LOCUS       sequence-288740-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6A-delAGOhook.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A-delAGOhook_F
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5354..5365
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6327..6346)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6381..6400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6619..7074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6706..6725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6916..6937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7191..7548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7191..7382
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7214..7234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7399..7534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7599..8393
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7653..7672)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8263..8282
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8457..8505
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8546..8565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8665..8687
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8725..8743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8811..8915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8916..8984,8985..9776)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9134..9153)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9947..10535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10436..10455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggcg
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acagtggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattgta aacagaactg
     2581 acttagatcc acgtgtcctg tccaactctg gttggggaca gactcctatt aagcagaata
     2641 ctgcctggga tacagaaaca tcacctagag gggaacgaaa gactgacaat gggacagagg
     2701 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2761 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2821 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2881 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2941 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     3001 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     3061 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3121 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3181 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3241 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3301 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3361 ggggacctat accagcccca gcaaaagaag aagaacccac aggctgggag gaaccatccc
     3421 cagaatctat acgtcgcaaa atggagattg atgatggaac ttcagcttgg ggagatccaa
     3481 gcaaatacaa ctacaaaaat gtgaacatgt ggaacaaaaa cgtcccaaat ggcaacagcc
     3541 gttcagacca gcaagcacag gtacatcagc tgctaacgcc tgcaagtgcc atctcaaaca
     3601 aagaggcaag cagtggctct ggctggggtg agccctgggg ggagccttct actccagcca
     3661 caactgctgc ggcatccagc acatccacgt ggggctccag ctctgttggt ccacaagcat
     3721 taagcaaatc tgggccaaaa tctatgcaag atggctggtg tggtgatgat atgccattgc
     3781 ctggaaatcg ccccactggc tgggaagagg aagaggatgt ggagattgga atgtggaata
     3841 gtaattcatc tcaagagctt aactcatctt taaattggcc accatataca aagaaaatgt
     3901 catcgaaggg tctgagtggc aaaaaaagga gaagggaaag gggaatgatg aaaggtggaa
     3961 acaaacaaga agaagcgtgg ataaatccat ttgttaaaca gttttcaaac atcagttttt
     4021 cgagagactc accagaggaa aatgtacaaa gcaataagat ggacctttct ggaggaatgt
     4081 tacaagacaa acgaatggag atagataaac atagcctaaa tattggtgat tacaatcgaa
     4141 cggtcgggaa aggccctggt tctcggcctc agatttccaa agagtcttcc atggagcgca
     4201 atccttattt tgataaggat ggcattgtag cagatgaatc ccaaaacatg cagtttatgt
     4261 ccagtcaaag catgaagctt cccccttcaa atagtgcact acctaaccag gcccttggct
     4321 ccatagcagg gctgggtatg caaaacttga attctgttag acagaatggc aatcccagta
     4381 tgtttggtgt tggaaacaca gcagcacaac cccggggcat gcagcagcct ccagcacaac
     4441 ctcttagttc atctcagcct aatctccgtg ctcaagtgcc tcctccatta ctctcccctc
     4501 aggttccagt ttcattgctg aagtatgcac caaacaacgg tggcctgaat ccactctttg
     4561 gccctcaaca ggtagccatg ctgaaccagc tatcccagct aaaccagctt tctcagatct
     4621 cccagttaca gcgattgtta gcgcagcagc aaagggcgca gagtcagaga agcgtgcctt
     4681 ctgggaaccg gccgcagcaa gaccagcagg gtcgacctct tagtgtgcag cagcaaatga
     4741 tgcaacaatc tcgtcaactt gatccaaacc tgttggtgaa gcagcagact ccaccatctc
     4801 agcagcagcc actccatcag ccagccatga agtctttcct tgacaatgtc atgccccaca
     4861 ctacacctga gctgcaaaaa gggccatcac caataaatgc tttcagcaac ttccctatag
     4921 gcttgaactc aaacttgaat gtaaatatgg atatgaacag tattaaagag ccacagtcaa
     4981 gactaaggaa gtggacgaca gtggacagca tttctgtgaa cacatctttg gatcaaaact
     5041 ccagcaaaca tggtgctatt tcaagtggtt tcaggctgga agagtctcca tttgttccct
     5101 atgactttat gaacagcagt acttcaccag ccagtcctcc aggttcaata ggagatggct
     5161 ggccacgtgc caaatcgcct aacggctcta gcagtgttaa ttggccacca gaatttcgtc
     5221 ctggtgagcc atggaaaggt tatccaaaca ttgaccctga aactgaccct tacgtcactc
     5281 ctggcagtgt cataaacaat ctttcaatta atactgtgcg ggaagttgac cacctcaggg
     5341 acaggaacag tgggtcatcc tcatccttga acaccacgct gccttcaact agtgcctggt
     5401 catccattcg tgcctccaac tacaacgttc ccctcagcag tacagcacaa agcacttcag
     5461 ccagaaatag tgattccaaa ttgacatggt ctcctggttc agttacaaac acctctctgg
     5521 ctcatgagct gtggaaggtc cctttgccac ctaaaaacat cactgctccg tcccgcccac
     5581 ctccgggact gactggtcag aagccaccct tgtctacgtg ggataattct ccccttcgta
     5641 taggtggagg atggggaaat tctgacgcca gatatacccc aggttccagc tggggtgaga
     5701 gcagctcagg gagaataaca aattggcttg ttctaaaaaa ccttacacct cagatcgatg
     5761 gctcaactct gcgcactctg tgcatgcagc acggcccgct gatcacattc cacctgaacc
     5821 tccctcacgg aaatgctctg gtccgctaca gttcaaaaga agaggtagtg gaggcacaaa
     5881 agtctctgca catgtgtgta ctggggaaca ctactattct tgctgagttt gccagtgaag
     5941 aggagatcag tcgtttcttt gcacaaagcc agtctctgac cccttctccc ggctggcagt
     6001 ctctcgggtc cagccagagc cggctgggct ccctcgactg ttcccactca ttctccagcc
     6061 ggaccgatct caatcactgg aatggtgctg ggctgtcggg aactaactgt ggagaccttc
     6121 acggcacttc actctggggg accccgcatt attccacaag cctgtggggt cccccaagca
     6181 gcagcgaccc ccgaggaatt agcagcccat ctcccattaa cgcttttctt tctgttgacc
     6241 acctgggtgg gggtggagag tccatgtaag cggccgcttc cctttagtga gggttaatgc
     6301 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     6361 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     6421 gctgcaataa acaagtaaca acaattgcat tcattttatg tttcaggttc agggggagat
     6481 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc gataaggatc
     6541 gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6601 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6961 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7021 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
     7081 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     7141 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     7201 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7261 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7321 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct
     7561 tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa gatggattgc
     7621 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7681 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7741 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     7801 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7861 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     7921 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     7981 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     8041 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     8101 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     8161 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8221 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8281 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8341 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     8401 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata
     8461 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa
     8521 ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     8581 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8641 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     8701 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     8761 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8881 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8941 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9001 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9061 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9121 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9181 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9241 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9301 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9361 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9421 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9481 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9541 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9601 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9661 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9721 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9781 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9841 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9961 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10021 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10081 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10141 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10201 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10261 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10321 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10381 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10441 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10501 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10561 ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct
//
