LOCUS       sequence-288833-        7980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182_1-830-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182_1-830-dsRNAres_G
FEATURES             Location/Qualifiers
     source          1..7980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5265..5306
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5316..5333
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5356..5373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5406..5540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5430..5449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5484..5503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6162..6750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6242..6261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6921..7712,7713..7781))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7544..7563
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7782..7886)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7954..7972
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcgtgaagc
     2761 ccttttttcc caagatggct ggggctgtca gcatgttaac caggatacta attgggaagt
     2821 tcccagttcg ccagaaccag ccaataagga tgcacccggt ccaccaatgt ggaagccaag
     2881 cattaacaat ggtactgatc tttgggagtc caatttgaga aacggaggtc agccggccgc
     2941 acagcaagtt ccaaagccgt cgtggggtca tacaccatcc tctaacttag gtggaacatg
     3001 gggtgaggac gacgatggcg ccgatagtag tagtgtgtgg actggaggag ctgttagcaa
     3061 cgcgggatcc ggagctgcag tgggagtaaa ccaagccgga gttaatgtcg gtccaggcgg
     3121 tgttgtttcg tctggcggac ctcagtgggg acaaggtgtc gttggcgtcg gacttggatc
     3181 aactggcgga aatggttcca gtaacattac aggctcttcg ggcgttgcca cgggaagcag
     3241 tggcaattca agtaatgccg gaaatggatg gggcgatcca cgcgagattc gtccactggg
     3301 cgtcggagga tcgatggaca tccgtaacgt cgagcaccgt ggaggaaatg gatcaggcgc
     3361 cacatccagt gacccgcgtg atatccgtat gattgaccca cgcgatccaa tccggggcga
     3421 cccacgcggc atttcaggac gcctcaacgg tacgtcagag atgtggggac accacccgca
     3481 gatgtcgcac aatcaactgc agggaattaa taagatggtc ggacagagcg ttgcgacagc
     3541 tagtacgagc gttggcacgt caggatccgg aattggacca ggcggaccgg gaccaagcac
     3601 ggtttcggga aacattccga cgcaatgggg tccagcccag cccgttagtg tcggagttag
     3661 cggaccgaag gatatgtcga agcaaattag cggctgggaa gagccgtcgc ctcccccaca
     3721 acgccgtagc atcccaaatt atgacgacgg aacgtccctc tggggacaac agacacgcgt
     3781 cccggccgct agtggacatt ggaaggatat gacagactcc attggacgca gcagccacct
     3841 gatgcgcgga cagagtcaga ccggcggaat tggcattgct ggagttggaa atagcaatgt
     3901 tccagtggga gccaatccaa gtaatcctat aagcagtgta gttggacctc aagcccggat
     3961 tccatctgtg ggcggcgtac aacacaaacc agacggcggc gctatgtggg tgcattccgg
     4021 caatgtaggt ggcagaaata atgttgctgc tgttactact tggggagatg acactcatag
     4081 cgttaatgtc ggcgctccca gcagtggcag tgtatccagc aacaattggg ttgatgacaa
     4141 gtccaactca accttggcac aaaactcttg gagcgacccg gcccctgttg gagttagttg
     4201 gggcaataag caaagcaaac cgccaagcaa tagtgcttca tcaggttgga gcactgctgc
     4261 gggcgtggtg gatggggttg atctaggatc tgagtggaac acgcacgggg ggattattgg
     4321 aaaatctcag caacaacaaa aactagcggg acttaacgtg ggaatggtga acgtaattaa
     4381 cgcggagatc attaagcaaa gcaagcaata caggatcctt gtcgagaacg gctttaaaaa
     4441 ggaagatgta gagcgggcat tagtgattgc taatatgaac atcgaagagg cagccgatat
     4501 gctccgtgcc aactcatccc tatcaatgga tggttggcgt cgacatgatg agtcccttgg
     4561 atcttatgcc gaccacaata gttcaacaag cagcggtgga tttgctggtc gttacccggt
     4621 caacagtgga caaccttcaa tgtcctttcc tcataataac cttatgaata acatgggagg
     4681 taccgctgtt actggaggta acaacaatac aaacatgaca gctttacagg tgcaaaagta
     4741 tttaaatcaa gggcaacatg gtgtcgctgt tggaccgcaa gccgttggta attcttcagc
     4801 agtatctgtc ggatttggtc agaacacgtc taacgcagca gtggcaggag cagcctctgc
     4861 aaatatagca gcaaatacaa acaaccaacc gtctggtcag caaattcgca tgctaggcca
     4921 gcaaattcag ttggccattc atagtggttt catatctagt cagatattga ctcaaccgct
     4981 aactcaaaca acccttaacc ttttaaacca acttcttagc aatattaagc atctccaggc
     5041 tgcgcagcaa tcccttaccc gcgggggaaa tgtcaatcca atggcagtga atgtggctat
     5101 atctaaatac aagcagcaaa tccagaattt acagaaccag ataagtgcac aacaggctgt
     5161 gtatgtaaaa cagcaaaata tgcaaccaac ttcacaacaa caacagcccc aacaacagca
     5221 acttccttct gttcatctat aatctagagg gcccgcggtt cgaaggtaag cctatcccta
     5281 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
     5341 aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga
     5401 tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     5461 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5521 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5581 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     5641 atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     5701 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     5761 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     5821 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     5881 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     5941 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     6001 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     6061 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag
     6121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     6361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6601 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6901 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6961 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7021 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7081 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7141 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7201 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     7261 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7321 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7381 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7441 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7501 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     7561 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7621 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7681 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7741 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     7921 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
