caaacaacgg
     3361 tggcctgaat ccactctttg gccctcaaca ggtagccatg ctgaaccagc tatcccagct
     3421 aaaccagctt tctcagatct cccagttaca gcgattgtta gcgcagcagc aaagggcgca
     3481 gagtcagaga agcgtgcctt ctgggaaccg gccgcagcaa gaccagcagg gtcgacctct
     3541 tagtgtgcag cagcaaatga tgcaacaatc tcgtcaactt gatccaaacc tgttggtgaa
     3601 gcagcagact ccaccatctc agcagcagcc actccatcag ccagccatga agtctttcct
     3661 tgacaatgtc atgccccaca ctacacctga gctgcaaaaa gggccatcac caataaatgc
     3721 tttcagcaac ttccctatag gcttgaactc aaacttgaat gtaaatatgg atatgaacag
     3781 tattaaagag ccacagtcaa gactaaggaa gtggacgaca gtggacagca tttctgtgaa
     3841 cacatctttg gatcaaaact ccagcaaaca tggtgctatt tcaagtggtt tcaggctgga
     3901 agagtctcca tttgttccct atgactttat gaacagcagt acttcaccag ccagtcctcc
     3961 aggttcaata ggagatggct ggccacgtgc caaatcgcct aacggctcta gcagtgttaa
     4021 ttggccacca gaatttcgtc ctggtgagcc atggaaaggt tatccaaaca ttgaccctga
     4081 aactgaccct tacgtcactc ctggcagtgt cataaacaat ctttcaatta atactgtgcg
     4141 ggaagttgac cacctcaggg acaggaacag tgggtcatcc tcatccttga acaccacgct
     4201 gccttcaact agtgcctggt catccattcg tgcctccaac tacaacgttc ccctcagcag
     4261 tacagcacaa agcacttcag ccagaaatag tgattccaaa ttgacatggt ctcctggttc
     4321 agttacaaac acctctctgg ctcatgagct gtggaaggtc cctttgccac ctaaaaacat
     4381 cactgctccg tcccgcccac ctccgggact gactggtcag aagccaccct tgtctacgtg
     4441 ggataattct ccccttcgta taggtggagg atggggaaat tctgacgcca gatatacccc
     4501 aggttccagc tggggtgaga gcagctcagg gagaataaca aattggcttg ttctaaaaaa
     4561 ccttacacct cagatcgatg gctcaactct gcgcactctg tgcatgcagc acggcccgct
     4621 gatcacattc cacctgaacc tccctcacgg aaatgctctg gtccgctaca gttcaaaaga
     4681 agaggtagtg gaggcacaaa agtctctgca catgtgtgta ctggggaaca ctactattct
     4741 tgctgagttt gccagtgaag aggagatcag tcgtttcttt gcacaaagcc agtctctgac
     4801 cccttctccc ggctggcagt ctctcgggtc cagccagagc cggctgggct ccctcgactg
     4861 ttcccactca ttctccagcc ggaccgatct caatcactgg aatggtgctg ggctgtcggg
     4921 aactaactgt ggagaccttc acggcacttc actctggggg accccgcatt attccacaag
     4981 cctgtggggt cccccaagca gcagcgaccc ccgaggaatt agcagcccat ctcccattaa
     5041 cgcttttctt tctgttgacc acctgggtgg gggtggagag tccatgtaat ctagagggcc
     5101 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     5161 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     5221 aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg
     5281 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5341 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5401 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     5461 acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc
     5521 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     5581 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     5641 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     5701 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
     5761 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
     5821 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     5881 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     5941 ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6181 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     6241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6421 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7081 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7381 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7741 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     7801 aataggcgta tcacgaggcc ctttcgt
//
