LOCUS       sequence-288741-       10554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6A-delMI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A-delMI_F
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5300..5311
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6273..6292)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6327..6346
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6565..7020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6652..6671)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6862..6883
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7137..7494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7137..7328
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7160..7180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7345..7480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7545..8339
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7599..7618)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8209..8228
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8403..8451
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8492..8511)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8611..8633
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8671..8689)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8757..8861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8862..8930,8931..9722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9080..9099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9893..10481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10382..10401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggcg
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acagtggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattaat gggacagagg
     2581 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2641 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2701 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2761 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2821 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     2881 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     2941 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3001 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3061 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3121 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3181 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3241 ggggacctat accagcccca gcaaaagaag aagaacccac aggctgggag gaaccatccc
     3301 cagaatctat acgtcgcaaa atggagattg atgatggaac ttcagcttgg ggagatccaa
     3361 gcaaatacaa ctacaaaaat gtgaacatgt ggaacaaaaa cgtcccaaat ggcaacagcc
     3421 gttcagacca gcaagcacag gtacatcagc tgctaacgcc tgcaagtgcc atctcaaaca
     3481 aagaggcaag cagtggctct ggctggggtg agccctgggg ggagccttct actccagcca
     3541 caactgtgga taatggtact tcagcatggg gtaagcccat agacagtggt cccagctggg
     3601 gggaacccat tgctgcggca tccagcacat ccacgtgggg ctccagctct gttggtccac
     3661 aagcattaag caaatctggg ccaaaatcta tgcaagatgg ctggtgtggt gatgatatgc
     3721 cattgcctgg aaatcgcccc actggctggg aagaggaaga ggatgtggag attggaatgt
     3781 ggaatagtaa ttcatctcaa gagcttaact catctttaaa ttggccacca tatacaaaga
     3841 aaatgtcatc gaagggtctg agtggcaaaa aaaggagaag ggaaagggga atgatgaaag
     3901 gtggaaacaa acaagaagaa gcgtggataa atccatttgt taaacagttt tcaaacatca
     3961 gtttttcgag agactcacca gaggaaaatg tacaaagcaa taagatggac ctttctggag
     4021 gaatgttaca agacaaacga atggagatag ataaacatag cctaaatatt ggtgattaca
     4081 atcgaacggt cgggaaaggc cctggttctc ggcctcagat ttccaaagag tcttccatgg
     4141 agcgcaatcc ttattttgat aaggatggca ttgtagcaga tgaatcccaa aacatgcagt
     4201 ttatgtccag tcaaagcatg aagcttcccc cttcaaatag tgcactacct aaccaggccc
     4261 ttggctccat agcagggctg ggtatgcaaa acttgaattc tgttagacag aatggcaatc
     4321 ccagtatgtt tggtgttgga aacacagcag cacaaccccg gggcatgcag cagcctccag
     4381 cacaacctct tagttcatct cagcctaatc tccgtgctca agtgcctcct ccattactct
     4441 cccctcaggt tccagtttca ttgctgaagt atgcaccaaa caacggtggc ctgaatccac
     4501 tctttggccc tcaacaggta gccatgctga accagctatc ccagctaaac cagctttctc
     4561 agatctccca gttacagcga ttgttagcgc agcagcaaag ggcgcagagt cagagaagcg
     4621 tgccttctgg gaaccggccg cagcaagacc agcagggtcg acctcttagt gtgcagcagc
     4681 aaatgatgca acaatctcgt caacttgatc caaacctgtt ggtgaagcag cagactccac
     4741 catctcagca gcagccactc catcagccag ccatgaagtc tttccttgac aatgtcatgc
     4801 cccacactac acctgagctg caaaaagggc catcaccaat aaatgctttc agcaacttcc
     4861 ctataggctt gaactcaaac ttgaatgtaa atatggatat gaacagtatt aaagagccac
     4921 agtcaagact aaggaagtgg acgacagtgg acagcatttc tgtgaacaca tctttggatc
     4981 aaaactccag caaacatggt gctatttcaa gtggtttcag gctggaagag tctccatttg
     5041 ttccctatga ctttatgaac agcagtactt caccagccag tcctccaggt tcaataggag
     5101 atggctggcc acgtgccaaa tcgcctaacg gctctagcag tgttaattgg ccaccagaat
     5161 ttcgtcctgg tgagccatgg aaaggttatc caaacattga ccctgaaact gacccttacg
     5221 tcactcctgg cagtgtcata aacaatcttt caattaatac tgtgcgggaa gttgaccacc
     5281 tcagggacag gaacagtggg tcatcctcat ccttgaacac cacgctgcct tcaactagtg
     5341 cctggtcatc cattcgtgcc tccaactaca acgttcccct cagcagtaca gcacaaagca
     5401 cttcagccag aaatagtgat tccaaattga catggtctcc tggttcagtt acaaacacct
     5461 ctctggctca tgagctgtgg aaggtccctt tgccacctaa aaacatcact gctccgtccc
     5521 gcccacctcc gggactgact ggtcagaagc cacccttgtc tacgtgggat aattctcccc
     5581 ttcgtatagg tggaggatgg ggaaattctg acgccagata taccccaggt tccagctggg
     5641 gtgagagcag ctcagggaga ataacaaatt ggcttgttct aaaaaacctt acacctcaga
     5701 tcgatggctc aactctgcgc actctgtgca tgcagcacgg cccgctgatc acattccacc
     5761 tgaacctccc tcacggaaat gctctggtcc gctacagttc aaaagaagag gtagtggagg
     5821 cacaaaagtc tctgcacatg tgtgtactgg ggaacactac tattcttgct gagtttgcca
     5881 gtgaagagga gatcagtcgt ttctttgcac aaagccagtc tctgacccct tctcccggct
     5941 ggcagtctct cgggtccagc cagagccggc tgggctccct cgactgttcc cactcattct
     6001 ccagccggac cgatctcaat cactggaatg gtgctgggct gtcgggaact aactgtggag
     6061 accttcacgg cacttcactc tgggggaccc cgcattattc cacaagcctg tggggtcccc
     6121 caagcagcag cgacccccga ggaattagca gcccatctcc cattaacgct tttctttctg
     6181 ttgaccacct gggtgggggt ggagagtcca tgtaagcggc cgcttccctt tagtgagggt
     6241 taatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     6301 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6361 ttataagctg caataaacaa gtaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6421 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatccgata
     6481 aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6541 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6601 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6661 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6721 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6781 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6841 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6901 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6961 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7021 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgcggatc
     7081 tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     7141 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7321 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7381 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     7501 gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt gaacaagatg
     7561 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7621 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7681 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     7741 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7801 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7861 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7921 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7981 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     8041 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     8101 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     8161 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     8221 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     8281 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     8341 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc
     8401 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     8461 cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8521 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8581 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8641 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8701 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8761 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8821 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8881 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8941 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9001 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9061 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9121 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9181 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9241 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9301 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9361 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9421 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9481 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9541 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9601 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9661 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9721 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9781 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9841 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9961 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10021 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10501 ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag atct
//
