LOCUS       sequence-288863-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGe1-R1340E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Jinek M, Eulalio A, Lingel A, Helms S, Conti E, Izaurralde E
  TITLE     The C-terminal region of Ge-1 presents conserved structural features
            required for P-body localization.
  JOURNAL   RNA. 2008 Oct . 14(10):1991-8.
  PUBMED    18755833
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Oct . 14(10):1991-8."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGe1-R1340E-V5His6_G
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      5163..5172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7436..7477
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7487..7504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7527..7544)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7577..7711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7601..7620)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7655..7674
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8333..8921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8413..8432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9092..9883,9884..9952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9715..9734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9953..10057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10125..10143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgccgca tgttaatcgc
     3361 gctcttcgcg cttgcacact tgccaatctt tcgtaagacg cctttgtcag cctcctcctc
     3421 cccgccgccg tctgtccacc ggtcgccaag gtgtggcaaa gcaagtaccg cgtaccatcc
     3481 accgccagca gacgcctgca tcagcattac tgcttcagcc attgacgcag catccgggat
     3541 gagcaacagc gccgaacagg acaagccgat tgccgccaac ggcaatgcgg cagagcccca
     3601 ggaaatagaa gtcatccatt tccaagcgca agagaagcag tgttgccgcg ttattagatc
     3661 caataagacc aaggtcctca catccggcgg cgttcacaca cgcggttcct ccaaggtcaa
     3721 gctaaagaac atcgtcgact acaagtggga gcgcaagtac tactatccgg gtcacctggt
     3781 cgctgtccac cgcgatggca agcacctggc gtacgccatt aatgtcaaca acaaagccac
     3841 cggaatggag ggcatggtgc gcgtatgcaa catagccacc agtatgcgag ccttgatcaa
     3901 gggaatgagc ggcgaggtct tagatctgca gtttgcccat acggattgtg agcgtatatt
     3961 agctgtgatc gatgtcagtt cgctgtttgt ctacaaagtc gatcaaatcg aagggaacct
     4021 gctgtgcaat ctagtgctaa aggtggagga ccccatcgcc aactatgtgc cggagtacga
     4081 tatggtctcc tggtgtccct atgtctgcag cagcagcgcc accgttccaa tcaacgacga
     4141 tgacgatgag aatcagctgc tcatttggtc gcgaagctct caatttcaat gctttcaagt
     4201 gaagatgatt gtctctgaac atgggcgcgg caagatccaa ccagcagccc tagaatctgg
     4261 atatctgaag attgaggagg actcgttgat cacgtgcgcc gctttgtccc cggatggcac
     4321 cactgtggct gctgcttgcg ccgatggctt ggttcgtttc taccaaattt atttgttcga
     4381 tgtgcgcaac catcgctgcc tgcacgagtg gaagccacat gatggcaaaa aagtctgctc
     4441 tctctttttt ctcgacaata tcaacaagcc tgtggaagaa tcctactggc agcacgtgat
     4501 cactacgagc gatgccaata ccgaaatcaa gctctggaac tgctcgctat ggaaatgcct
     4561 ccaaactatc aatgtggtgg ccagtccgtc atctttgcag cccagaaact ttatcgcggg
     4621 catagatcgc agtgcgaact acttggtgtt gtcttgcctg gactctctgg ccgtgtatgt
     4681 aatgcagatt ggcagtactg gcggcgcaga ctccgagaat aggagtagcg atagcgaagg
     4741 agaaggctgc gacacttcca aacgcattca gaatgttgcc gaattcaagc tgagttcggg
     4801 gattctctct ttttctattg taaacgctag tatgcgtcgc gttaagaact ccatcgagag
     4861 ctactatcct attgaggagc ccgatgactt cgatgacgat tccaattcca ccagtgccct
     4921 tgtgctgcac atgtttgtgg tgcaggcaaa gagcctgcag gaatgtcaaa taatctacca
     4981 gccatgtgtg gctgaaaaga cggagcggag cagcctaaac agcaagcgat cacagacgcc
     5041 agaagacaat ctgctaatca aagaggagcc ggaatccccc aacagtggaa ctgtgggtgc
     5101 tgttcagctg gatgcattat tcgcaaaatc cgcaaagaga gcttcaacgg gcagttcatc
     5161 tgccatggtg gccgtggcgg cagccgcggc tgcggcacct tcggccatcc tgcaggacgc
     5221 cactaaggag gcggccaagt cagagtcccc gcaactaagc agcgcgtata cgcagcaggt
     5281 taatctcatg actccagatg ccttcagtgc aagcggaacc gcagcggcag cagctgtatt
