LOCUS       sequence-28887-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Pera EM, Ikeda A, Eivers E, De Robertis EM
  TITLE     Integration of IGF, FGF, and anti-BMP signals via Smad1 
            phosphorylation in neural induction.
  JOURNAL   Genes Dev. 2003 Dec 15. 17(24):3023-8.
  PUBMED    14701872
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2003 Dec 15. 17(24):3023-8."
COMMENT     Sequence Label: pCS2 hSmad1-MM
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    394..528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(418..437)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     472..491
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(641..661)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(641..659)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(680..696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(693..715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    704..720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtcggactgc aactaccatc atggatttca tcctactact gtttgcaaga tccctagtgg
       61 gtgtagtctg aaaattttta acaaccaaga atttgctcag ttattggcac agtctgtgaa
      121 ccatggattt gagacagtct atgagcttac aaaaatgtgt actatacgta tgagctttgt
      181 gaagggctgg ggagcagaat accaccgcca ggatgttact agcaccccct gctggattga
      241 gatacatctg cacggccccc tccagtggct ggataaagtt cttactcaaa tgggttcacc
      301 tcataatcct atttcatctg tatcttaagg atcccatcga ttcgaattca aggcctctcg
      361 agcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
      421 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      481 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      541 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
      601 gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
      661 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      721 ttccacacaa catacgagcc gggagcataa a
//
