LOCUS       sequence-288756-       10504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delMI-MII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII_F
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3281..3298
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6208..6329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6219..6238)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6273..6292
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6515..6970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6602..6621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6812..6833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7087..7444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7087..7278
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7110..7130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7295..7430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7495..8289
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7549..7568)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8159..8178
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8353..8401
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8442..8461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8561..8583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8621..8639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8707..8811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8812..8880,8881..9672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9030..9049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9843..10431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10332..10351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccaga atgggacaga ggcctggggt tgtgcagcta
     2581 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2641 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2701 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2761 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2821 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     2881 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     2941 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3001 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3061 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3121 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3181 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3241 gcagtaaccc ggtcatccag agcagtacca cgaccaatac caccaccacc accaccacta
     3301 ccacgagcaa caccacacac agggtcgaga cgccgccccc gcaccaggct ggtactcagc
     3361 tgaatcgatc accgttgctt ggtccaggta ggaaagtttc atcaggctgg ggagaaatgc
     3421 ctaatgttca ctcaaagact gaaaactctt ggggagaacc atcctcccct tctaccctgg
     3481 tggataatgg cacagcagca tgggggaagc cacccagcag tggcagcggg tggggagatc
     3541 accctgccga gccgccggtg gcatttggaa gagctggcgc acctgttgct gcctcagccc
     3601 tgtgcaaacc agcttcaaaa tctatgcaag aaggctgggg cagtggtggg gatgaaatga
     3661 acctcagtac cagccagtgg gaggatgaag aaggggacgt gtggaataat gctgcttccc
     3721 aagaaagcac ctcctcctgc agctcctggg ggaacgcccc caaaaaagga cttcaaaagg
     3781 gcatgaagac gtctggcaag caggatgagg cctggatcat gagccggctg atcaaacaac
     3841 tcacagacat gggcttcccg agagagccag ctgaggaggc cttgaagagt aacaatatga
     3901 atcttgatca ggccatgagc gctctgctgg aaaagaaggt ggacgtggac aagcgtgggc
     3961 tgggagtgac cgaccataat ggaatggccg ccaagcccct cggctgccgc ccgccaatct
     4021 ccaaagagtc ttccgtggac cgccccacct ttcttgacaa ggatggcggc ctcgtggaag
     4081 agcccacgcc ttcaccgttc ttgccttccc caagcctgaa gctccccctt tcacacagtg
     4141 cactccccag tcaggccctg ggtgggattg cctccgggct gggcatgcaa aacttgaatt
     4201 cttctagaca gataccgagt ggcaatctgg gtatgtttgg caatagtgga gcagcacaag
     4261 ccaggaccat gcagcagccg ccacagccac cagtgcagcc tcttaactct tcccagccca
     4321 gtctccgtgc tcaagtgcct cagtttctat cccctcaggt tcaagcacag cttttgcagt
     4381 ttgcagcaaa aaacattggt ctcaaccctg cactattaac ctcgccaatt aatcctcaac
     4441 atatgacgat gttgaaccag ctctatcagc tgcagctggc ataccaacgt ttacaaatcc
     4501 agcagcagat gttacaggcc cagcgtaatg tgtccggatc catgagacaa caggagcagc
     4561 aagttgcgcg cacaatcact aatctgcagc agcagatcca gcagcaccag cgccagctgg
     4621 cccaggccct gctcgtgaag cagccaccac cgccaccgcc cccgccgcac ctgtctctgc
     4681 acccctctgc aggcaaatcg gccatggaca gcttcccctc gcacccacag actcccggcc
     4741 tacctgacct gcagaccaaa gagcagcagt cttcacccaa cacctttgct ccttaccctc
     4801 tcgctggact gaacccaaac atgaatgtca acagcatgga catgaccggt ggcttgtcgg
     4861 tgaaggaccc atcccagtcc cagtcacgcc tcccccagtg gacgcacccc aactccatgg
     4921 ataacttgcc cagtgccgct tcccccctgg agcagaaccc tagcaagcat ggtgctatcc
     4981 ctggaggtct aagcattggg cctccaggta agtcctccat tgatgactcc tatggccggt
     5041 acgatttaat ccagaacagt gagtcaccag ccagtcctcc cgtagctgtt ccccatagct
     5101 ggtcacgtgc caaatctgac agtgataaaa tctcaaatgg ctctagcatc aactggcccc
     5161 cagaattcca tccgggagtt ccatggaaag gactgcagaa tattgaccct gagaatgacc
     5221 ctgacgtcac tcctggcagt gtccccactg ggcctaccat caacaccacc atccaggatg
     5281 tcaaccgcta cctcctcaag agtggaggta aactgtcaga catcaaatcg acgtggtcct
     5341 ctggccctac ctcccacacg caagcctctc tgtctcatga actatggaag gtgcccagaa
     5401 acagtactgc acccacgagg ccacctccag ggttaaccaa tcccaagccc tcctccacct
     5461 ggggtgccag ccccctcggc tggaccagct cctactcctc gggttctgcc tggagcaccg
     5521 acacctcagg aagaaccagc agctggctcg ttcttcgaaa cctcactccc cagattgatg
     5581 gttctacact gcggacattg tgtttgcaac atgggcctct tatcacattc cacctgaatc
     5641 tgactcaagg caatgctgtg gtccggtaca gctccaagga ggaggctgcc aaggcccaga
     5701 agtctctgca catgtgcgtc ctgggaaaca ctaccatcct ggccgagttc gctggtgaag
     5761 aagaagtgaa tcgcttctta gcccaaggcc aggcgctgcc acccacttcc agctggcagt
     5821 ccagcagcgc gtccagccag ccgcggctca gcgcagcggg cagctcccat ggcctggtac
     5881 gcagcgacgc tggccactgg aacgccccgt gcctgggtgg caaggggagc agtgagctgc
     5941 tgtggggcgg ggtgccccag tactccagca gcctgtgggg cccgcccagc gccgacgaca
     6001 gcagggtgat aggcagcccc acgccgctaa ccaccctgct gcctggggac ctgctcagcg
     6061 gggagtccct gtaggcgctg ccatcatcag caccaggaga gccgacccct cccgggaccc
     6121 ctcccggctg ggcggcccca cagacccgct ggaacccagc agcggccgct tccctttagt
     6181 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     6241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6301 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6361 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6421 aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc
     6481 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     6541 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6601 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6661 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6721 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6781 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6841 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6901 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6961 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7021 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     7081 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7201 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7261 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7381 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7441 caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt
     7501 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7561 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7621 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7681 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7741 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7801 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7861 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7921 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7981 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8041 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8101 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8161 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8221 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8281 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8341 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     8401 gaatcgatag cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc
     8461 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8521 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8581 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8641 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8701 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8761 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8821 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8881 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8941 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9001 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9061 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9121 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9181 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9241 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9301 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9361 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9421 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9481 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9541 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9601 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9661 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9721 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9961 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10201 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10261 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10321 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10381 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10441 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag
    10501 atct
//
