LOCUS       sequence-288720-        8624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot4_F
FEATURES             Location/Qualifiers
     source          1..8624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5909..5950
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5960..5977
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6000..6017)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6050..6184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6074..6093)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6128..6147
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6806..7394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6886..6905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7565..8356,8357..8425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8188..8207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8426..8530)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8598..8616
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgaacggcct
     2761 gagcagcaat gatgatgcag tcgagtgccc tttgtgtatg gagccgcttg aggtggacga
     2821 tctgaccttc tttccgtgta cctgcggata tcagatttgc cgattctgtt ggcataggat
     2881 ccgcacggat gagaacaagc tgtgtccggc atgccgaaag gagtatcccg agaatccagc
     2941 tgacttcaag ccgctgtcgc aggaggaaat gattgccttc aagtcccaga aacgccaaag
     3001 ggaccaacaa cgaaaacaaa agatcaccga gaaccgcaaa cacttggcca acgttcgcgt
     3061 tgtccaaaag aacttggtgt tcgttgtggg gctaccgcca cgacttgctg atgcagacat
     3121 attaaagaaa cacgagtatt tcggtaaata tgggaaaatt cataaagtcg ttataaatcc
     3181 aagtaccacg tatgccgggg tccaggtaag agttggtcca tctgcttctg cgtatgtcac
     3241 atatgttaat aactcggatg ccttgcgggc cattcaaagc gtcaataata ttatgataga
     3301 cggtcggctt ataaagacca gcttggggac aaccaaatac tgtagccact tcatgaagaa
     3361 ccagcagtgt cccaagggcg actgcatgta cttacatgaa ttgggcgatc ccgaggccag
     3421 tttcacaaag gaggaaatgc atcagggaaa gcacctggag tacgagaagc gcctgcacga
     3481 tactctaatt gcgtcattgg gaccgaatgc aacagtcgcg attccatcgt catcgtcagc
     3541 gtcatcctcg tcctctggat cgggtacaaa tggttcaagt gcatcaggta atgcccagca
     3601 aaaggaggcc tggccaagct tatcggtctc gcccatcaat ggcaaagagg cggctgccac
     3661 tgcgaccagt tccagtggga aaagtaaacg ggaaaagctg cgcaacgaga agaggcacga
     3721 gaagaacaag gcgaagaata agaacggcag caatacaaac gccaatgcct cgaacaagga
     3781 gaactatgtt cccgaaacga gaagcagcac aagtactgag acattcgcag aggctacggc
     3841 ggatgcaccg gcttccacca aggcagaacc gccgcaagcc tctagcaata gaacgagggc
     3901 ggatcgtgga aaagatcgga ccacggctag tgcaaaggag cagaagaaga gcaaggaagc
     3961 tgctccagca cctgcagcaa gtaaaccggc ggagcgggtt gaaacaagcg agagtacaat
     4021 aagacaaaag aaggcggaag taacagaaag ctgtgaagat aacttaccac aaaagagatt
     4081 agcgggaaca aacgttcaaa gatctgtgag ctcttgtagc gaaaatagcg aaggacacgt
     4141 ctctgagagt agcttaagtg agaagagttt aactggtgat tatgtggagg aaaagtgcaa
     4201 tagtgtgaat tcggaaagcc agcaagaaag tgtaaagttt caggaggagc tcgaaaagag
     4261 caacgaggca attgtcgagg ccgaaacgat tctgccaaca gctgagtcct ctgaggacat
     4321 cagtcccgct gcagttgcgc caggcaatgg agaagtcgaa ggatgcttac ccgttgttga
     4381 tccagtcgaa ccaccgagtc tggcggataa tggaagcaga gtaacagatg cgctatctaa
     4441 gctgaacatt ttcgatgaca cgcctagctt tttcacatcg ccatcattcc agcaagcacc
     4501 catattaaag aataagctag atttggaaat gcgacagtct catttacctg acttggtcaa
     4561 cgacattgat ggaatccaaa aggcatccaa tacaaacgag tgggaggaag ccttcaaaaa
     4621 tgtgatgatg ggcaacacgc agcacatgga ggagcaactg ttgcagcagc aacatttgca
     4681 gcagcatcag aatttgcgcc accagctggt gttgcaacag gaggagttcc ttcgcatgca
     4741 agaattacag aaacgcaaca actttgcgac gcaaatcaac ggtcctgcga atgatttcct
     4801 tagagcttat gagctcagag ctcaagccaa tgcaatcatt cagcaacaat tattacaaca
     4861 gcacgcaggc gaaaatctat ttggcggcaa catgtcgaag ttcttcgatt tccataaaag
     4921 tcagccgcag tcacaccatc agtatctaaa tggacatccg cctcaaatca atgggaacgg
     4981 tgctgtgccg gagccgcaaa gagtggcggc ctctttggaa agcaatcgac tgaattcgcc
     5041 ttttgtcgaa aatggactta ttaactcgca gcagcagcag cagcagcagc aacagcaaca
     5101 aaagcagaga atgatgggaa tgtatgaatt tatgcctcca aacacgcaat ctcagcagaa
     5161 tcggtttaca cagaactcga tagtcgacga tgacttagga ttcgacccgt tcgttgagac
     5221 ccaaaaggga cttgctgaac ttatggaaaa tgaggtggtc caaaaacaga gtattaataa
     5281 tgagaacccc ttgccgaagt tgccgccaca gcctcaagtt ccaccccatc cgcaattggt
     5341 ggacaacctg cagcgagctc gcatgccgcc gccaggcttc aatcacgtca acacattggg
     5401 cttgggcggt gcatccagac tgcagctcac cagcaaaata atacccttca tgaacatgcc
     5461 cgtcaatggg gtgggcaaca gcggtgccca gggacagcac caaatgccaa tgggtgtcaa
     5521 ctggaatgcg ccgatgggca tgcaccagaa cccgggacag cccgtgggtg attcgcaatt
     5581 gcagcatcca atggctcaca acaaggttta caacaacagt gattggactt caatggatcc
     5641 ggctatactt tcgtttagac agtattcttc ttttccacaa aaccaaattc ccccacatcc
     5701 tcagcagcaa caggatttat ttttgcagca tttggctcaa cagcaaaatt cccagagtgg
     5761 tggtttcaac aaccaggcac agcaaatgct tcctatgggg atgcccaata gtttgctgaa
     5821 cggacaacaa acgcagccgc cacaggtcaa tgccaatgtt cagggcatgc ttgagttttt
     5881 aaaaagccgt caattcgttt agttcgaagg taagcctatc cctaaccctc tcctcggtct
     5941 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     6001 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     6061 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     6121 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     6181 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     6241 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga
     6301 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     6361 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     6421 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     6481 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     6541 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     6601 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     6661 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     6721 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag gccaggaacc
     6781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     7021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7861 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8161 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     8221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     8581 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
