LOCUS       sequence-288818-       10626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6Biso1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10626)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsTNRC6Biso1_G
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7911..7952
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7962..7979
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8002..8019)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8052..8186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8076..8095)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8130..8149
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8808..9396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8888..8907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9567..10358,10359..10427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10190..10209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10428..10532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10600..10618
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatga gagagaagga gcaagaaagg gaagaacagt taatggaaga
     2761 caagaaaagg aagaaagagg ataaaaagaa aaaagaagcc actcagaagg tcacggaaca
     2821 aaaaaccaaa gtgcccgaag tgacgaaacc aagtttaagc caaccaacgg ccgccagccc
     2881 aattggcagc tctccatcgc caccagtcaa tggtggcaac aatgccaaaa gggtggcagt
     2941 gccgaacgga caaccgccaa gcgccgcccg ctacatgcct cgggaggtgc cgccgcgatt
     3001 ccgttgccag caggaccaca aagtgttact aaaacgtggg cagccccctc caccgtcctg
     3061 catgctcctt gggggtgggg cagggcctcc tccctgcaca gcacctggag caaacccaaa
     3121 caacgcacaa gtgacaggag cgctgctgca gagtgagagt gggactgcgc cagactcaac
     3181 ccttggaggt gctgctgctt caaattatgc aaattccact tggggctcgg gagcctcctc
     3241 caacaacggc acctccccca acccaattca catctgggac aaggtgattg tagacgggtc
     3301 tgacatggaa gagtggcctt gtattgccag caaagacact gaatcttctt ccgaaaacac
     3361 caccgataac aacagtgcct cgaaccctgg ctctgagaag agcactctgc caggaagcac
     3421 cactagtaac aaaggaaaag ggagccagtg ccagtctgca agttctggga acgaatgtaa
     3481 tcttggggtc tggaaatctg accctaaggc taaatctgtt caatcttcca actctactac
     3541 agagaacaac aatggactag gaaattggag gaatgtgagt ggtcaggata gaattggacc
     3601 tggctctggc ttcagcaact ttaacccaaa tagcaaccca tctgcctggc cagcactggt
     3661 ccaagaagga acttctagga aaggggcatt ggaaacagat aatagtaatt ccagtgcaca
     3721 ggttagcaca gtaggtcaga catccaggga acagcagtca aagatggaaa atgcgggtgt
     3781 taattttgtt gtctctggca gagaacaggc tcaaattcat aacactgatg gaccaaaaaa
     3841 tggaaacact aactccttga acttaagttc accaaacccc atggagaata agggaatgcc
     3901 ctttggaatg ggcttgggga acacctccag gagcactgat gccccttcac aaagcactgg
     3961 agatcgaaag actgggagtg ttggatcttg gggtgcagct agggggcctt ctggaactga
     4021 cacagtctct ggacaaagca attctggaaa caatgggaac aatggaaaag agagagagga
     4081 ctcctggaaa ggagcttctg ttcagaaatc aactgggtca aaaaatgact cttgggacaa
     4141 caataacagg tctacgggtg ggtcctggaa ctttggcccc caggactcta atgacaacaa
     4201 atggggtgaa gggaacaaaa tgacatctgg ggtctctcag ggagaatgga aacagccgac
     4261 tgggtctgat gagttgaaaa ttggagaatg gagtggtcca aaccaaccaa attctagcac
     4321 tggagcatgg gacaatcaaa agggccaccc cctccctgaa aaccaaggca atgcccaggc
     4381 tccctgttgg ggaagatctt ccagctccac aggaagtgaa gttggaggtc aaagcactgg
     4441 aagcaaccac aaagcaggaa gtagtgacag tcataactct ggccgtcggt cgtacaggcc
     4501 cacacatcct gattgtcagg ctgtcttgca gactcttttg agccgaactg atttggaccc
     4561 cagggtgctc tcaaacactg gctggggcca aactcaaatt aagcaggaca cagtgtggga
     4621 cattgaagag gtgccaaggc ctgaggggaa atctgacaaa ggaactgagg ggtgggagag
     4681 cgctgccaca cagaccaaga actcaggggg ctggggagat gcacccagcc aaagcaatca
     4741 aatgaagtct ggatgggggg agctctcagc ctctacagag tggaaagacc ccaagaacac
     4801 aggaggctgg aatgactaca agaacaacaa ctcttccaac tggggaggag gacgacctga
     4861 tgaaaagaca ccttcctctt ggaatgagaa tcccagcaag gatcaggggt ggggaggtgg
     4921 acgccagccc aatcaaggat ggtcttctgg aaagaatggt tggggggagg aagtcgatca
     4981 gacaaaaaac agcaattggg aaagttctgc aagtaaacct gtgtctgggt ggggtgaagg
     5041 agggcagaat gaaatcggga cttggggtaa tggtggcaat gcaagcctag cttcaaaagg
     5101 tgggtgggag gattgcaaaa gatccccagc atggaatgag acgggccgac agcccaattc
     5161 ctggaataaa caacaccaac agcagcagcc cccacagcag ccgccgccac cacaaccaga
     5221 ggcttctggt tcgtggggag gcccaccccc accacctcca ggcaacgttc gaccttccaa
     5281 ttccagctgg agcagcgggc cacagcctgc aacacctaag gatgaggaac ccagtggttg
     5341 ggaagagcca tccccacagt caattagtcg gaaaatggac attgatgatg gcacttcagc
