LOCUS       sequence-288840-        8168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delQdelMGW.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delQdelMGW_G
FEATURES             Location/Qualifiers
     source          1..8168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5453..5494
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5504..5521
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5544..5561)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5594..5728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5618..5637)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5672..5691
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6350..6938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6430..6449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7109..7900,7901..7969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7732..7751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7970..8074)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8142..8160
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggaggta acgggtcaag caatataact ggatcgtctg
     3181 gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg taacggttgg ggagaccctc
     3241 gtgaaatacg ccctttggga gttggtggct ccatggatat tcgaaatgtt gaacatcgcg
     3301 gcggtaacgg ttctggagca acttcgagcg atccacgaga cattcgcatg atcgatccgc
     3361 gtgaccctat tcgaggagat ccccgtggaa tatctggtcg tcttaatggg acctctgaaa
     3421 tgtggggtca tcatccacaa atgtcccata accagttgca aggtatcaac aaaatggttg
     3481 gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc tggctcgggc atcggtcctg
     3541 gaggtcccgg tcctagtaca gtatcaggca atatcccaac acagtggggg cctgctcaac
     3601 cggtaagcgt tggtgtaagt ggtcccaaag acatgtcaaa acagataagt ggatgggagg
     3661 aaccatcacc accgcctcag cgtcgcagta ttcctaacta cgatgatggt acatcgttgt
     3721 ggggtcagca aactcgtgtt cccgctgcaa gcggtcactg gaaagacatg actgattcga
     3781 taggtcgtag tagtcatctc atgcgtggcc aaagccaaac gggaggtata ggaatagccg
     3841 gcgttggaaa tagcaatgtt ccagtgggag ccaatccaag taatcctata agcagtgtag
     3901 ttggacctca agcccggatt ccatctgtgg gcggcgtaca acacaaacca gacggcggcg
     3961 ctatgtgggt gcattccggc aatgtaggtg gcagaaataa tgttgctgct gttactactt
     4021 ggggagatga cactcatagc gttaatgtcg gcgctcccag cagtggcagt gtatccagca
     4081 acaattgggt tgatgacaag tccaactcaa ccttggcaca aaactcttgg agcgacccgg
     4141 cccctgttgg agttagttgg ggcaataagc aaagcaaacc gccaagcaat agtgcttcat
     4201 caggttggag cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca
     4261 cgcacggggg gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg
     4321 gaatggtgaa cgtaattaac gcggagatca ttaagcaaag caagcaatac aggatccttg
     4381 tcgagaacgg ctttaaaaag gaagatgtag agcgggcatt agtgattgct aatatgaaca
     4441 tcgaagaggc agccgatatg ctccgtgcca actcatccct atcaatggat ggttggcgtc
     4501 gacatgatga gtcccttgga tcttatgccg accacaatag ttcaacaagc agcggtggat
     4561 ttgctggtcg ttacccggtc aacagtggac aaccttcaat gtccttttcc agttggttgc
     4621 ttttgaaaaa tctaacagca cagattgatg gtcctacttt gcgtacactg tgtatgcagc
     4681 atgggcccct tgtcagcttt cacccgtatt tgaaccaagg aattgcctta tgtaaatata
     4741 ctactcgtga ggaggcgaac aaggcgcaaa tggcgttaaa caactgtgtc ctcgccaaca
     4801 ccacaatatt tgctgaatct cccagcgaga acgaggtgca aagcattatg cagcacttac
     4861 cacaaactcc ttcctctaca agctctagtg gaactagtgg tggcaacgtc ggaggcgtcg
     4921 gcacttcagc caataatgca aacagtggtt ctgcagcttg tctgtccgga aacaatagcg
     4981 gcaacggaaa cggcagcgcg agcggcgccg gcagcggcaa caatggcaac agtagctgca
     5041 acaacagtgc cgccgggggg ggcagcagca gcaacaacac gattaccact gtagcaaatt
     5101 cgaatcttgt tggttctagt ggctctgtct caaattcctc tggcgttact gctaactcta
     5161 gtactgtttc tgtagttagt tgtacagcga gtgggaattc cataaatggg gcaggtactg
     5221 caaacagttc tggttcaaag agtagtgcaa acaatttagc tagcggccag tctagcgctt
     5281 ctaacttaac taatagcacc aattcaacat ggcgacaaac tagccaaaac caagctcttc
     5341 aaagtcaaag caggccatca ggcagagaag ctgactttga ttatatatct ctcgtttatt
     5401 ccattgttga tgattaagcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     5461 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     5521 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     5581 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     5641 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5701 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     5761 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     5821 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     5881 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     5941 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     6001 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     6061 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     6121 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     6181 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     6241 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     6301 ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6541 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6781 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     7441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     7741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8101 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8161 cctttcgt
//
