LOCUS       sequence-288752-       10687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delAGOhook.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delAGOhook_F
FEATURES             Location/Qualifiers
     source          1..10687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3530..3547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6391..6512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6402..6421)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6456..6475
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6698..7153
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6785..6804)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6995..7016
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7270..7627
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7270..7461
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7293..7313)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7478..7613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7678..8472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7732..7751)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8342..8361
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8536..8584
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8625..8644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8744..8766
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8804..8822)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8890..8994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8995..9063,9064..9855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9213..9232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10026..10614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10515..10534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccagt tgccaaggaa tgatcttgac ccaagagttc
     2581 tgtctaatac tggttgggga cagactcctg taaagcaaaa cactgcctgg gaatttgaag
     2641 aatcccctag gtctgaaagg aaaaatgaca atgggacaga ggcctggggt tgtgcagcta
     2701 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2761 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2821 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2881 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2941 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     3001 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     3061 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3121 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3181 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3241 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3301 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3361 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3421 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3481 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3541 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3601 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3661 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3721 ctaccctggc agagccgccg gtggcatttg gaagagctgg cgcacctgtt gctgcctcag
     3781 ccctgtgcaa accagcttca aaatctatgc aagaaggctg gggcagtggt ggggatgaaa
     3841 tgaacctcag taccagccag tgggaggatg aagaagggga cgtgtggaat aatgctgctt
     3901 cccaagaaag cacctcctcc tgcagctcct gggggaacgc ccccaaaaaa ggacttcaaa
     3961 agggcatgaa gacgtctggc aagcaggatg aggcctggat catgagccgg ctgatcaaac
     4021 aactcacaga catgggcttc ccgagagagc cagctgagga ggccttgaag agtaacaata
     4081 tgaatcttga tcaggccatg agcgctctgc tggaaaagaa ggtggacgtg gacaagcgtg
     4141 ggctgggagt gaccgaccat aatggaatgg ccgccaagcc cctcggctgc cgcccgccaa
     4201 tctccaaaga gtcttccgtg gaccgcccca cctttcttga caaggatggc ggcctcgtgg
     4261 aagagcccac gccttcaccg ttcttgcctt ccccaagcct gaagctcccc ctttcacaca
     4321 gtgcactccc cagtcaggcc ctgggtggga ttgcctccgg gctgggcatg caaaacttga
     4381 attcttctag acagataccg agtggcaatc tgggtatgtt tggcaatagt ggagcagcac
     4441 aagccaggac catgcagcag ccgccacagc caccagtgca gcctcttaac tcttcccagc
     4501 ccagtctccg tgctcaagtg cctcagtttc tatcccctca ggttcaagca cagcttttgc
     4561 agtttgcagc aaaaaacatt ggtctcaacc ctgcactatt aacctcgcca attaatcctc
     4621 aacatatgac gatgttgaac cagctctatc agctgcagct ggcataccaa cgtttacaaa
     4681 tccagcagca gatgttacag gcccagcgta atgtgtccgg atccatgaga caacaggagc
     4741 agcaagttgc gcgcacaatc actaatctgc agcagcagat ccagcagcac cagcgccagc
     4801 tggcccaggc cctgctcgtg aagcagccac caccgccacc gcccccgccg cacctgtctc
     4861 tgcacccctc tgcaggcaaa tcggccatgg acagcttccc ctcgcaccca cagactcccg
     4921 gcctacctga cctgcagacc aaagagcagc agtcttcacc caacaccttt gctccttacc
     4981 ctctcgctgg actgaaccca aacatgaatg tcaacagcat ggacatgacc ggtggcttgt
     5041 cggtgaagga cccatcccag tcccagtcac gcctccccca gtggacgcac cccaactcca
     5101 tggataactt gcccagtgcc gcttcccccc tggagcagaa ccctagcaag catggtgcta
     5161 tccctggagg tctaagcatt gggcctccag gtaagtcctc cattgatgac tcctatggcc
     5221 ggtacgattt aatccagaac agtgagtcac cagccagtcc tcccgtagct gttccccata
     5281 gctggtcacg tgccaaatct gacagtgata aaatctcaaa tggctctagc atcaactggc
     5341 ccccagaatt ccatccggga gttccatgga aaggactgca gaatattgac cctgagaatg
     5401 accctgacgt cactcctggc agtgtcccca ctgggcctac catcaacacc accatccagg
     5461 atgtcaaccg ctacctcctc aagagtggag gtaaactgtc agacatcaaa tcgacgtggt
     5521 cctctggccc tacctcccac acgcaagcct ctctgtctca tgaactatgg aaggtgccca
     5581 gaaacagtac tgcacccacg aggccacctc cagggttaac caatcccaag ccctcctcca
     5641 cctggggtgc cagccccctc ggctggacca gctcctactc ctcgggttct gcctggagca
     5701 ccgacacctc aggaagaacc agcagctggc tcgttcttcg aaacctcact ccccagattg
     5761 atggttctac actgcggaca ttgtgtttgc aacatgggcc tcttatcaca ttccacctga
     5821 atctgactca aggcaatgct gtggtccggt acagctccaa ggaggaggct gccaaggccc
     5881 agaagtctct gcacatgtgc gtcctgggaa acactaccat cctggccgag ttcgctggtg
     5941 aagaagaagt gaatcgcttc ttagcccaag gccaggcgct gccacccact tccagctggc
     6001 agtccagcag cgcgtccagc cagccgcggc tcagcgcagc gggcagctcc catggcctgg
     6061 tacgcagcga cgctggccac tggaacgccc cgtgcctggg tggcaagggg agcagtgagc
     6121 tgctgtgggg cggggtgccc cagtactcca gcagcctgtg gggcccgccc agcgccgacg
     6181 acagcagggt gataggcagc cccacgccgc taaccaccct gctgcctggg gacctgctca
     6241 gcggggagtc cctgtaggcg ctgccatcat cagcaccagg agagccgacc cctcccggga
     6301 cccctcccgg ctgggcggcc ccacagaccc gctggaaccc agcagcggcc gcttcccttt
     6361 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     6421 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6481 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     6541 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     6601 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
     6661 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     6721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6781 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6841 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6901 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6961 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7021 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7081 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7141 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7201 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     7261 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     7321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7381 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7441 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7501 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7561 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7621 ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg
     7681 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     7741 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     7801 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     7861 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     7921 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     7981 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     8041 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     8101 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     8161 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     8221 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     8281 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     8341 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     8401 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     8461 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     8521 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     8581 tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat
     8641 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8701 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8761 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     8821 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8881 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8941 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9001 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9061 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9121 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9181 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9241 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9301 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9361 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9421 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9481 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9541 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9601 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9661 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9721 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9781 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9841 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10141 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
    10681 cagatct
//
