LOCUS       sequence-288738-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGe1-S1274RQ1277R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Jinek M, Eulalio A, Lingel A, Helms S, Conti E, Izaurralde E
  TITLE     The C-terminal region of Ge-1 presents conserved structural features
            required for P-body localization.
  JOURNAL   RNA. 2008 Oct . 14(10):1991-8.
  PUBMED    18755833
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Oct . 14(10):1991-8."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGe1-S1274RQ1277R-V5His6_F
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4566..4575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6839..6880
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6890..6907
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6930..6947)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6980..7114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7004..7023)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7058..7077
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7736..8324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7816..7835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8495..9286,9287..9355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9118..9137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9356..9460)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9528..9546
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgcc gcatgttaat
     2761 cgcgctcttc gcgcttgcac acttgccaat ctttcgtaag acgcctttgt cagcctcctc
     2821 ctccccgccg ccgtctgtcc accggtcgcc aaggtgtggc aaagcaagta ccgcgtacca
     2881 tccaccgcca gcagacgcct gcatcagcat tactgcttca gccattgacg cagcatccgg
     2941 gatgagcaac agcgccgaac aggacaagcc gattgccgcc aacggcaatg cggcagagcc
     3001 ccaggaaata gaagtcatcc atttccaagc gcaagagaag cagtgttgcc gcgttattag
     3061 atccaataag accaaggtcc tcacatccgg cggcgttcac acacgcggtt cctccaaggt
     3121 caagctaaag aacatcgtcg actacaagtg ggagcgcaag tactactatc cgggtcacct
     3181 ggtcgctgtc caccgcgatg gcaagcacct ggcgtacgcc attaatgtca acaacaaagc
     3241 caccggaatg gagggcatgg tgcgcgtatg caacatagcc accagtatgc gagccttgat
     3301 caagggaatg agcggcgagg tcttagatct gcagtttgcc catacggatt gtgagcgtat
     3361 attagctgtg atcgatgtca gttcgctgtt tgtctacaaa gtcgatcaaa tcgaagggaa
     3421 cctgctgtgc aatctagtgc taaaggtgga ggaccccatc gccaactatg tgccggagta
     3481 cgatatggtc tcctggtgtc cctatgtctg cagcagcagc gccaccgttc caatcaacga
     3541 cgatgacgat gagaatcagc tgctcatttg gtcgcgaagc tctcaatttc aatgctttca
     3601 agtgaagatg attgtctctg aacatgggcg cggcaagatc caaccagcag ccctagaatc
     3661 tggatatctg aagattgagg aggactcgtt gatcacgtgc gccgctttgt ccccggatgg
     3721 caccactgtg gctgctgctt gcgccgatgg cttggttcgt ttctaccaaa tttatttgtt
     3781 cgatgtgcgc aaccatcgct gcctgcacga gtggaagcca catgatggca aaaaagtctg
     3841 ctctctcttt tttctcgaca atatcaacaa gcctgtggaa gaatcctact ggcagcacgt
     3901 gatcactacg agcgatgcca ataccgaaat caagctctgg aactgctcgc tatggaaatg
     3961 cctccaaact atcaatgtgg tggccagtcc gtcatctttg cagcccagaa actttatcgc
     4021 gggcatagat cgcagtgcga actacttggt gttgtcttgc ctggactctc tggccgtgta
     4081 tgtaatgcag attggcagta ctggcggcgc agactccgag aataggagta gcgatagcga
     4141 aggagaaggc tgcgacactt ccaaacgcat tcagaatgtt gccgaattca agctgagttc
     4201 ggggattctc tctttttcta ttgtaaacgc tagtatgcgt cgcgttaaga actccatcga
     4261 gagctactat cctattgagg agcccgatga cttcgatgac gattccaatt ccaccagtgc
     4321 ccttgtgctg cacatgtttg tggtgcaggc aaagagcctg caggaatgtc aaataatcta
     4381 ccagccatgt gtggctgaaa agacggagcg gagcagccta aacagcaagc gatcacagac
     4441 gccagaagac aatctgctaa tcaaagagga gccggaatcc cccaacagtg gaactgtggg
     4501 tgctgttcag ctggatgcat tattcgcaaa atccgcaaag agagcttcaa cgggcagttc
     4561 atctgccatg gtggccgtgg cggcagccgc ggctgcggca ccttcggcca tcctgcagga
     4621 cgccactaag gaggcggcca agtcagagtc cccgcaacta agcagcgcgt atacgcagca
     4681 ggttaatctc atgactccag atgccttcag tgcaagcgga accgcagcgg cagcagctgt
     4741 atttgtgtct accagcacca caacttcaat aggaactgat agctctacca ccaccagcgg
