LOCUS       sequence-288747-       10624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6Biso1-delMI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI_F
FEATURES             Location/Qualifiers
     source          1..10624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6328..6449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6339..6358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6393..6412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6635..7090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6722..6741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6932..6953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7207..7564
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7207..7398
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7230..7250)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7415..7550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7615..8409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7669..7688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8279..8298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8473..8521
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8562..8581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8681..8703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8741..8759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8827..8931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8932..9000,9001..9792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9150..9169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9963..10551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10452..10471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcacg cgtggtacct ctagagatgg
     1201 cctacccata tgatgttcca gattacgcta tgagagagaa ggagcaagaa agggaagaac
     1261 agttaatgga agacaagaaa aggaagaaag aggataaaaa gaaaaaagaa gccactcaga
     1321 aggtcacgga acaaaaaacc aaagtgcccg aagtgacgaa accaagttta agccaaccaa
     1381 cggccgccag cccaattggc agctctccat cgccaccagt caatggtggc aacaatgcca
     1441 aaagggtggc agtgccgaac ggacaaccgc caagcgccgc ccgctacatg cctcgggagg
     1501 tgccgccgcg attccgttgc cagcaggacc acaaagtgtt actaaaacgt gggcagcccc
     1561 ctccaccgtc ctgcatgctc cttgggggtg gggcagggcc tcctccctgc acagcacctg
     1621 gagcaaaccc aaacaacgca caagtgacag gagcgctgct gcagagtgag agtgggactg
     1681 cgccagactc aacccttgga ggtgctgctg cttcaaatta tgcaaattcc acttggggct
     1741 cgggagcctc ctccaacaac ggcacctccc ccaacccaat tcacatctgg gacaaggtga
     1801 ttgtagacgg gtctgacatg gaagagtggc cttgtattgc cagcaaagac actgaatctt
     1861 cttccgaaaa caccaccgat aacaacagtg cctcgaaccc tggctctgag aagagcactc
     1921 tgccaggaag caccactagt aacaaaggaa aagggagcca gtgccagtct gcaagttctg
     1981 ggaacgaatg taatcttggg gtctggaaat ctgaccctaa ggctaaatct gttcaatctt
     2041 ccaactctac tacagagaac aacaatggac taggaaattg gaggaatgtg agtggtcagg
     2101 atagaattgg acctggctct ggcttcagca actttaaccc aaatagcaac ccatctgcct
     2161 ggccagcact ggtccaagaa ggaacttcta ggaaaggggc attggaaaca gataatagta
     2221 attccagtgc acaggttagc acagtaggtc agacatccag ggaacagcag tcaaagatgg
     2281 aaaatgcggg tgttaatttt gttgtctctg gcagagaaca ggctcaaatt cataacactg
     2341 atggaccaaa aaatggaaac actaactcct tgaacttaag ttcaccaaac cccatggaga
     2401 ataagggaat gccctttgga atgggcttgg ggaacacctc caggagcact gatgcccctt
     2461 cacaaagcac tggagatcga aagactggga gtgttggatc ttggggtgca gctagggggc
     2521 cttctggaac tgacacagtc tctggacaaa gcaattctgg aaacaatggg aacaatggaa
     2581 aagagagaga ggactcctgg aaaggagctt ctgttcagaa atcaactggg tcaaaaaatg
     2641 actcttggga caacaataac aggtctacgg gtgggtcctg gaactttggc ccccaggact
     2701 ctaatgacaa caaatggggt gaagggaaca aaatgacatc tggggtctct cagggagaat
     2761 ggaaacagcc gactgggtct gatgagttga aaattggaga atggagtggt ccaaaccaac
     2821 caaattctag cactggagca tgggacaatc aaaagggcca ccccctccct gaaaaccaag
     2881 gcaatgccca ggctccctgt tggggaagat cttccagctc cacaggaagt gaagttggag
     2941 gtcaaagcac tggaagcaac cacaaagcag gaagtagtga cagtcataac tctggccgtc
     3001 ggtcgtacag gcccacacat cctgattgtc aggctgtctt gcagactctt aaaggaactg
     3061 aggggtggga gagcgctgcc acacagacca agaactcagg gggctgggga gatgcaccca
     3121 gccaaagcaa tcaaatgaag tctggatggg gggagctctc agcctctaca gagtggaaag
     3181 accccaagaa cacaggaggc tggaatgact acaagaacaa caactcttcc aactggggag
     3241 gaggacgacc tgatgaaaag acaccttcct cttggaatga gaatcccagc aaggatcagg
     3301 ggtggggagg tggacgccag cccaatcaag gatggtcttc tggaaagaat ggttgggggg
     3361 aggaagtcga tcagacaaaa aacagcaatt gggaaagttc tgcaagtaaa cctgtgtctg
     3421 ggtggggtga aggagggcag aatgaaatcg ggacttgggg taatggtggc aatgcaagcc
     3481 tagcttcaaa aggtgggtgg gaggattgca aaagatcccc agcatggaat gagacgggcc
     3541 gacagcccaa ttcctggaat aaacaacacc aacagcagca gcccccacag cagccgccgc
     3601 caccacaacc agaggcttct ggttcgtggg gaggcccacc cccaccacct ccaggcaacg
     3661 ttcgaccttc caattccagc tggagcagcg ggccacagcc tgcaacacct aaggatgagg
     3721 aacccagtgg ttgggaagag ccatccccac agtcaattag tcggaaaatg gacattgatg
     3781 atggcacttc agcatgggga gaccctaaca gttataacta caagaatgtg aatctgtggg
     3841 ataagaattc ccaagggggc ccagcacctc gagaaccaaa cctgcccacc ccaatgacca
     3901 gtaaatcggc atcagattcc aaatctatgc aagacggctg gggggagagt gacgggccag
     3961 tcacaggagc tcgccatccc agctgggaag aggaggagga tggaggagtc tggaacacca
     4021 ctggctctca gggcagtgct tcctcccaca actcagcaag ctggggacaa ggaggaaaga
     4081 aacaaatgaa gtgctcactc aaaggaggaa acaatgattc atggatgaat cctcttgcca
     4141 aacagttttc aaatatggga ttgctgagtc agactgaaga taatccaagc agcaaaatgg
     4201 atttgtctgt aggaagcctt tcagataaaa aatttgatgt ggacaagcga gcgatgaatc
     4261 tcggggattt taatgatatc atgaggaagg atcgatctgg gttccgtcca cctaattcca
     4321 aagacatggg aaccacagat agtgggcctt attttgagaa gggcggtagt catggtttgt
     4381 ttggaaacag cacagcacaa tcgagaggtc tgcacacacc cgtgcagcca ctaaattctt
     4441 ctcccagtct ccgggcgcaa gtgcctcccc agtttatttc cccccaggtt tctgcctcaa
     4501 tgctcaagca gtttcccaac agtggcctga gtccaggtct tttcaatgtg gggccccagt
     4561 tatctcctca acaaattgcc atgctgagcc agcttccaca aattccccag tttcagttgg
