LOCUS       sequence-288805-        7811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP2-PC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7811)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmDCP2-PC-V5His6_G
FEATURES             Location/Qualifiers
     source          1..7811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5096..5137
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5147..5164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5187..5204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5237..5371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5261..5280)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5315..5334
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5993..6581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6073..6092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6752..7543,7544..7612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7375..7394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7613..7717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7785..7803
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgattgccat
     2761 taaagtcaac aatctagcgt tgctcgcgtc gctgacttta caaaaagtgc taaatatcaa
     2821 caataacaat aaccagcaca gcaagaacaa caaccacagc gacaatagca atagcaaaca
     2881 acacgagcaa caaaaacagc agcagcaacc acaacgacga aacctcgaca ttaaaagcga
     2941 gtatgatgta ctaaaccaat tgctaaaaac tggagctgtt ttcaccgcca cgcccacagc
     3001 gccaattgcc atcgaaagga ggaagcagac gcagcagcag caacgtccat atgcacaaca
     3061 ggcacatcaa cagcagcagc agaagcaacg cctaaactca tccatatcat cgacccactc
     3121 atcgacatcg acaaactcct cctacggcag ctcatcatcg gaggatgctg caggcacatc
     3181 tgcgccaacg ggagcaggtg aagttgtcac atcagcagca ttaacaccaa gagcaagcac
     3241 cacgaaggcg tccagcaata aattgccaga gaaatcgaaa attccgtccg atatacttga
     3301 tgatctggcc agtcgattta taatcaatgt tccggacatg gagctaaaca atctaatacg
     3361 tatgtgtttc caaatcgaac tggcccattg gttctatttg gatttctttt gtgcccccga
     3421 atctggcgag gatggcgaga ccccgaagtg cgtgcaacga aagctgccat cggtgggcat
     3481 caagcagttt gccatgcagt tgttccaaca cattcccttc ctgaacaaac attttggcac
     3541 cgtggatcaa atcctggacg agtggaagaa ctacaagcta tcggtgccca cttatggcgc
     3601 tattctggtg tccgaggatc acaatcactg cttgctagtg cagtcctact tcgcgcgcaa
     3661 ctcctgggga tttcccaagg gcaagatcaa tgagaacgaa gacccagccc attgtgcaac
     3721 gagagaggca agtcgggaat atattggaat tctaaaaaat attattgttt atgaggagac
     3781 cgggttcgat atcacggacc taatcgatgc caacgactac atcgaggcgt tcatcaacta
     3841 ccagtacacg cggctgtacg tggtgcgaaa cataccgatg gacacgcagt tcgcgccccg
     3901 cactcgcaac gagatcaagt gctgcgactg gttccgtatc gatgcgctgc cggtgaacaa
     3961 gaacgatgcc atatcaaagg ccaagttggg caagacctcc aactccttct tcatgattat
     4021 gccgtttgtg aagcgcctga agaagtgggt gaacgatcgg aaggctggaa tcgagccgcg
     4081 tcgccgcaaa ttctctggtc agcaatcgcc aaaagcggcc tcgcccacca atatgaacgg
     4141 aaattgcaag aaggacgtca caggagtgcg aaatgaatcg caacgacaga ggcacaagtc
     4201 catgggcgac ttggatggcg tcaaattgaa taatctcaat ggcaagggga ccgttggaac
     4261 gggagcggga tcggcagctt ccgcctccgc caccgctgcc atcaactcga tgaacatatg
     4321 caatagcaat gggaaacgca ataagcaaaa tgcggctgcg ggcagcaatc aggcaacgcc
     4381 aactacaata gcaacgccaa tgacgtcaaa tggcgccgtc gtaggaggta ctgtatcctc
     4441 gaagcgtcaa ctgttccata gccaaagtca gaacgatgcg cagcaatcgg caagcaatga
     4501 gaagcagatt gtcagctcct tcgatttaat acgcaaggag aagcagcaac aacttaaggc
     4561 agctaaggcg caaaagcagc agcaacaaca gcagcaacga tcgcgcacca agtcgcagag
     4621 tgataagcag tacaatccac agcagccagt caaaatcctg ggacaacagc agcccacaac
     4681 gggcatggat ttgatagcca tgctatcggc agcggcggca gctcaaaaga caccaaacaa
     4741 cgagcagcaa acacaagaga agcagcagca gcaacaacga ccacgacctg cttcagtgtc
     4801 gcttaacctt ggagcaggca gtgccacagg agcaacaaca tcagctccgg atgcccagta
     4861 catgcacagg ctgcagcgca tggcatcggg cacaaatttg aggattctga agcgggcaca
     4921 gtcgcagcag ccgcagcagc accatcagca acagttgcaa ctagaacagc aacaacagca
     4981 gcagcagcag ctgggaatcg actttacgcc aaatttgaat tcgtggacga atttctcctt
     5041 taccaagaac ttcatagcaa atgtgttttg cagtctagag ggcccgcggt tcgaaggtaa
     5101 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     5161 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg
     5221 atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     5281 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     5341 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     5401 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     5461 tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     5521 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     5581 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     5641 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     5701 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     5761 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     5821 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     5881 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     5941 ttaccagcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6181 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6421 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     7081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     7381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7741 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     7801 ggccctttcg t
//
