LOCUS       sequence-288817-        8610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1_574-1613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmXRN1_574-1613_G
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5895..5936
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5946..5963
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5986..6003)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6036..6170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6060..6079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6114..6133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6792..7380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6872..6891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7551..8342,8343..8411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8174..8193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8412..8516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8584..8602
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcgacacgg
     2761 tcccatgtat gtgtacaaat acagtacggt ggcgcagggt cccatgccag cgtatccacc
     2821 gctgagggct cttcccgtgc tctactgcac ggaggtggcc aagtggtcgc acgaaatcgc
     2881 agtcaatctg ccgtacagcg tgtgcatcga gctaccaaat gcggcgagaa ccgttttctt
     2941 cccgggattt cccaccatgc agcatctgcc cttcgatttc gagctgcgca acgaccgcgt
     3001 caaggtgttc gaacaggtca gtcgcaacca gaacattgtg ctaaaaccac gcaagcgcca
     3061 gctggaggac acactgacgg cggtggcctc ccagtacctc gggaaggtga tccacgtcgg
     3121 ctggccgcat ctcgtcaagg cgatagtggt gcgcgtggcc acgcgagatc agcgcgtcga
     3181 cagcgaggga atcacgctaa acgattcgcg gcgtttcgac tcggaatgca aggcactgca
     3241 ggaacacttt atcaatcgta tgggaataca gtttgccaac tatgatgtgc tcgtctatgt
     3301 gcgcaccttt gccggcaatt cgacggagtt ccgcgacaag ggggcattga tggtgcgcga
     3361 ctcgtggagc agctccgtca ccggctatcc agcccagggc gtggtggccg atctgactgt
     3421 gtgggaaaga atgcgcaaga atttcctcaa cgtggagcac tacttccccg ttggcagcac
     3481 gatcttcttg atcacggatc cgtattacgg cagcgaggga actgtgcagg acccacggct
     3541 ggcctacacc aacggccgga ttcaagtcag catcatggtg cgaccggaac cgaaagtaaa
     3601 tgcggcacgt cagctgcagg aagagcggga ccgggactat ctgagcacct tccaggtctg
     3661 caacttgctc cgaatcagtg gccgcaccct gggacgccta agtggcaccg tgtgggtggt
     3721 gctcggtccg cggcgtcaga agatggaaaa cgtgaccaag cacaacattg gcctgcagct
     3781 caagtatccg cggcagaacg aggagcgtgc cggatactgc ttccgcacga acaaccagtg
     3841 gtactactcc agcctggccg tggatctgat gcgtaactat tgccagcgtt acccagacgt
     3901 tatcgacttc tttggcgact ccaacgatcg cgccgagttc gtattcgaac aggacgtgtt
     3961 ccccaatgcg gttggccatc gccgcgtcga ggaactggcc aactgggtgc gccagcagcc
     4021 gcacatgaaa gtggagcgca tctcttgcgg ctccaagacg gtctgccgcg agaccatcga
     4081 actgctgata gccgccgtcg acgacctgcg ctcactgccc gtcaagcacg ttaaactgca
     4141 ggtgaagccg cacttgctca tcaagcccaa tgtcacactg ccagatgtct accgatcaaa
     4201 gcgcccggtg cgtctcttcg atcgcgtcgt aatcgtacgc accatttaca tggtgcccgt
     4261 gggcactaag ggcaccgtga ttggcattca tcccgttacg gaccccaacc cggtccgcct
     4321 ggagtgcgtc catgctgtcg acacgttctg caaggtgctg ttcgacagcc ccgttccgaa
     4381 ctgcaacaac atccatggca tcgccgaaga ccgagtttac aaggtgcccg aaattgccct
     4441 ggtgatcatc aagacggatg aggaaggaaa gaagcagaac gactgcgaac tgccggtgag
     4501 ggatcctcag ccgaaccagg cacaggacga gccagtccgc gcgaccagca gtcgctatgt
     4561 gaccgccgca gggtccacct cggtcccgat cacgatgaag acccaaatta gcgacgaatt
     4621 cgtcaagacg cgtagcgatc cgatagcccg tacggattcg tacaagccat cgtcggagcc
     4681 aaaaccggtg ccggtgccgg agcagattac caactggcgg gaacgcgtca gtacgccaac
     4741 aaacaaaccg cagcctgcgc caaataattg gcgcataaat cggtcttcat cacgtcaaca
     4801 gggcggtagt atttttgttg ctccgcccac aaaaactcct gatgcagctg caagcactgc
     4861 ttccactgca ttcaccgcag cctcgtccgc aacgctgact cctctggatc agaccttggc
     4921 gctcatgtcg gttttgggcg tgggtgagga ccagagctcg ccgccactcc aggaggcggt
     4981 gcagcagcaa cgtccgccac tacttcaaca gcaaagagct ccgtttcccg gacaaatgcc
     5041 caatctaccc aagccaccgc tcttctggca gcaagaggcg cagaagcagg aggcgctgca
     5101 gcaggaggcg cagcagcagg aggcgcagaa gaagcagcag caggcgcatg cgcaaatgga
     5161 gcctgaacgt atcaatagcc agcatttcta cagaagcggc cagacaggag cggcgttgaa
     5221 tcagccgcca ttgggtgctc catcaaaaag gcagtggcat gagtgggtgc acccgcgcat
     5281 gcagcacgcc aacgcattcc acgctggcgt caacaatgga tatcagatgc gtcccaaaaa
     5341 gaatatagct gcgcagagca cctttaacaa caacgtgcac atgcacctcc agcaaccata
     5401 ttacccgaat cagcagcagc agcagcagca gcagcagcca ctccagctga cggagatcaa
     5461 caatgcgcca ccgcgatact caacgatcca agattttgtg cccatacagg cgtaccgtcc
     5521 caagaaatta aatcgcgtcc aaccagccgg tcgtcaggac gtcgatgcca ccaagaatcc
     5581 tagtcgttcg cctgtgttgc agcaaccgac gaacgagact atagacacaa aggccagttc
     5641 tagtcttccg gtacaatcag caggcgaaca ggtgattgga ctgatgcaaa cgcttgaaat
     5701 taagccggct gcgtcgcagt ccgaatctga tggggtctca acaggcagtg cgaacgcgcc
     5761 cacagcaacg acatcatccc aggcggtcaa ccgaaggaag catcgagtgc ctcgcatcgg
     5821 agccaagttc gacttggaat acatactgcc cgactccccg catccaactt aatctagagg
     5881 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     5941 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     6001 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     6061 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6121 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6181 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     6241 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     6301 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     6361 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     6421 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     6481 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     6541 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     6601 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     6661 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     6721 caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa aaaggccgcg
     6781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6961 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     7021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7201 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7861 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8161 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8581 aaaaataggc gtatcacgag gccctttcgt
//