     4801 tcaggataga tccattgatt cagcggttct gcaaactata cgaatgctgg ccacggtaac
     4861 gtccaagact tccgaaaacc ccaatgccga ggtactcctc aacctaatga acaacaccct
     4921 aatcgaagat cgcgagcaac aaaagctgaa ggaaaagctt gacgctcgca agaagttcat
     4981 tgcaattgat cgcaatccag agcggaatgt ggctgagaac ttggccagcg gcagttcgag
     5041 tccgagtcga gaggtgcagg agataatggc cacacaggac gatgccgatg cctacgaagc
     5101 agagctagag aatctggacg acgatgatga cgatgaggaa gaagagctgg caaattcatc
     5161 accgctcccg gaagctgttg acggaacatg gcccattgtc aagctgtcat cccactcagc
     5221 tgagctccag aatgctgccc agatcatgtc ccaggcggtg cagaacacca acaatggcaa
     5281 tgtgccgcct acgctaggtg gagggcacaa taataacaca agtgtaggca gcaatagcaa
     5341 caacaacacg gctacaactc ttagcaccag caacaccagc agcagcaaca acgcgggcgg
     5401 aacatgcgtt gattcaagtg gaactggaga gctaaatgcc aaaatggaac tactcataga
     5461 tctagtaaaa gcccagtcca aacaaataaa caaattggag aacgaagtca acaagctaca
     5521 aaagcagcaa gaagcggcgg cggccctgca tagcaaacaa gacacttcat tggagcctaa
     5581 aaatcttagt cagctagcat ataaaattga gatgcaactg tccaagctaa tggagcagta
     5641 tctcaagcga tatgaaaacg agcataagaa gaaactgacg gaattcttgg cggcgcgtga
     5701 gagccaaaac cgagagctac gtgactccgt gctgcaggta ctaaatcagt atgtcatgaa
     5761 ccactttacg gacattattg gcaacgttct caacatggaa ctgcagcgcc aactgcttcc
     5821 tcgcgtgaat gccaacatgg atcagctcca agcccaaatg caagtggaga ttgtgcagaa
     5881 gctgagtgtg tttgacaaaa cggtgaagga gaatattgcc caggtgtgca agagcaagca
     5941 atttctggat acttttggca agtccgtgtt gattggtgtt caaaccagcc tacaaaccgc
     6001 cttcattgaa tctatgagca gcaccttgat tccagcttat gagaagtcct cgcagaacat
     6061 gtttaaacaa ctgcatgacg ctttcagcgt gggcataaaa gactttatgg tccagtttaa
     6121 cacctacctg cagcacatgc cacagcccca agccgggagt ggcaatacgg aggagataaa
     6181 caacaagttg tccatgctta agcaattggt agagagcagc ttgcacaagc accgcactga
     6241 actcacggac gccatgctcg agacacaaag agaagtgaag agcttagaga ttttgctggc
     6301 gcgtcaggta caggaaacga tcagagcaga gctacgcaag cacatggaag cccagaacgt
     6361 ggcaatgcgt tcgcaggcag ctacgcctgc accaccttac gacctcaggg acagcattaa
     6421 gcagcttttg atggctggac aaatcaacaa ggccttccat caggctctat tggccaacga
     6481 cttgggccta gtggagttca ctctccgaca cacggacagc aaccaggcat tcgcaccaga
     6541 gggatgcaga ctggagcaaa aggttctact gcgtctcatt cggcaaatat ccgctgacat
     6601 gaccaatcac aatgagctta agcagagata tctcaacgag gcactgttgg ccatcaatat
     6661 ggcagatccc attacccgcg agcacgctcc taaagtgctc accgagctgt atcgcaattg
     6721 tcagcagttc attaagaaca gccccaagaa ttcgcagttc agcaacgtcc gtctgctgat
     6781 gaaggcaatc ataacgtacc gcgatcagct aaaaagtcta gagggcccgc ggttcgaagg
     6841 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     6901 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg
     6961 ctgatcagcc tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac
     7021 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7081 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7141 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     7201 tggtatggct gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct
     7261 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     7321 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     7381 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     7441 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     7501 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     7561 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     7621 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     7681 ctcttaccag ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7921 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8161 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8401 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     8821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9061 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     9121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9481 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9541 cgaggccctt tcgt
//