     5341 tgtgtctacc agcaccacaa cttcaatagg aactgatagc tctaccacca ccagcggtca
     5401 ggatagatcc attgattcag cggttctgca aactatacga atgctggcca cggtaacgtc
     5461 caagacttcc gaaaacccca atgccgaggt actcctcaac ctaatgaaca acaccctaat
     5521 cgaagatcgc gagcaacaaa agctgaagga aaagcttgac gctcgcaaga agttcattgc
     5581 aattgatcgc aatccagagc ggaatgtggc tgagaacttg gccagcggca gttcgagtcc
     5641 gagtcgagag gtgcaggaga taatggccac acaggacgat gccgatgcct acgaagcaga
     5701 gctagagaat ctggacgacg atgatgacga tgaggaagaa gagctggcaa attcatcacc
     5761 gctcccggaa gctgttgacg gaacatggcc cattgtcaag ctgtcatccc actcagctga
     5821 gctccagaat gctgcccaga tcatgtccca ggcggtgcag aacaccaaca atggcaatgt
     5881 gccgcctacg ctaggtggag ggcacaataa taacacaagt gtaggcagca atagcaacaa
     5941 caacacggct acaactctta gcaccagcaa caccagcagc agcaacaacg cgggcggaac
     6001 atgcgttgat tcaagtggaa ctggagagct aaatgccaaa atggaactac tcatagatct
     6061 agtaaaagcc cagtccaaac aaataaacaa attggagaac gaagtcaaca agctacaaaa
     6121 gcagcaagaa gcggcggcgg ccctgcatag caaacaagac acttcattgg agcctaaaaa
     6181 tcttagtcag ctagcatata aaattgagat gcaactgtcc aagctaatgg agcagtatct
     6241 caagcgatat gaaaacgagc ataagaagaa actgacggaa ttcttggcgg cgcgtgagag
     6301 ccaaaaccga gagctacgtg actccgtgct gcaggtacta aatcagtatg tcatgaacca
     6361 ctttacggac attattggca acgttctcaa catggaactg cagcgccaac tgcttcctcg
     6421 cgtgaatgcc aacatggatc agctccaagc ccaaatgcaa gtggagattg tgcagaagct
     6481 gagtgtgttt gacaaaacgg tgaaggagaa tattgcccag gtgtgcaaga gcaagcaatt
     6541 tctggatact tttggcaagt ccgtgttgat tggtgttcaa accagcctac aaaccgcctt
     6601 cattgaatct atgagcagca ccttgattcc agcttatgag aagtcctcgc agaacatgtt
     6661 taaacaactg catgacgctt tcagcgtggg cataaaagac tttatggtcc agtttaacac
     6721 ctacctgcag cacatgccac agccccaagc cgggagtggc aatacggagg agataaacaa
     6781 caagttgtcc atgcttaagc aattggtaga gagcagcttg cacaagcacc gcactgaact
     6841 cacggacgcc atgctcgaga cacaaagaga agtgaagagc ttagagattt tgctggcgcg
     6901 tcaggtacag gaaacgatca gagcagagct acgcaagcac atggaagccc agaacgtggc
     6961 aatgcgttcg caggcagcta cgcctgcacc accttacgac ctcagggaca gcattaagca
     7021 gcttttgatg gctggacaaa tcaacaaggc cttccatcag gctctattgg ccaacgactt
     7081 gggcctagtg gagttcactc tccgacacac ggacagcaac caggcattcg caccagaggg
     7141 atgcagactg gagcaaaagg ttctactgtc gctcattcag caaatatccg ctgacatgac
     7201 caatcacaat gagcttaagc agagatatct caacgaggca ctgttggcca tcaatatggc
     7261 agatcccatt acccgcgagc acgctcctaa agtgctcacc gagctgtatc gcaattgtca
     7321 gcagttcatt aagaacagcc ccaagaattc gcagttcagc aacgtcgagc tgctgatgaa
     7381 ggcaatcata acgtaccgcg atcagctaaa aagtctagag ggcccgcggt tcgaaggtaa
     7441 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     7501 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg
     7561 atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     7621 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     7681 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     7741 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     7801 tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     7861 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     7921 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     7981 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     8041 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     8101 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     8161 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     8221 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     8281 ttaccagcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8521 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8761 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     9421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     9721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10081 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    10141 ggccctttcg t
//
