LOCUS       sequence-288754-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delMI-AGOhook.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delMI-AGOhook_F
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3410..3427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6271..6392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6282..6301)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6336..6355
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6578..7033
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6665..6684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6875..6896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7150..7507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7150..7341
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7173..7193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7358..7493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7558..8352
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7612..7631)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8222..8241
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8416..8464
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8505..8524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8624..8646
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8684..8702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8770..8874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8875..8943,8944..9735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9093..9112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9906..10494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10395..10414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccaga atgggacaga ggcctggggt tgtgcagcta
     2581 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2641 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2701 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2761 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2821 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     2881 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     2941 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3001 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3061 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3121 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3181 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3241 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3301 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3361 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3421 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3481 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3541 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3601 ctaccctggc agagccgccg gtggcatttg gaagagctgg cgcacctgtt gctgcctcag
     3661 ccctgtgcaa accagcttca aaatctatgc aagaaggctg gggcagtggt ggggatgaaa
     3721 tgaacctcag taccagccag tgggaggatg aagaagggga cgtgtggaat aatgctgctt
     3781 cccaagaaag cacctcctcc tgcagctcct gggggaacgc ccccaaaaaa ggacttcaaa
     3841 agggcatgaa gacgtctggc aagcaggatg aggcctggat catgagccgg ctgatcaaac
     3901 aactcacaga catgggcttc ccgagagagc cagctgagga ggccttgaag agtaacaata
     3961 tgaatcttga tcaggccatg agcgctctgc tggaaaagaa ggtggacgtg gacaagcgtg
     4021 ggctgggagt gaccgaccat aatggaatgg ccgccaagcc cctcggctgc cgcccgccaa
     4081 tctccaaaga gtcttccgtg gaccgcccca cctttcttga caaggatggc ggcctcgtgg
     4141 aagagcccac gccttcaccg ttcttgcctt ccccaagcct gaagctcccc ctttcacaca
     4201 gtgcactccc cagtcaggcc ctgggtggga ttgcctccgg gctgggcatg caaaacttga
     4261 attcttctag acagataccg agtggcaatc tgggtatgtt tggcaatagt ggagcagcac
     4321 aagccaggac catgcagcag ccgccacagc caccagtgca gcctcttaac tcttcccagc
     4381 ccagtctccg tgctcaagtg cctcagtttc tatcccctca ggttcaagca cagcttttgc
     4441 agtttgcagc aaaaaacatt ggtctcaacc ctgcactatt aacctcgcca attaatcctc
     4501 aacatatgac gatgttgaac cagctctatc agctgcagct ggcataccaa cgtttacaaa
     4561 tccagcagca gatgttacag gcccagcgta atgtgtccgg atccatgaga caacaggagc
     4621 agcaagttgc gcgcacaatc actaatctgc agcagcagat ccagcagcac cagcgccagc
     4681 tggcccaggc cctgctcgtg aagcagccac caccgccacc gcccccgccg cacctgtctc
     4741 tgcacccctc tgcaggcaaa tcggccatgg acagcttccc ctcgcaccca cagactcccg
     4801 gcctacctga cctgcagacc aaagagcagc agtcttcacc caacaccttt gctccttacc
     4861 ctctcgctgg actgaaccca aacatgaatg tcaacagcat ggacatgacc ggtggcttgt
     4921 cggtgaagga cccatcccag tcccagtcac gcctccccca gtggacgcac cccaactcca
     4981 tggataactt gcccagtgcc gcttcccccc tggagcagaa ccctagcaag catggtgcta
     5041 tccctggagg tctaagcatt gggcctccag gtaagtcctc cattgatgac tcctatggcc
     5101 ggtacgattt aatccagaac agtgagtcac cagccagtcc tcccgtagct gttccccata
     5161 gctggtcacg tgccaaatct gacagtgata aaatctcaaa tggctctagc atcaactggc
     5221 ccccagaatt ccatccggga gttccatgga aaggactgca gaatattgac cctgagaatg
     5281 accctgacgt cactcctggc agtgtcccca ctgggcctac catcaacacc accatccagg
     5341 atgtcaaccg ctacctcctc aagagtggag gtaaactgtc agacatcaaa tcgacgtggt
     5401 cctctggccc tacctcccac acgcaagcct ctctgtctca tgaactatgg aaggtgccca
     5461 gaaacagtac tgcacccacg aggccacctc cagggttaac caatcccaag ccctcctcca
     5521 cctggggtgc cagccccctc ggctggacca gctcctactc ctcgggttct gcctggagca
     5581 ccgacacctc aggaagaacc agcagctggc tcgttcttcg aaacctcact ccccagattg
     5641 atggttctac actgcggaca ttgtgtttgc aacatgggcc tcttatcaca ttccacctga
     5701 atctgactca aggcaatgct gtggtccggt acagctccaa ggaggaggct gccaaggccc
     5761 agaagtctct gcacatgtgc gtcctgggaa acactaccat cctggccgag ttcgctggtg
     5821 aagaagaagt gaatcgcttc ttagcccaag gccaggcgct gccacccact tccagctggc
     5881 agtccagcag cgcgtccagc cagccgcggc tcagcgcagc gggcagctcc catggcctgg
     5941 tacgcagcga cgctggccac tggaacgccc cgtgcctggg tggcaagggg agcagtgagc
     6001 tgctgtgggg cggggtgccc cagtactcca gcagcctgtg gggcccgccc agcgccgacg
     6061 acagcagggt gataggcagc cccacgccgc taaccaccct gctgcctggg gacctgctca
     6121 gcggggagtc cctgtaggcg ctgccatcat cagcaccagg agagccgacc cctcccggga
     6181 cccctcccgg ctgggcggcc ccacagaccc gctggaaccc agcagcggcc gcttcccttt
     6241 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     6301 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6361 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     6421 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     6481 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
     6541 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     6601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7021 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7081 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     7141 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     7201 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7261 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7321 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7381 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7441 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7501 ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg
     7561 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     7621 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     7681 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     7741 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     7801 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     7861 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     7921 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     7981 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     8041 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     8101 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     8161 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     8221 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     8281 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     8341 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     8401 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     8461 tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat
     8521 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8581 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8641 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     8701 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8761 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8821 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8881 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8941 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9001 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9061 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9121 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9181 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9241 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9301 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9361 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9421 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9481 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9541 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9601 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9661 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9721 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9781 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9841 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9901 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10021 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10081 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10141 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10201 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10261 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10321 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10381 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10441 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10501 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
    10561 cagatct
//
