LOCUS       sequence-288757-       10438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delMI-MII-AGOhook.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII-AGOhook_F
FEATURES             Location/Qualifiers
     source          1..10438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3281..3298
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6142..6263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6153..6172)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6207..6226
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6449..6904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6536..6555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6746..6767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7021..7378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7021..7212
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7044..7064)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7229..7364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7429..8223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7483..7502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8093..8112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8287..8335
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8376..8395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8495..8517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8555..8573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8641..8745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8746..8814,8815..9606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8964..8983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9777..10365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10266..10285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccaga atgggacaga ggcctggggt tgtgcagcta
     2581 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2641 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2701 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2761 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2821 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     2881 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     2941 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3001 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3061 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3121 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3181 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3241 gcagtaaccc ggtcatccag agcagtacca cgaccaatac caccaccacc accaccacta
     3301 ccacgagcaa caccacacac agggtcgaga cgccgccccc gcaccaggct ggtactcagc
     3361 tgaatcgatc accgttgctt ggtccaggta ggaaagtttc atcaggctgg ggagaaatgc
     3421 ctaatgttca ctcaaagact gaaaactctt ggggagaacc atcctcccct tctaccctgg
     3481 cagagccgcc ggtggcattt ggaagagctg gcgcacctgt tgctgcctca gccctgtgca
     3541 aaccagcttc aaaatctatg caagaaggct ggggcagtgg tggggatgaa atgaacctca
     3601 gtaccagcca gtgggaggat gaagaagggg acgtgtggaa taatgctgct tcccaagaaa
     3661 gcacctcctc ctgcagctcc tgggggaacg cccccaaaaa aggacttcaa aagggcatga
     3721 agacgtctgg caagcaggat gaggcctgga tcatgagccg gctgatcaaa caactcacag
     3781 acatgggctt cccgagagag ccagctgagg aggccttgaa gagtaacaat atgaatcttg
     3841 atcaggccat gagcgctctg ctggaaaaga aggtggacgt ggacaagcgt gggctgggag
     3901 tgaccgacca taatggaatg gccgccaagc ccctcggctg ccgcccgcca atctccaaag
     3961 agtcttccgt ggaccgcccc acctttcttg acaaggatgg cggcctcgtg gaagagccca
     4021 cgccttcacc gttcttgcct tccccaagcc tgaagctccc cctttcacac agtgcactcc
     4081 ccagtcaggc cctgggtggg attgcctccg ggctgggcat gcaaaacttg aattcttcta
     4141 gacagatacc gagtggcaat ctgggtatgt ttggcaatag tggagcagca caagccagga
     4201 ccatgcagca gccgccacag ccaccagtgc agcctcttaa ctcttcccag cccagtctcc
     4261 gtgctcaagt gcctcagttt ctatcccctc aggttcaagc acagcttttg cagtttgcag
     4321 caaaaaacat tggtctcaac cctgcactat taacctcgcc aattaatcct caacatatga
     4381 cgatgttgaa ccagctctat cagctgcagc tggcatacca acgtttacaa atccagcagc
     4441 agatgttaca ggcccagcgt aatgtgtccg gatccatgag acaacaggag cagcaagttg
     4501 cgcgcacaat cactaatctg cagcagcaga tccagcagca ccagcgccag ctggcccagg
     4561 ccctgctcgt gaagcagcca ccaccgccac cgcccccgcc gcacctgtct ctgcacccct
     4621 ctgcaggcaa atcggccatg gacagcttcc cctcgcaccc acagactccc ggcctacctg
     4681 acctgcagac caaagagcag cagtcttcac ccaacacctt tgctccttac cctctcgctg
     4741 gactgaaccc aaacatgaat gtcaacagca tggacatgac cggtggcttg tcggtgaagg
     4801 acccatccca gtcccagtca cgcctccccc agtggacgca ccccaactcc atggataact
     4861 tgcccagtgc cgcttccccc ctggagcaga accctagcaa gcatggtgct atccctggag
     4921 gtctaagcat tgggcctcca ggtaagtcct ccattgatga ctcctatggc cggtacgatt
     4981 taatccagaa cagtgagtca ccagccagtc ctcccgtagc tgttccccat agctggtcac
     5041 gtgccaaatc tgacagtgat aaaatctcaa atggctctag catcaactgg cccccagaat
     5101 tccatccggg agttccatgg aaaggactgc agaatattga ccctgagaat gaccctgacg
     5161 tcactcctgg cagtgtcccc actgggccta ccatcaacac caccatccag gatgtcaacc
     5221 gctacctcct caagagtgga ggtaaactgt cagacatcaa atcgacgtgg tcctctggcc
     5281 ctacctccca cacgcaagcc tctctgtctc atgaactatg gaaggtgccc agaaacagta
     5341 ctgcacccac gaggccacct ccagggttaa ccaatcccaa gccctcctcc acctggggtg
     5401 ccagccccct cggctggacc agctcctact cctcgggttc tgcctggagc accgacacct
     5461 caggaagaac cagcagctgg ctcgttcttc gaaacctcac tccccagatt gatggttcta
     5521 cactgcggac attgtgtttg caacatgggc ctcttatcac attccacctg aatctgactc
     5581 aaggcaatgc tgtggtccgg tacagctcca aggaggaggc tgccaaggcc cagaagtctc
     5641 tgcacatgtg cgtcctggga aacactacca tcctggccga gttcgctggt gaagaagaag
     5701 tgaatcgctt cttagcccaa ggccaggcgc tgccacccac ttccagctgg cagtccagca
     5761 gcgcgtccag ccagccgcgg ctcagcgcag cgggcagctc ccatggcctg gtacgcagcg
     5821 acgctggcca ctggaacgcc ccgtgcctgg gtggcaaggg gagcagtgag ctgctgtggg
     5881 gcggggtgcc ccagtactcc agcagcctgt ggggcccgcc cagcgccgac gacagcaggg
     5941 tgataggcag ccccacgccg ctaaccaccc tgctgcctgg ggacctgctc agcggggagt
     6001 ccctgtaggc gctgccatca tcagcaccag gagagccgac ccctcccggg acccctcccg
     6061 gctgggcggc cccacagacc cgctggaacc cagcagcggc cgcttccctt tagtgagggt
     6121 taatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     6181 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6241 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     6301 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc
     6361 gataaggatc gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6421 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     6481 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6541 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6601 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6661 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6721 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6781 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6841 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6901 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
     6961 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
     7021 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7081 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7141 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7201 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7261 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7381 gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa
     7441 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7501 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7561 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     7621 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7681 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     7741 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     7801 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     7861 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7921 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     7981 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8041 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8101 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8161 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8221 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     8281 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     8341 atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8401 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8521 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8581 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8641 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8701 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8761 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8821 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8881 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8941 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     9001 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9061 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9121 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9181 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9241 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9301 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9361 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9421 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9481 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9541 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9601 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9661 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct
//
