LOCUS       sequence-288838-        8886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delDUFdelCterm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delDUFdelCterm_G
FEATURES             Location/Qualifiers
     source          1..8886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6171..6212
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6222..6239
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6262..6279)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6312..6446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6336..6355)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6390..6409
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7068..7656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7148..7167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7827..8618,8619..8687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8450..8469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8688..8792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8860..8878
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcgtgaagc
     2761 ccttttttcc caagatggct ggggctgtca gcatgttaac caggatacta attgggaagt
     2821 tcccagttcg ccagaaccag ccaataagga tgcacccggt ccaccaatgt ggaagccaag
     2881 cattaacaat ggtactgatc tttgggagtc caatttgaga aacggaggtc agccggccgc
     2941 acagcaagtt ccaaagccgt cgtggggtca tacaccatcc tctaacttag gtggaacatg
     3001 gggtgaggac gacgatggcg ccgatagtag tagtgtgtgg actggaggag ctgttagcaa
     3061 cgcgggatcc ggagctgcag tgggagtaaa ccaagccgga gttaatgtcg gtccaggcgg
     3121 tgttgtttcg tctggcggac ctcagtgggg acaaggtgtc gttggcgtcg gacttggatc
     3181 aactggaggt aacgggtcaa gcaatataac tggatcgtct ggagtcgcaa caggtagtag
     3241 cggaaactcc agcaacgctg gtaacggttg gggagaccct cgtgaaatac gccctttggg
     3301 agttggtggc tccatggata ttcgaaatgt tgaacatcgc ggcggtaacg gttctggagc
     3361 aacttcgagc gatccacgag acattcgcat gatcgatccg cgtgacccta ttcgaggaga
     3421 tccccgtgga atatctggtc gtcttaatgg gacctctgaa atgtggggtc atcatccaca
     3481 aatgtcccat aaccagttgc aaggtatcaa caaaatggtt ggtcaaagtg tagcaactgc
     3541 cagcaccagt gtcggaacat ctggctcggg catcggtcct ggaggtcccg gtcctagtac
     3601 agtatcaggc aatatcccaa cacagtgggg gcctgctcaa ccggtaagcg ttggtgtaag
     3661 tggtcccaaa gacatgtcaa aacagataag tggatgggag gaaccatcac caccgcctca
     3721 gcgtcgcagt attcctaact acgatgatgg tacatcgttg tggggtcagc aaactcgtgt
     3781 tcccgctgca agcggtcact ggaaagacat gactgattcg ataggtcgta gtagtcatct
     3841 catgcgtggc caaagccaaa cgggaggtat aggaatagcc ggcgttggaa atagcaatgt
     3901 tccagtggga gccaatccaa gtaatcctat aagcagtgta gttggacctc aagcccggat
     3961 tccatctgtg ggcggcgtac aacacaaacc agacggcggc gctatgtggg tgcattccgg
     4021 caatgtaggt ggcagaaata atgttgctgc tgttactact tggggagatg acactcatag
     4081 cgttaatgtc ggcgctccca gcagtggcag tgtatccagc aacaattggg ttgatgacaa
     4141 gtccaactca accttggcac aaaactcttg gagcgacccg gcccctgttg gagttagttg
     4201 gggcaataag caaagcaaac cgccaagcaa tagtgcttca tcaggttgga gcactgctgc
     4261 gggcgtggtg gatggggttg atctaggatc tgagtggaac acgcacgggg ggattattgg
     4321 aaaatctcag caacaacaaa aactagcggg acttaacgtg ggaatggtga acgtaattaa
     4381 cgcggagatc attaagcaaa gcaagcaata caggatcctt gtcgagaacg gctttaaaaa
     4441 ggaagatgta gagcgggcat tagtgattgc taatatgaac atcgaagagg cagccgatat
     4501 gctccgtgcc aactcatccc tatcaatgga tggttggcgt cgacatgatg agtcccttgg
     4561 atcttatgcc gaccacaata gttcaacaag cagcggtgga tttgctggtc gttacccggt
     4621 caacagtgga caaccttcaa tgtcctttcc tcataataac cttatgaata acatgggagg
     4681 taccgctgtt actggaggta acaacaatac aaacatgaca gctttacagg tgcaaaagta
     4741 tttaaatcaa gggcaacatg gtgtcgctgt tggaccgcaa gccgttggta attcttcagc
     4801 agtatctgtc ggatttggtc agaacacgtc taacgcagca gtggcaggag cagcctctgt
     4861 aaatatagca gcaaatacaa acaaccaacc gtctggtcag caaattcgca tgctaggcca
     4921 gcaaattcag ttggccattc atagtggttt catatctagt cagatattga ctcaaccgct
     4981 aactcaaaca acccttaacc ttttaaacca acttcttagc aatattaagc atctccaggc
     5041 tgcgcagcaa tcccttaccc gcgggggaaa tgtcaatcca atggcagtga atgtggctat
     5101 atctaaatac aagcagcaaa tccagaattt acagaaccag ataaatgcac aacaggctgt
     5161 gtatgtaaaa cagcaaaata tgcaaccaac ttcacaacaa caacagcccc aacaacagca
     5221 acttccttct gttcatctaa gtaactcagg caacgactat ttaagaggtc acgatgcaat
     5281 aaataatttg caaagcaact tttctgagct caatattaat aagccaagtg gatatcaagg
     5341 agcgtccaat caacaatccc gattaaatca gtggaagctt ccagtattag ataaggagat
     5401 caactctgac agtacggaat tttctcgtgc cccaggtgca acgaaacaaa atttgacggc
     5461 caacacaagc aacataaact ctttgggtct tcaaaacgat agtacatggt caactggacg
     5521 cagtattggt gacggttggc ctgatccctc atctgatgcc aataccggta aaaattcacc
     5581 gactgattta ccgccactaa gtttatcgtc gtctacatgg agttttaatc caaaccaaaa
     5641 ttatccgagt cacagttggt ctgacaatag tcaacaatgt accgccactt cggagctttg
     5701 gacaagcccg ctaaataaat catcgtctcg aggtcccccg ccaggattga ctgccaattc
     5761 aaataaatct gcaaatagta atgcgtcaac gccaacaact attaccggag gtgcgaatgg
     5821 atggttacag cctcgaagtg gcggtgttca aaccacaaac actaattgga caggtggtaa
     5881 caccacttgg ggctccagtt ggttgctttt gaaaaatcta acagcacaga ttgatggtcc
     5941 tactttgcgt acactgtgta tgcagcatgg gccccttgtc agctttcacc cgtatttgaa
     6001 ccaaggaatt gccttatgta aatatactac tcgtgaggag gcgaacaagg cgcaaatggc
     6061 gttaaacaac tgtgtcctcg ccaacaccac aatatttgct gaatctccca gcgagaacga
     6121 ggtgcaaagc attatgcagc acttataatc tagagggccc gcggttcgaa ggtaagccta
     6181 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
     6241 gagtttaaac ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag
     6301 cctcgatcga ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     6361 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6421 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     6481 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     6541 ctgattatga tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     6601 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     6661 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     6721 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     6781 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     6841 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     6901 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     6961 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     7021 agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7261 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7501 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     8161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     8461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8821 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8881 tttcgt
//
