LOCUS       sequence-288755-       10624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delMII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delMII_F
FEATURES             Location/Qualifiers
     source          1..10624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3401..3418
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6328..6449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6339..6358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6393..6412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6635..7090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6722..6741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6932..6953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7207..7564
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7207..7398
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7230..7250)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7415..7550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7615..8409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7669..7688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8279..8298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8473..8521
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8562..8581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8681..8703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8741..8759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8827..8931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8932..9000,9001..9792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9150..9169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9963..10551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10452..10471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccagt tgccaaggaa tgatcttgac ccaagagttc
     2581 tgtctaatac tggttgggga cagactcctg taaagcaaaa cactgcctgg gaatttgaag
     2641 aatcccctag gtctgaaagg aaaaatgaca atgggacaga ggcctggggt tgtgcagcta
     2701 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2761 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2821 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2881 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2941 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     3001 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     3061 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3121 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3181 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3241 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3301 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3361 gcagtaaccc ggtcatccag agcagtacca cgaccaatac caccaccacc accaccacta
     3421 ccacgagcaa caccacacac agggtcgaga cgccgccccc gcaccaggct ggtactcagc
     3481 tgaatcgatc accgttgctt ggtccaggta ggaaagtttc atcaggctgg ggagaaatgc
     3541 ctaatgttca ctcaaagact gaaaactctt ggggagaacc atcctcccct tctaccctgg
     3601 tggataatgg cacagcagca tgggggaagc cacccagcag tggcagcggg tggggagatc
     3661 accctgccga gccgccggtg gcatttggaa gagctggcgc acctgttgct gcctcagccc
     3721 tgtgcaaacc agcttcaaaa tctatgcaag aaggctgggg cagtggtggg gatgaaatga
     3781 acctcagtac cagccagtgg gaggatgaag aaggggacgt gtggaataat gctgcttccc
     3841 aagaaagcac ctcctcctgc agctcctggg ggaacgcccc caaaaaagga cttcaaaagg
     3901 gcatgaagac gtctggcaag caggatgagg cctggatcat gagccggctg atcaaacaac
     3961 tcacagacat gggcttcccg agagagccag ctgaggaggc cttgaagagt aacaatatga
     4021 atcttgatca ggccatgagc gctctgctgg aaaagaaggt ggacgtggac aagcgtgggc
     4081 tgggagtgac cgaccataat ggaatggccg ccaagcccct cggctgccgc ccgccaatct
     4141 ccaaagagtc ttccgtggac cgccccacct ttcttgacaa ggatggcggc ctcgtggaag
     4201 agcccacgcc ttcaccgttc ttgccttccc caagcctgaa gctccccctt tcacacagtg
     4261 cactccccag tcaggccctg ggtgggattg cctccgggct gggcatgcaa aacttgaatt
     4321 cttctagaca gataccgagt ggcaatctgg gtatgtttgg caatagtgga gcagcacaag
     4381 ccaggaccat gcagcagccg ccacagccac cagtgcagcc tcttaactct tcccagccca
     4441 gtctccgtgc tcaagtgcct cagtttctat cccctcaggt tcaagcacag cttttgcagt
     4501 ttgcagcaaa aaacattggt ctcaaccctg cactattaac ctcgccaatt aatcctcaac
     4561 atatgacgat gttgaaccag ctctatcagc tgcagctggc ataccaacgt ttacaaatcc
     4621 agcagcagat gttacaggcc cagcgtaatg tgtccggatc catgagacaa caggagcagc
     4681 aagttgcgcg cacaatcact aatctgcagc agcagatcca gcagcaccag cgccagctgg
     4741 cccaggccct gctcgtgaag cagccaccac cgccaccgcc cccgccgcac ctgtctctgc
     4801 acccctctgc aggcaaatcg gccatggaca gcttcccctc gcacccacag actcccggcc
     4861 tacctgacct gcagaccaaa gagcagcagt cttcacccaa cacctttgct ccttaccctc
     4921 tcgctggact gaacccaaac atgaatgtca acagcatgga catgaccggt ggcttgtcgg
     4981 tgaaggaccc atcccagtcc cagtcacgcc tcccccagtg gacgcacccc aactccatgg
     5041 ataacttgcc cagtgccgct tcccccctgg agcagaaccc tagcaagcat ggtgctatcc
     5101 ctggaggtct aagcattggg cctccaggta agtcctccat tgatgactcc tatggccggt
     5161 acgatttaat ccagaacagt gagtcaccag ccagtcctcc cgtagctgtt ccccatagct
     5221 ggtcacgtgc caaatctgac agtgataaaa tctcaaatgg ctctagcatc aactggcccc
     5281 cagaattcca tccgggagtt ccatggaaag gactgcagaa tattgaccct gagaatgacc
     5341 ctgacgtcac tcctggcagt gtccccactg ggcctaccat caacaccacc atccaggatg
     5401 tcaaccgcta cctcctcaag agtggaggta aactgtcaga catcaaatcg acgtggtcct
     5461 ctggccctac ctcccacacg caagcctctc tgtctcatga actatggaag gtgcccagaa
     5521 acagtactgc acccacgagg ccacctccag ggttaaccaa tcccaagccc tcctccacct
     5581 ggggtgccag ccccctcggc tggaccagct cctactcctc gggttctgcc tggagcaccg
     5641 acacctcagg aagaaccagc agctggctcg ttcttcgaaa cctcactccc cagattgatg
     5701 gttctacact gcggacattg tgtttgcaac atgggcctct tatcacattc cacctgaatc
     5761 tgactcaagg caatgctgtg gtccggtaca gctccaagga ggaggctgcc aaggcccaga
     5821 agtctctgca catgtgcgtc ctgggaaaca ctaccatcct ggccgagttc gctggtgaag
     5881 aagaagtgaa tcgcttctta gcccaaggcc aggcgctgcc acccacttcc agctggcagt
     5941 ccagcagcgc gtccagccag ccgcggctca gcgcagcggg cagctcccat ggcctggtac
     6001 gcagcgacgc tggccactgg aacgccccgt gcctgggtgg caaggggagc agtgagctgc
     6061 tgtggggcgg ggtgccccag tactccagca gcctgtgggg cccgcccagc gccgacgaca
     6121 gcagggtgat aggcagcccc acgccgctaa ccaccctgct gcctggggac ctgctcagcg
     6181 gggagtccct gtaggcgctg ccatcatcag caccaggaga gccgacccct cccgggaccc
     6241 ctcccggctg ggcggcccca cagacccgct ggaacccagc agcggccgct tccctttagt
     6301 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     6361 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6421 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6481 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6541 aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc
     6601 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     6661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6961 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7081 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7141 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     7201 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7261 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7381 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7441 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7501 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7561 caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt
     7621 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7681 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7741 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7801 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7861 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7921 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7981 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8041 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8101 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8161 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8221 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8281 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8341 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8401 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8461 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     8521 gaatcgatag cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc
     8581 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8641 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8701 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8761 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8821 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8881 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8941 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9001 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9061 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9121 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9181 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9241 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9301 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9361 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9421 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9481 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9541 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9601 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9661 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9721 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9781 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9841 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9901 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9961 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10021 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10081 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10141 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10201 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10261 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10321 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10381 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10441 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10501 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10561 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag
    10621 atct
//
