LOCUS       sequence-288793-        8408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP2-PC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8408)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmDCP2-PC-V5His6_G
FEATURES             Location/Qualifiers
     source          1..8408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5693..5734
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5744..5761
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5784..5801)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5834..5968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5858..5877)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5912..5931
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6590..7178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6670..6689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7349..8140,8141..8209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7972..7991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8210..8314)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8382..8400
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgcatga ttgccattaa
     3361 agtcaacaat ctagcgttgc tcgcgtcgct gactttacaa aaagtgctaa atatcaacaa
     3421 taacaataac cagcacagca agaacaacaa ccacagcgac aatagcaata gcaaacaaca
     3481 cgagcaacaa aaacagcagc agcaaccaca acgacgaaac ctcgacatta aaagcgagta
     3541 tgatgtacta aaccaattgc taaaaactgg agctgttttc accgccacgc ccacagcgcc
     3601 aattgccatc gaaaggagga agcagacgca gcagcagcaa cgtccatatg cacaacaggc
     3661 acatcaacag cagcagcaga agcaacgcct aaactcatcc atatcatcga cccactcatc
     3721 gacatcgaca aactcctcct acggcagctc atcatcggag gatgctgcag gcacatctgc
     3781 gccaacggga gcaggtgaag ttgtcacatc agcagcatta acaccaagag caagcaccac
     3841 gaaggcgtcc agcaataaat tgccagagaa atcgaaaatt ccgtccgata tacttgatga
     3901 tctggccagt cgatttataa tcaatgttcc ggacatggag ctaaacaatc taatacgtat
     3961 gtgtttccaa atcgaactgg cccattggtt ctatttggat ttcttttgtg cccccgaatc
     4021 tggcgaggat ggcgagaccc cgaagtgcgt gcaacgaaag ctgccatcgg tgggcatcaa
     4081 gcagtttgcc atgcagttgt tccaacacat tcccttcctg aacaaacatt ttggcaccgt
     4141 ggatcaaatc ctggacgagt ggaagaacta caagctatcg gtgcccactt atggcgctat
     4201 tctggtgtcc gaggatcaca atcactgctt gctagtgcag tcctacttcg cgcgcaactc
     4261 ctggggattt cccaagggca agatcaatga gaacgaagac ccagcccatt gtgcaacgag
     4321 agaggcaagt cgggaatata ttggaattct aaaaaatatt attgtttatg aggagaccgg
     4381 gttcgatatc acggacctaa tcgatgccaa cgactacatc gaggcgttca tcaactacca
     4441 gtacacgcgg ctgtacgtgg tgcgaaacat accgatggac acgcagttcg cgccccgcac
     4501 tcgcaacgag atcaagtgct gcgactggtt ccgtatcgat gcgctgccgg tgaacaagaa
     4561 cgatgccata tcaaaggcca agttgggcaa gacctccaac tccttcttca tgattatgcc
     4621 gtttgtgaag cgcctgaaga agtgggtgaa cgatcggaag gctggaatcg agccgcgtcg
     4681 ccgcaaattc tctggtcagc aatcgccaaa agcggcctcg cccaccaata tgaacggaaa
     4741 ttgcaagaag gacgtcacag gagtgcgaaa tgaatcgcaa cgacagaggc acaagtccat
     4801 gggcgacttg gatggcgtca aattgaataa tctcaatggc aaggggaccg ttggaacggg
     4861 agcgggatcg gcagcttccg cctccgccac cgctgccatc aactcgatga acatatgcaa
     4921 tagcaatggg aaacgcaata agcaaaatgc ggctgcgggc agcaatcagg caacgccaac
     4981 tacaatagca acgccaatga cgtcaaatgg cgccgtcgta ggaggtactg tatcctcgaa
     5041 gcgtcaactg ttccatagcc aaagtcagaa cgatgcgcag caatcggcaa gcaatgagaa
     5101 gcagattgtc agctccttcg atttaatacg caaggagaag cagcaacaac ttaaggcagc
     5161 taaggcgcaa aagcagcagc aacaacagca gcaacgatcg cgcaccaagt cgcagagtga
     5221 taagcagtac aatccacagc agccagtcaa aatcctggga caacagcagc ccacaacggg
     5281 catggatttg atagccatgc tatcggcagc ggcggcagct caaaagacac caaacaacga
     5341 gcagcaaaca caagagaagc agcagcagca acaacgacca cgacctgctt cagtgtcgct
     5401 taaccttgga gcaggcagtg ccacaggagc aacaacatca gctccggatg cccagtacat
     5461 gcacaggctg cagcgcatgg catcgggcac aaatttgagg attctgaagc gggcacagtc
     5521 gcagcagccg cagcagcacc atcagcaaca gttgcaacta gaacagcaac aacagcagca
     5581 gcagcagctg ggaatcgact ttacgccaaa tttgaattcg tggacgaatt tctcctttac
     5641 caagaacttc atagcaaatg tgttttgcag tctagagggc ccgcggttcg aaggtaagcc
     5701 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     5761 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     5821 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     5881 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5941 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     6001 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     6061 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     6121 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     6181 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     6241 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     6301 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     6361 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     6421 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     6481 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     6541 ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6781 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7021 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7261 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7321 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7381 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7441 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7501 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7561 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7621 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     7681 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7741 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7801 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7861 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7921 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     7981 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8041 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8101 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8161 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8221 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8281 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8341 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8401 cctttcgt
//