     4621 catgtcagct tctcttgcag cagcagcaac agcagcagtt gttacagaac cagagaaaga
     4681 tttctcaagc tgtacgccaa cagcaagagc agcagctggc tcgaatggtg agtgcactgc
     4741 agcagcagca gcagcagcag cagaggcagc caggcatgaa gcactcgccc tctcatcctg
     4801 ttgggcccaa gccgcatctg gacaacatgg tacccaacgc attgaatgtg gggctcccag
     4861 accttcaaac caaagggcca atacctggat atggttctgg cttcagctct ggcggcatgg
     4921 actatggcat ggttggtggg aaggaggctg gaaccgagtc tcgctttaaa cagtggacct
     4981 ccatgatgga ggggctgccc tctgtagcca cacaggaagc caatatgcac aaaaatggcg
     5041 ctatagtggc ccctggtaaa acccggggag ggtcaccgta caaccagttt gatatcatcc
     5101 ctggtgacac actgggtggc catacgggtc ctgctggtga tagctggtta cctgccaaat
     5161 ctccaccaac aaataaaatc ggaagtaaat ccagcaatgc cagttggcct ccagaattcc
     5221 aaccaggagt gccatggaaa ggtatccaaa acattgaccc tgaatctgac ccctatgtca
     5281 ccccaggaag tgtgctgggg ggtacagcca catctcccat tgtagatact gaccaccaac
     5341 tgctgcggga taacaccaca gggtctaatt cttccctcaa cacctcgctg ccttcacctg
     5401 gtgcctggcc ctacagtgcc tctgacaact cctttaccaa cgttcatagc acttcagcaa
     5461 agttccctga ttacaaatca acatggtccc cagatcccat aggacacaac cccactcatc
     5521 tctccaacaa gatgtggaaa aaccatattt cctccaggaa cactacaccg ctgccccgcc
     5581 cacctcctgg tctgaccaac cccaaaccat catctccctg gagcagcaca gcaccccgat
     5641 cagtcagggg gtgggggaca caggactcac ggctcgcctc ggcctctacc tggagtgatg
     5701 gtggctcagt tcgtcctagt tactggctgg ttcttcacaa tctcacccca cagattgatg
     5761 ggtcaacctt gagaacgatc tgcatgcagc atggcccact gctgacattc catctgaatc
     5821 taacccaggg cactgccctg atccgataca gcaccaaaca ggaggcggcc aaggcccaaa
     5881 ctgcactgca catgtgtgtg ttgggaaaca ctaccatcct tgctgagttt gccactgatg
     5941 atgaagtcag ccgctttctg gcacaagctc agccccctac acctgcagca accccaagtg
     6001 cgccagctgc ggggtggcag tcgctggaga ccggccagaa ccagtcagat cccgtgggac
     6061 ctgctctgaa tctttttggt gggtccactg ggctcgggca gtggagcagc agcgctggtg
     6121 gcagcagcgg ggccgatctt gctggcgctt cattgtgggg gcccccaaac tattcttcta
     6181 gcttatgggg agtcccaacg gtggaagatc cccataggat gggcagccct gctcctttac
     6241 tacctggtga ccttctggga ggagggtcgg attcaatctg agcggccgct tccctttagt
     6301 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     6361 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6421 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6481 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6541 aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc
     6601 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     6661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6961 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7081 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7141 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     7201 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7261 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7381 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7441 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7501 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7561 caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt
     7621 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7681 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7741 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7801 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7861 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7921 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7981 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8041 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8101 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8161 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8221 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8281 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8341 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8401 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8461 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     8521 gaatcgatag cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc
     8581 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8641 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8701 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8761 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8821 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8881 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8941 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9001 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9061 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9121 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9181 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9241 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9301 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9361 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9421 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9481 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9541 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9601 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9661 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9721 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9781 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9841 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9901 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9961 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10021 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10081 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10141 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10201 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10261 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10321 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10381 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10441 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10501 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10561 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag
    10621 atct
//
