LOCUS       sequence-288753-       10633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delMI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delMI_F
FEATURES             Location/Qualifiers
     source          1..10633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3410..3427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6337..6458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6348..6367)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6402..6421
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6644..7099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6731..6750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6941..6962
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7216..7573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7216..7407
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7239..7259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7424..7559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7624..8418
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7678..7697)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8288..8307
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8482..8530
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8571..8590)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8690..8712
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8750..8768)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8836..8940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8941..9009,9010..9801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9159..9178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9972..10560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10461..10480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccaga atgggacaga ggcctggggt tgtgcagcta
     2581 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2641 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2701 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2761 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2821 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     2881 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     2941 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3001 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3061 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3121 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3181 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3241 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3301 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3361 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3421 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3481 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3541 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3601 ctaccctggt ggataatggc acagcagcat gggggaagcc acccagcagt ggcagcgggt
     3661 ggggagatca ccctgccgag ccgccggtgg catttggaag agctggcgca cctgttgctg
     3721 cctcagccct gtgcaaacca gcttcaaaat ctatgcaaga aggctggggc agtggtgggg
     3781 atgaaatgaa cctcagtacc agccagtggg aggatgaaga aggggacgtg tggaataatg
     3841 ctgcttccca agaaagcacc tcctcctgca gctcctgggg gaacgccccc aaaaaaggac
     3901 ttcaaaaggg catgaagacg tctggcaagc aggatgaggc ctggatcatg agccggctga
     3961 tcaaacaact cacagacatg ggcttcccga gagagccagc tgaggaggcc ttgaagagta
     4021 acaatatgaa tcttgatcag gccatgagcg ctctgctgga aaagaaggtg gacgtggaca
     4081 agcgtgggct gggagtgacc gaccataatg gaatggccgc caagcccctc ggctgccgcc
     4141 cgccaatctc caaagagtct tccgtggacc gccccacctt tcttgacaag gatggcggcc
     4201 tcgtggaaga gcccacgcct tcaccgttct tgccttcccc aagcctgaag ctcccccttt
     4261 cacacagtgc actccccagt caggccctgg gtgggattgc ctccgggctg ggcatgcaaa
     4321 acttgaattc ttctagacag ataccgagtg gcaatctggg tatgtttggc aatagtggag
     4381 cagcacaagc caggaccatg cagcagccgc cacagccacc agtgcagcct cttaactctt
     4441 cccagcccag tctccgtgct caagtgcctc agtttctatc ccctcaggtt caagcacagc
     4501 ttttgcagtt tgcagcaaaa aacattggtc tcaaccctgc actattaacc tcgccaatta
     4561 atcctcaaca tatgacgatg ttgaaccagc tctatcagct gcagctggca taccaacgtt
     4621 tacaaatcca gcagcagatg ttacaggccc agcgtaatgt gtccggatcc atgagacaac
     4681 aggagcagca agttgcgcgc acaatcacta atctgcagca gcagatccag cagcaccagc
     4741 gccagctggc ccaggccctg ctcgtgaagc agccaccacc gccaccgccc ccgccgcacc
     4801 tgtctctgca cccctctgca ggcaaatcgg ccatggacag cttcccctcg cacccacaga
     4861 ctcccggcct acctgacctg cagaccaaag agcagcagtc ttcacccaac acctttgctc
     4921 cttaccctct cgctggactg aacccaaaca tgaatgtcaa cagcatggac atgaccggtg
     4981 gcttgtcggt gaaggaccca tcccagtccc agtcacgcct cccccagtgg acgcacccca
     5041 actccatgga taacttgccc agtgccgctt cccccctgga gcagaaccct agcaagcatg
     5101 gtgctatccc tggaggtcta agcattgggc ctccaggtaa gtcctccatt gatgactcct
     5161 atggccggta cgatttaatc cagaacagtg agtcaccagc cagtcctccc gtagctgttc
     5221 cccatagctg gtcacgtgcc aaatctgaca gtgataaaat ctcaaatggc tctagcatca
     5281 actggccccc agaattccat ccgggagttc catggaaagg actgcagaat attgaccctg
     5341 agaatgaccc tgacgtcact cctggcagtg tccccactgg gcctaccatc aacaccacca
     5401 tccaggatgt caaccgctac ctcctcaaga gtggaggtaa actgtcagac atcaaatcga
     5461 cgtggtcctc tggccctacc tcccacacgc aagcctctct gtctcatgaa ctatggaagg
     5521 tgcccagaaa cagtactgca cccacgaggc cacctccagg gttaaccaat cccaagccct
     5581 cctccacctg gggtgccagc cccctcggct ggaccagctc ctactcctcg ggttctgcct
     5641 ggagcaccga cacctcagga agaaccagca gctggctcgt tcttcgaaac ctcactcccc
     5701 agattgatgg ttctacactg cggacattgt gtttgcaaca tgggcctctt atcacattcc
     5761 acctgaatct gactcaaggc aatgctgtgg tccggtacag ctccaaggag gaggctgcca
     5821 aggcccagaa gtctctgcac atgtgcgtcc tgggaaacac taccatcctg gccgagttcg
     5881 ctggtgaaga agaagtgaat cgcttcttag cccaaggcca ggcgctgcca cccacttcca
     5941 gctggcagtc cagcagcgcg tccagccagc cgcggctcag cgcagcgggc agctcccatg
     6001 gcctggtacg cagcgacgct ggccactgga acgccccgtg cctgggtggc aaggggagca
     6061 gtgagctgct gtggggcggg gtgccccagt actccagcag cctgtggggc ccgcccagcg
     6121 ccgacgacag cagggtgata ggcagcccca cgccgctaac caccctgctg cctggggacc
     6181 tgctcagcgg ggagtccctg taggcgctgc catcatcagc accaggagag ccgacccctc
     6241 ccgggacccc tcccggctgg gcggccccac agacccgctg gaacccagca gcggccgctt
     6301 ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat gagtttggac
     6361 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6421 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6481 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     6541 aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag aggcccgcac
     6601 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     6661 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6721 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6781 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6841 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6901 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6961 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7021 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7081 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7141 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     7201 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     7261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7321 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7501 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7561 aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa ggctagagcc
     7621 accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     7801 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     7921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     8161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8461 acctgccatc acgatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     8521 tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag tacaatctgc
     8581 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     8641 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     8701 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     8761 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8821 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8881 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8941 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9001 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9061 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9121 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9181 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9241 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9301 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9361 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9421 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9481 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9541 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9601 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9661 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9721 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9781 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9841 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9901 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9961 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10021 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10081 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10141 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10201 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10261 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10321 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10381 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10441 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10501 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10561 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10621 gctcgacaga tct
//
