LOCUS       sequence-288810-        8617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmImp8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8617)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmImp8_G
FEATURES             Location/Qualifiers
     source          1..8617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5902..5943
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5953..5970
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5993..6010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6043..6177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6067..6086)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6121..6140
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6799..7387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6879..6898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7558..8349,8350..8418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8181..8200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8419..8523)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8591..8609
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgg aggcacaaaa actcacggaa ctgttgcgcg caactattga
     2761 tccgaatccg gaacagcgca aggcggccga ggatcaactg gcccagatac acaaaatcat
     2821 cggatttgtg cccaccatac tgcagatcgt tatgcagacg acggtggaac agccggtgcg
     2881 ccaggccggt gctgtctacc tcaagaatct gatcaacagc agctggtcgg accacgaggc
     2941 caagcctgga gagccaatac ccttctctat acacgaacag gatcgggcta tgatccgtgg
     3001 cgctattgta gatgctattg tgcacgcccc cgagttgatt agggtccagt tgtctgtctg
     3061 cgtcaatcac atcattaaaa gcgatttccc tggacgctgg ccacaggtgg tggatagcat
     3121 cagcatatat ctgcaaaacc aggatgtcaa cggctggaac ggagctctgg taaccatgta
     3181 ccagctggtc aagacctacg agtacaagcg gcatgaggag aggacgccgc taaacgaggc
     3241 aatgaaccta ctgttgccca tgatctacca gctgatggtc cgactgctgg cagagcagtc
     3301 cgagcaatct gtgctgctac aaaagcaaat tctcaagatc tactatgccc tcacgcaata
     3361 cacccttcca ctggacctca ttaccaagga gatcttctcg cagtggatgg agatctgtcg
     3421 tcaggtggct gatcgcgcag tgcccgatag ctcgcacctg gacgatgatg agcgaacgga
     3481 gttcccctac tggaagacta agaagtgggc cctgcacatc atggttcgca tgttcgagcg
     3541 ttatggcagt cccagcaatg tggttagtga aaaataccaa aaattcgcag agtggtatct
     3601 gcccacgttt agccagggcg tgctggaggt gctcctcaag atcctcgacc agtaccgaaa
     3661 cagagtctac gtctcaccac gcgtcctcac tgatgtgctc aactatctga agaatgcggt
     3721 tagccacgcc tacacctgga agctgattaa gcctcacatg gtggctgtca tccaggacgt
     3781 gatctttccc atcatgtcgt ttactgactc cgatcaggag ctctgggaaa gcgatcccta
     3841 cgagtacatt cgccttaagt tcgatatctt tgaggattat gccacacctg taccggcagc
     3901 ccaatctctg ctgcattcta tgtgcaaaaa gcgcaagggc attttgccca aggcaatggc
     3961 cacaattatg cagataatta catcgccgaa tgcggacaac aaacagaaag atggtgcctt
     4021 gcacatgatt ggaacgctgg cggacgtact gctgaagaag gcttcgtacc gcgaccaagt
     4081 ggagtccatg ctgaccacct acgtcttccc agaattccaa aatcccgcgg gccacatgag
     4141 agcgcgcgcg tgctgggtgc tgcactattt ctgtgacgtt caaatcaaga atccccaagt
     4201 gctggccgag ataatgcgtc tgaccacaaa cgccttgctt accgacaagg agctgcccgt
     4261 caaagtggag gcagctatcg gtctgcagat gttcatatcg tcccaggacg aggcgccgca
     4321 atacgtggag gcacagatca aggagatcac caaggagctg cttaccatca ttcgagagac
     4381 ggagaacgaa gatctaacaa acgttatgca aaagatcgtg tgcaccttca ccgagcaatt
     4441 gctgcccgtg gccaccgaga tctgccagca cctggccacc accttcagcc aggtcctaga
     4501 atctgaggag ggatccgacg agaaggccat cacggccatg agtctgctta acactatcga
     4561 aactctgctc agtgtgatgg aagagcatcc tgatgtcctg cttaacttgc acccgatcgt
     4621 cattaatgtt gtcggacaca ttttccagca caacataact gacttctatg aggaaacctt
     4681 ctccctggtc tacgacctga cagccaaggc catttccccc gaaatgtggc agatgctgga
     4741 gctcatctat caggtgttca agaaggacgg tattgactac tttatcgaca ttatgcctgc
     4801 tctgcataac tatgtgacgg tcgacacgcc cgctttcctc tccaatccca acaggctgtt
     4861 ggcgattctc gacatgtgca aaacgatgct tactagtagc cctggcgagg atcccgagtg
     4921 ccatgccgcc aaactgatgg aagtgattat cttgcagtgc aagggtcaaa tcgactcagt
     4981 gatacatatg ttcgtggagc tggctctgtc ccggttaaca cgtgaagttc aatcctcaga
     5041 gctgcgcact atgtgcctgc aagtggtaat cgcggcactc tactataatc cccagttgct
     5101 gctgtccatt ctggacaaaa tgtcccagca aaacaacgac tctatcagcg cgcactttat
     5161 caagcagtgg cttcacgaca cggactgttt cctaggcatc cacgatcgca aactttgcgt
     5221 cctgggtctg tgcaccctca tctcgctggg cgaagccaag ccccaggttc tgagcgaagt
     5281 ggctggaaag attgtgcccg cacttatttt gcttttcgat ggtctaaaac gcgcctacga
     5341 gtctcgtgct caggaggaag aggaagatga ggaggaggag gacggtgacg actgtgagga
     5401 ggctctgtcc agtgacgagg atgacatgga cgaaatggca ccggactatc tggataaatt
     5461 ggccgaattt gccaaaacca agggcaatga gtcgggcttc gaagtgaaag ccgaaatcaa
     5521 ggacgacgat gctgactcgg acggtgacgc cgaggagtct gtgggtgatc tgaacgagac
     5581 tggactggag tcgtttacca cgcccatcga cgatgaggag aacgaaagcg ccatcgatga
     5641 gtactggacg tttaaggaag ttattactgc actttctgca caagaccaag cctggtatgc
     5701 gctgttgacc tccaacctca cacctgagca agccaaggcg ctgcaagagg tggtggtgac
     5761 ggctgaccaa cgcaaagccg ccaaggaatc gaagctgatc gagaagcaag gtggcttcgc
     5821 ctttccgcag actgccgtgc ccacgtcgtt taagttcggc tcctaagcgg ccgctcgagt
     5881 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     5941 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     6001 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
     6061 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     6121 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     6181 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     6241 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt
     6301 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     6361 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     6421 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     6481 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     6541 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     6601 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     6661 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     6721 acagcttcaa ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa
     6781 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6841 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6901 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6961 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     7021 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7081 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7141 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7201 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7261 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7321 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7381 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7441 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7501 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7561 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7621 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7681 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7741 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7801 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7861 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7921 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7981 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8041 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8101 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8161 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8221 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8281 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8341 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8401 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8461 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8521 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8581 aacctataaa aataggcgta tcacgaggcc ctttcgt
//
