LOCUS       sequence-288749-       10495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6Biso1-delMI-MII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI-MII_F
FEATURES             Location/Qualifiers
     source          1..10495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6199..6320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6210..6229)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6264..6283
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6506..6961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6593..6612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6803..6824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7078..7435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7078..7269
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7101..7121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7286..7421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7486..8280
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7540..7559)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8150..8169
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8344..8392
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8433..8452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8552..8574
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8612..8630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8698..8802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8803..8871,8872..9663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9021..9040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9834..10422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10323..10342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcacg cgtggtacct ctagagatgg
     1201 cctacccata tgatgttcca gattacgcta tgagagagaa ggagcaagaa agggaagaac
     1261 agttaatgga agacaagaaa aggaagaaag aggataaaaa gaaaaaagaa gccactcaga
     1321 aggtcacgga acaaaaaacc aaagtgcccg aagtgacgaa accaagttta agccaaccaa
     1381 cggccgccag cccaattggc agctctccat cgccaccagt caatggtggc aacaatgcca
     1441 aaagggtggc agtgccgaac ggacaaccgc caagcgccgc ccgctacatg cctcgggagg
     1501 tgccgccgcg attccgttgc cagcaggacc acaaagtgtt actaaaacgt gggcagcccc
     1561 ctccaccgtc ctgcatgctc cttgggggtg gggcagggcc tcctccctgc acagcacctg
     1621 gagcaaaccc aaacaacgca caagtgacag gagcgctgct gcagagtgag agtgggactg
     1681 cgccagactc aacccttgga ggtgctgctg cttcaaatta tgcaaattcc acttggggct
     1741 cgggagcctc ctccaacaac ggcacctccc ccaacccaat tcacatctgg gacaaggtga
     1801 ttgtagacgg gtctgacatg gaagagtggc cttgtattgc cagcaaagac actgaatctt
     1861 cttccgaaaa caccaccgat aacaacagtg cctcgaaccc tggctctgag aagagcactc
     1921 tgccaggaag caccactagt aacaaaggaa aagggagcca gtgccagtct gcaagttctg
     1981 ggaacgaatg taatcttggg gtctggaaat ctgaccctaa ggctaaatct gttcaatctt
     2041 ccaactctac tacagagaac aacaatggac taggaaattg gaggaatgtg agtggtcagg
     2101 atagaattgg acctggctct ggcttcagca actttaaccc aaatagcaac ccatctgcct
     2161 ggccagcact ggtccaagaa ggaacttcta ggaaaggggc attggaaaca gataatagta
     2221 attccagtgc acaggttagc acagtaggtc agacatccag ggaacagcag tcaaagatgg
     2281 aaaatgcggg tgttaatttt gttgtctctg gcagagaaca ggctcaaatt cataacactg
     2341 atggaccaaa aaatggaaac actaactcct tgaacttaag ttcaccaaac cccatggaga
     2401 ataagggaat gccctttgga atgggcttgg ggaacacctc caggagcact gatgcccctt
     2461 cacaaagcac tggagatcga aagactggga gtgttggatc ttggggtgca gctagggggc
     2521 cttctggaac tgacacagtc tctggacaaa gcaattctgg aaacaatggg aacaatggaa
     2581 aagagagaga ggactcctgg aaaggagctt ctgttcagaa atcaactggg tcaaaaaatg
     2641 actcttggga caacaataac aggtctacgg gtgggtcctg gaactttggc ccccaggact
     2701 ctaatgacaa caaatggggt gaagggaaca aaatgacatc tggggtctct cagggagaat
     2761 ggaaacagcc gactgggtct gatgagttga aaattggaga atggagtggt ccaaaccaac
     2821 caaattctag cactggagca tgggacaatc aaaagggcca ccccctccct gaaaaccaag
     2881 gcaatgccca ggctccctgt tggggaagat cttccagctc cacaggaagt gaagttggag
     2941 gtcaaagcac tggaagcaac cacaaagcag gaagtagtga cagtcataac tctggccgtc
     3001 ggtcgtacag gcccacacat cctgattgtc aggctgtctt gcagactctt aaaggaactg
     3061 aggggtggga gagcgctgcc acacagacca agaactcagg gggctgggga gatgcaccca
     3121 gccaaagcaa tcaaatgaag tctggatggg gggagctctc agcctctaca gagtggaaag
     3181 accccaagaa cacaggaggc tggaatgact acaagaacaa caactcttcc aactggggag
     3241 gaggacgacc tgatgaaaag acaccttcct cttggaatga gaatcccagc aaggatcagg
     3301 ggtggggagg tggacgccag cccaatcaag gatggtcttc tggaaagaat ggttgggggg
     3361 aggaagtcga tcagacaaaa aacagcaatt gggaaagttc tgcaagtaaa cctgtgtctg
     3421 ggtggggtga aggagggcag aatgaaatcg ggacttgggg taatggtggc aatgcaagcc
     3481 tagcttcaaa aggtgggtgg gaggattgca aaagatcccc agcatggaat gagacgggcc
     3541 gacagcccaa ttcctggaat aaacaacacc aacagcagca gcccccacag cagccgccgc
     3601 caccacaacc agaggcttct ggttcgtggg gaggcccacc cccaccacct ccaggcaacg
     3661 ttcgaccttc caattccagc tggagcagcg ggccacagcc tgcaacacct aaggatgagt
     3721 cccaaggggg cccagcacct cgagaaccaa acctgcccac cccaatgacc agtaaatcgg
     3781 catcagattc caaatctatg caagacggct ggggggagag tgacgggcca gtcacaggag
     3841 ctcgccatcc cagctgggaa gaggaggagg atggaggagt ctggaacacc actggctctc
     3901 agggcagtgc ttcctcccac aactcagcaa gctggggaca aggaggaaag aaacaaatga
     3961 agtgctcact caaaggagga aacaatgatt catggatgaa tcctcttgcc aaacagtttt