     5401 atggggagac cctaacagtt ataactacaa gaatgtgaat ctgtgggata agaattccca
     5461 agggggccca gcacctcgag aaccaaacct gcccacccca atgaccagta aatcggcatc
     5521 agattccaaa tctatgcaag acggctgggg ggagagtgac gggccagtca caggagctcg
     5581 ccatcccagc tgggaagagg aggaggatgg aggagtctgg aacaccactg gctctcaggg
     5641 cagtgcttcc tcccacaact cagcaagctg gggacaagga ggaaagaaac aaatgaagtg
     5701 ctcactcaaa ggaggaaaca atgattcatg gatgaatcct cttgccaaac agttttcaaa
     5761 tatgggattg ctgagtcaga ctgaagataa tccaagcagc aaaatggatt tgtctgtagg
     5821 aagcctttca gataaaaaat ttgatgtgga caagcgagcg atgaatctcg gggattttaa
     5881 tgatatcatg aggaaggatc gatctgggtt ccgtccacct aattccaaag acatgggaac
     5941 cacagatagt gggccttatt ttgagaaggg cggtagtcat ggtttgtttg gaaacagcac
     6001 agcacaatcg agaggtctgc acacacccgt gcagccacta aattcttctc ccagtctccg
     6061 ggcgcaagtg cctccccagt ttatttcccc ccaggtttct gcctcaatgc tcaagcagtt
     6121 tcccaacagt ggcctgagtc caggtctttt caatgtgggg ccccagttat ctcctcaaca
     6181 aattgccatg ctgagccagc ttccacaaat tccccagttt cagttggcat gtcagcttct
     6241 cttgcagcag cagcaacagc agcagttgtt acagaaccag agaaagattt ctcaagctgt
     6301 acgccaacag caagagcagc agctggctcg aatggtgagt gcactgcagc agcagcagca
     6361 gcagcagcag aggcagccag gcatgaagca ctcgccctct catcctgttg ggcccaagcc
     6421 gcatctggac aacatggtac ccaacgcatt gaatgtgggg ctcccagacc ttcaaaccaa
     6481 agggccaata cctggatatg gttctggctt cagctctggc ggcatggact atggcatggt
     6541 tggtgggaag gaggctggaa ccgagtctcg ctttaaacag tggacctcca tgatggaggg
     6601 gctgccctct gtagccacac aggaagccaa tatgcacaaa aatggcgcta tagtggcccc
     6661 tggtaaaacc cggggagggt caccgtacaa ccagtttgat atcatccctg gtgacacact
     6721 gggtggccat acgggtcctg ctggtgatag ctggttacct gccaaatctc caccaacaaa
     6781 taaaatcgga agtaaatcca gcaatgccag ttggcctcca gaattccaac caggagtgcc
     6841 atggaaaggt atccaaaaca ttgaccctga atctgacccc tatgtcaccc caggaagtgt
     6901 gctggggggt acagccacat ctcccattgt agatactgac caccaactgc tgcgggataa
     6961 caccacaggg tctaattctt ccctcaacac ctcgctgcct tcacctggtg cctggcccta
     7021 cagtgcctct gacaactcct ttaccaacgt tcatagcact tcagcaaagt tccctgatta
     7081 caaatcaaca tggtccccag atcccatagg acacaacccc actcatctct ccaacaagat
     7141 gtggaaaaac catatttcct ccaggaacac tacaccgctg ccccgcccac ctcctggtct
     7201 gaccaacccc aaaccatcat ctccctggag cagcacagca ccccgatcag tcagggggtg
     7261 ggggacacag gactcacggc tcgcctcggc ctctacctgg agtgatggtg gctcagttcg
     7321 tcctagttac tggctggttc ttcacaatct caccccacag attgatgggt caaccttgag
     7381 aacgatctgc atgcagcatg gcccactgct gacattccat ctgaatctaa cccagggcac
     7441 tgccctgatc cgatacagca ccaaacagga ggcggccaag gcccaaactg cactgcacat
     7501 gtgtgtgttg ggaaacacta ccatccttgc tgagtttgcc actgatgatg aagtcagccg
     7561 ctttctggca caagctcagc cccctacacc tgcagcaacc ccaagtgcgc cagctgcggg
     7621 gtggcagtcg ctggagaccg gccagaacca gtcagatccc gtgggacctg ctctgaatct
     7681 ttttggtggg tccactgggc tcgggcagtg gagcagcagc gctggtggca gcagcggggc
     7741 cgatcttgct ggcgcttcat tgtgggggcc cccaaactat tcttctagct tatggggagt
     7801 cccaacggtg gaagatcccc ataggatggg cagccctgct cctttactac ctggtgacct
     7861 tctgggagga gggtcggatt caatctgatc tagagggccc gcggttcgaa ggtaagccta
     7921 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
     7981 gagtttaaac ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag
     8041 cctcgatcga ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     8101 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     8161 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     8221 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     8281 ctgattatga tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     8341 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     8401 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     8461 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     8521 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     8581 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     8641 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     8701 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     8761 agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9001 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9241 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     9901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    10201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10561 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10621 tttcgt
//
