LOCUS       sequence-288801-        9207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1_574-1613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9207)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmXRN1_574-1613_G
FEATURES             Location/Qualifiers
     source          1..9207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6492..6533
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6543..6560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6583..6600)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6633..6767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6657..6676)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6711..6730
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7389..7977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7469..7488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8148..8939,8940..9008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8771..8790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9009..9113)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9181..9199
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgcatgc gacacggtcc
     3361 catgtatgtg tacaaataca gtacggtggc gcagggtccc atgccagcgt atccaccgct
     3421 gagggctctt cccgtgctct actgcacgga ggtggccaag tggtcgcacg aaatcgcagt
     3481 caatctgccg tacagcgtgt gcatcgagct accaaatgcg gcgagaaccg ttttcttccc
     3541 gggatttccc accatgcagc atctgccctt cgatttcgag ctgcgcaacg accgcgtcaa
     3601 ggtgttcgaa caggtcagtc gcaaccagaa cattgtgcta aaaccacgca agcgccagct
     3661 ggaggacaca ctgacggcgg tggcctccca gtacctcggg aaggtgatcc acgtcggctg
     3721 gccgcatctc gtcaaggcga tagtggtgcg cgtggccacg cgagatcagc gcgtcgacag
     3781 cgagggaatc acgctaaacg attcgcggcg tttcgactcg gaatgcaagg cactgcagga
     3841 acactttatc aatcgtatgg gaatacagtt tgccaactat gatgtgctcg tctatgtgcg
     3901 cacctttgcc ggcaattcga cggagttccg cgacaagggg gcattgatgg tgcgcgactc
     3961 gtggagcagc tccgtcaccg gctatccagc ccagggcgtg gtggccgatc tgactgtgtg
     4021 ggaaagaatg cgcaagaatt tcctcaacgt ggagcactac ttccccgttg gcagcacgat
     4081 cttcttgatc acggatccgt attacggcag cgagggaact gtgcaggacc cacggctggc
     4141 ctacaccaac ggccggattc aagtcagcat catggtgcga ccggaaccga aagtaaatgc
     4201 ggcacgtcag ctgcaggaag agcgggaccg ggactatctg agcaccttcc aggtctgcaa
     4261 cttgctccga atcagtggcc gcaccctggg acgcctaagt ggcaccgtgt gggtggtgct
     4321 cggtccgcgg cgtcagaaga tggaaaacgt gaccaagcac aacattggcc tgcagctcaa
     4381 gtatccgcgg cagaacgagg agcgtgccgg atactgcttc cgcacgaaca accagtggta
     4441 ctactccagc ctggccgtgg atctgatgcg taactattgc cagcgttacc cagacgttat
     4501 cgacttcttt ggcgactcca acgatcgcgc cgagttcgta ttcgaacagg acgtgttccc
     4561 caatgcggtt ggccatcgcc gcgtcgagga actggccaac tgggtgcgcc agcagccgca
     4621 catgaaagtg gagcgcatct cttgcggctc caagacggtc tgccgcgaga ccatcgaact
     4681 gctgatagcc gccgtcgacg acctgcgctc actgcccgtc aagcacgtta aactgcaggt
     4741 gaagccgcac ttgctcatca agcccaatgt cacactgcca gatgtctacc gatcaaagcg
     4801 cccggtgcgt ctcttcgatc gcgtcgtaat cgtacgcacc atttacatgg tgcccgtggg
     4861 cactaagggc accgtgattg gcattcatcc cgttacggac cccaacccgg tccgcctgga
     4921 gtgcgtccat gctgtcgaca cgttctgcaa ggtgctgttc gacagccccg ttccgaactg
     4981 caacaacatc catggcatcg ccgaagaccg agtttacaag gtgcccgaaa ttgccctggt
     5041 gatcatcaag acggatgagg aaggaaagaa gcagaacgac tgcgaactgc cggtgaggga
     5101 tcctcagccg aaccaggcac aggacgagcc agtccgcgcg accagcagtc gctatgtgac
     5161 cgccgcaggg tccacctcgg tcccgatcac gatgaagacc caaattagcg acgaattcgt
     5221 caagacgcgt agcgatccga tagcccgtac ggattcgtac aagccatcgt cggagccaaa
     5281 accggtgccg gtgccggagc agattaccaa ctggcgggaa cgcgtcagta cgccaacaaa
     5341 caaaccgcag cctgcgccaa ataattggcg cataaatcgg tcttcatcac gtcaacaggg
     5401 cggtagtatt tttgttgctc cgcccacaaa aactcctgat gcagctgcaa gcactgcttc
     5461 cactgcattc accgcagcct cgtccgcaac gctgactcct ctggatcaga ccttggcgct
     5521 catgtcggtt ttgggcgtgg gtgaggacca gagctcgccg ccactccagg aggcggtgca
     5581 gcagcaacgt ccgccactac ttcaacagca aagagctccg tttcccggac aaatgcccaa
     5641 tctacccaag ccaccgctct tctggcagca agaggcgcag aagcaggagg cgctgcagca
     5701 ggaggcgcag cagcaggagg cgcagaagaa gcagcagcag gcgcatgcgc aaatggagcc
     5761 tgaacgtatc aatagccagc atttctacag aagcggccag acaggagcgg cgttgaatca
     5821 gccgccattg ggtgctccat caaaaaggca gtggcatgag tgggtgcacc cgcgcatgca
     5881 gcacgccaac gcattccacg ctggcgtcaa caatggatat cagatgcgtc ccaaaaagaa
     5941 tatagctgcg cagagcacct ttaacaacaa cgtgcacatg cacctccagc aaccatatta
     6001 cccgaatcag cagcagcagc agcagcagca gcagccactc cagctgacgg agatcaacaa
     6061 tgcgccaccg cgatactcaa cgatccaaga ttttgtgccc atacaggcgt accgtcccaa
     6121 gaaattaaat cgcgtccaac cagccggtcg tcaggacgtc gatgccacca agaatcctag
     6181 tcgttcgcct gtgttgcagc aaccgacgaa cgagactata gacacaaagg ccagttctag
     6241 tcttccggta caatcagcag gcgaacaggt gattggactg atgcaaacgc ttgaaattaa
     6301 gccggctgcg tcgcagtccg aatctgatgg ggtctcaaca ggcagtgcga acgcgcccac
     6361 agcaacgaca tcatcccagg cggtcaaccg aaggaagcat cgagtgcctc gcatcggagc
     6421 caagttcgac ttggaataca tactgcccga ctccccgcat ccaacttaat ctagagggcc
     6481 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     6541 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     6601 aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg
     6661 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6721 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6781 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     6841 acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc
     6901 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     6961 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     7021 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     7081 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
     7141 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
     7201 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     7261 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     7321 ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7381 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7441 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7501 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7561 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     7621 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7681 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7741 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7801 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7861 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7921 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7981 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8041 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8101 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8161 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8221 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8281 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8341 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8401 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8461 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8521 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8581 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8641 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8701 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8761 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8821 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8881 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8941 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9001 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9061 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9121 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9181 aataggcgta tcacgaggcc ctttcgt
//
