LOCUS       sequence-288823-        7082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsEDC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7082)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsEDC3_G
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1185..1211
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2786..2907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2797..2816)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2851..2870
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3093..3548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3180..3199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3390..3411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3665..4022
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3665..3856
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3688..3708)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3873..4008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4073..4867
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4127..4146)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4737..4756
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4931..4979
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5020..5039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5139..5161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5199..5217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5285..5389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5390..5458,5459..6250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5608..5627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6421..7009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6910..6929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcaat ggcttaccca tacgatgttc
     1201 cagattacgc tatggctaca gattggctgg gaagtattgt gtccatcaat tgtggagata
     1261 gcttgggtgt ctatcaggga agagtgtcag ctgtggatca ggtcagccag accatttctc
     1321 tcacccggcc tttccataat ggagtgaagt gtcttgttcc agaagtcacc ttcagggcag
     1381 gtgacattac ggagttaaaa attctggaga taccaggacc tggagacaac caacattttg
     1441 gagaccttca tcaaacagaa ttaggcccct ctggtgctgg ctgccaagtg ggcatcaatc
     1501 agaatggcac aggcaagttt gtcaagaagc cagcctcttc cagcagtgcc cctcagaata
     1561 tccctaagag gacagatgtg aagagccagg atgttgccgt ttccccgcag cagcaacagt
     1621 gctcaaagag ctatgtcgac aggcacatgg aatccttgag tcagtccaaa agtttccgtc
     1681 gtcggcacaa ctcctggtca tctagtagca ggcacccaaa tcaggcaact cccaagaaaa
     1741 gtggtttaaa gaatggccag atgaagaata aagatgacga gtgcttcggg gatgatattg
     1801 aggagatccc agacacagat tttgattttg aagggaacct ggctcttttt gacaaggcag
     1861 ctgtgtttga ggagattgat acctatgaaa ggagaagtgg tacccgttcc cggggcatcc
     1921 caaatgaaag gcccactcgg taccgccatg atgagaacat cttggagtcc gagcccattg
     1981 tctatcgacg gatcatagtg ccccacaacg tgagcaagga gttctgcacg gactctggcc
     2041 tggttgtccc aagtatttcc tatgagctgc ataaaaagct gttgtccgtg gctgagaagc
     2101 atgggctgac ccttgagcgg agactggaga tgacaggtgt gtgtgccagt cagatggcac
     2161 tgaccctcct cggaggacct aacaggttga atcccaaaaa tgttcaccag aggcctacag
     2221 tggctctact gtgtggacct catgtgaagg gggctcaggg tatcagctgt ggaaggcacc
     2281 tagccaacca tgatgtccag gtcatccttt tcctgcccaa ttttgtcaag atgttggaat
     2341 ctatcaccaa tgagctgtcg ctcttcagca agacccaagg ccaacaagtg tctagcctca
     2401 aagatctgcc cactagccct gtggacctgg tcatcaactg cctggattgc cctgagaacg
     2461 tcttcctgcg cgatcaaccc tggtacaagg cagctgtggc ctgggccaac cagaaccggg
     2521 caccagtact cagcatagac cctcctgtgc atgaagtcga acagggcatt gatgccaaat
     2581 ggtcactggc actgggcctg cctctgccac tgggggagca cgcaggccgt atctatttgt
     2641 gcgacattgg cattccccag caggtcttcc aggaggtggg catcaactac cactcgccct
     2701 ttggctgcaa gtttgttatc ccactgcact ctgcttaggg cggccgcttc cctttagtga
     2761 gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     2821 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2881 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2941 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     3001 atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3061 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     3121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3301 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3421 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     3541 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3601 cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta
     3661 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3721 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3781 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3841 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3901 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3961 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4021 aaaagcttga ttcttctgac acaacagtct cgaacttaag gctagagcca ccatgattga
     4081 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4141 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4201 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4261 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4321 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4381 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4441 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4501 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4561 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     4621 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4681 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4741 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4801 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4861 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4921 cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     4981 atcgatagcg ataaggatcc gcgtatggtg cactctcagt acaatctgct ctgatgccgc
     5041 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5101 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5161 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5221 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5281 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5341 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5401 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5461 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5521 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5581 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5641 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5701 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5761 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5821 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5881 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5941 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6001 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6061 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6121 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6181 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6241 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6301 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6361 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat
     7081 ct
//