     4021 caaatatggg attgctgagt cagactgaag ataatccaag cagcaaaatg gatttgtctg
     4081 taggaagcct ttcagataaa aaatttgatg tggacaagcg agcgatgaat ctcggggatt
     4141 ttaatgatat catgaggaag gatcgatctg ggttccgtcc acctaattcc aaagacatgg
     4201 gaaccacaga tagtgggcct tattttgaga agggcggtag tcatggtttg tttggaaaca
     4261 gcacagcaca atcgagaggt ctgcacacac ccgtgcagcc actaaattct tctcccagtc
     4321 tccgggcgca agtgcctccc cagtttattt ccccccaggt ttctgcctca atgctcaagc
     4381 agtttcccaa cagtggcctg agtccaggtc ttttcaatgt ggggccccag ttatctcctc
     4441 aacaaattgc catgctgagc cagcttccac aaattcccca gtttcagttg gcatgtcagc
     4501 ttctcttgca gcagcagcaa cagcagcagt tgttacagaa ccagagaaag atttctcaag
     4561 ctgtacgcca acagcaagag cagcagctgg ctcgaatggt gagtgcactg cagcagcagc
     4621 agcagcagca gcagaggcag ccaggcatga agcactcgcc ctctcatcct gttgggccca
     4681 agccgcatct ggacaacatg gtacccaacg cattgaatgt ggggctccca gaccttcaaa
     4741 ccaaagggcc aatacctgga tatggttctg gcttcagctc tggcggcatg gactatggca
     4801 tggttggtgg gaaggaggct ggaaccgagt ctcgctttaa acagtggacc tccatgatgg
     4861 aggggctgcc ctctgtagcc acacaggaag ccaatatgca caaaaatggc gctatagtgg
     4921 cccctggtaa aacccgggga gggtcaccgt acaaccagtt tgatatcatc cctggtgaca
     4981 cactgggtgg ccatacgggt cctgctggtg atagctggtt acctgccaaa tctccaccaa
     5041 caaataaaat cggaagtaaa tccagcaatg ccagttggcc tccagaattc caaccaggag
     5101 tgccatggaa aggtatccaa aacattgacc ctgaatctga cccctatgtc accccaggaa
     5161 gtgtgctggg gggtacagcc acatctccca ttgtagatac tgaccaccaa ctgctgcggg
     5221 ataacaccac agggtctaat tcttccctca acacctcgct gccttcacct ggtgcctggc
     5281 cctacagtgc ctctgacaac tcctttacca acgttcatag cacttcagca aagttccctg
     5341 attacaaatc aacatggtcc ccagatccca taggacacaa ccccactcat ctctccaaca
     5401 agatgtggaa aaaccatatt tcctccagga acactacacc gctgccccgc ccacctcctg
     5461 gtctgaccaa ccccaaacca tcatctccct ggagcagcac agcaccccga tcagtcaggg
     5521 ggtgggggac acaggactca cggctcgcct cggcctctac ctggagtgat ggtggctcag
     5581 ttcgtcctag ttactggctg gttcttcaca atctcacccc acagattgat gggtcaacct
     5641 tgagaacgat ctgcatgcag catggcccac tgctgacatt ccatctgaat ctaacccagg
     5701 gcactgccct gatccgatac agcaccaaac aggaggcggc caaggcccaa actgcactgc
     5761 acatgtgtgt gttgggaaac actaccatcc ttgctgagtt tgccactgat gatgaagtca
     5821 gccgctttct ggcacaagct cagcccccta cacctgcagc aaccccaagt gcgccagctg
     5881 cggggtggca gtcgctggag accggccaga accagtcaga tcccgtggga cctgctctga
     5941 atctttttgg tgggtccact gggctcgggc agtggagcag cagcgctggt ggcagcagcg
     6001 gggccgatct tgctggcgct tcattgtggg ggcccccaaa ctattcttct agcttatggg
     6061 gagtcccaac ggtggaagat ccccatagga tgggcagccc tgctccttta ctacctggtg
     6121 accttctggg aggagggtcg gattcaatct gagcggccgc ttccctttag tgagggttaa
     6181 tgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     6241 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     6301 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     6361 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatccgat
     6421 aaggatcgat ccgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6481 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6901 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6961 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat
     7021 ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     7081 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7201 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7261 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7321 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7381 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7441 tgattcttct gacacaacag tctcgaactt aaggctagag ccaccatgat tgaacaagat
     7501 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     7561 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     7621 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     7681 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7741 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     7801 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7861 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7921 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7981 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     8041 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     8101 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     8161 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     8221 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     8281 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc
     8341 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     8401 gcgataagga tccgcgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8461 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8521 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     8581 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8641 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8821 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8881 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8941 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9001 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9061 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgaca gatct
//
