LOCUS       sequence-288875-        9161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delDUFdelRBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delDUFdelRBD_G
FEATURES             Location/Qualifiers
     source          1..9161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6446..6487
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6497..6514
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6537..6554)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6587..6721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6611..6630)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6665..6684
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7343..7931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7423..7442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8102..8893,8894..8962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8725..8744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8963..9067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9135..9153
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggaggta acgggtcaag caatataact ggatcgtctg
     3181 gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg taacggttgg ggagaccctc
     3241 gtgaaatacg ccctttggga gttggtggct ccatggatat tcgaaatgtt gaacatcgcg
     3301 gcggtaacgg ttctggagca acttcgagcg atccacgaga cattcgcatg atcgatccgc
     3361 gtgaccctat tcgaggagat ccccgtggaa tatctggtcg tcttaatggg acctctgaaa
     3421 tgtggggtca tcatccacaa atgtcccata accagttgca aggtatcaac aaaatggttg
     3481 gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc tggctcgggc atcggtcctg
     3541 gaggtcccgg tcctagtaca gtatcaggca atatcccaac acagtggggg cctgctcaac
     3601 cggtaagcgt tggtgtaagt ggtcccaaag acatgtcaaa acagataagt ggatgggagg
     3661 aaccatcacc accgcctcag cgtcgcagta ttcctaacta cgatgatggt acatcgttgt
     3721 ggggtcagca aactcgtgtt cccgctgcaa gcggtcactg gaaagacatg actgattcga
     3781 taggtcgtag tagtcatctc atgcgtggcc aaagccaaac gggaggtata ggaatagccg
     3841 gcgttggaaa tagcaatgtt ccagtgggag ccaatccaag taatcctata agcagtgtag
     3901 ttggacctca agcccggatt ccatctgtgg gcggcgtaca acacaaacca gacggcggcg
     3961 ctatgtgggt gcattccggc aatgtaggtg gcagaaataa tgttgctgct gttactactt
     4021 ggggagatga cactcatagc gttaatgtcg gcgctcccag cagtggcagt gtatccagca
     4081 acaattgggt tgatgacaag tccaactcaa ccttggcaca aaactcttgg agcgacccgg
     4141 cccctgttgg agttagttgg ggcaataagc aaagcaaacc gccaagcaat agtgcttcat
     4201 caggttggag cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca
     4261 cgcacggggg gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg
     4321 gaatggtgaa cgtaattaac gcggagatca ttaagcaaag caagcaatac aggatccttg
     4381 tcgagaacgg ctttaaaaag gaagatgtag agcgggcatt agtgattgct aatatgaaca
     4441 tcgaagaggc agccgatatg ctccgtgcca actcatccct atcaatggat ggttggcgtc
     4501 gacatgatga gtcccttgga tcttatgccg accacaatag ttcaacaagc agcggtggat
     4561 ttgctggtcg ttacccggtc aacagtggac aaccttcaat gtcctttcct cataataacc
     4621 ttatgaataa catgggaggt accgctgtta ctggaggtaa caacaataca aacatgacag
     4681 ctttacaggt gcaaaagtat ttaaatcaag ggcaacatgg tgtcgctgtt ggaccgcaag
     4741 ccgttggtaa ttcttcagca gtatctgtcg gatttggtca gaacacgtct aacgcagcag
     4801 tggcaggagc agcctctgta aatatagcag caaatacaaa caaccaaccg tctggtcagc
     4861 aaattcgcat gctaggccag caaattcagt tggccattca tagtggtttc atatctagtc
     4921 agatattgac tcaaccgcta actcaaacaa cccttaacct tttaaaccaa cttcttagca
     4981 atattaagca tctccaggct gcgcagcaat cccttacccg cgggggaaat gtcaatccaa
     5041 tggcagtgaa tgtggctata tctaaataca agcagcaaat ccagaattta cagaaccaga
     5101 taaatgcaca acaggctgtg tatgtaaaac agcaaaatat gcaaccaact tcacaacaac
     5161 aacagcccca acaacagcaa cttccttctg ttcatctaag taactcaggc aacgactatt
     5221 taagaggtca cgatgcaata aataatttgc aaagcaactt ttctgagctc aatattaata
     5281 agccaagtgg atatcaagga gcgtccaatc aacaatcccg attaaatcag tggaagcttc
     5341 cagtattaga taaggagatc aactctgaca gtacggaatt ttctcgtgcc ccaggtgcaa
     5401 cgaaacaaaa tttgacggcc aacacaagca acataaactc tttgggtctt caaaacgata
     5461 gtacatggtc aactggacgc agtattggtg acggttggcc tgatccctca tctgatgcca
     5521 ataccggtaa aaattcaccg actgatttac cgccactaag tttatcgtcg tctacatgga
     5581 gttttaatcc aaaccaaaat tatccgagtc acagttggtc tgacaatagt caacaatgta
     5641 ccgccacttc ggagctttgg acaagcccgc taaataaatc atcgtctcga ggtcccccgc
     5701 caggattgac tgccaattca aataaatctg caaatagtaa tgcgtcaacg ccaacaacta
     5761 ttaccggagg tgcgaatgga tggttacagc ctcgaagtgg cggtgttcaa accacaaaca
     5821 ctaattggac aggtggtaac accacttggg gcccacaaac tccttcctct acaagctcta
     5881 gtggaactag tggtggcaac gtcggaggcg tcggcacttc agccaataat gcaaacagtg
     5941 gttctgcagc ttgtctgtcc ggaaacaata gcggcaacgg aaacggcagc gcgagcggcg
     6001 ccggcagcgg caacaatggc aacagtagct gcaacaacag tgccgccggg gggggcagca
     6061 gcagcaacaa cacgattacc actgtagcaa attcgaatct tgttggttct agtggctctg
     6121 tctcaaattc ctctggcgtt actgctaact ctagtactgt ttctgtagtt agttgtacag
     6181 cgagtgggaa ttccataaat ggggcaggta ctgcaaacag ttctggttca aagagtagtg
     6241 caaacaattt agctagcggc cagtctagcg cttctaactt aactaatagc accaattcaa
     6301 catggcgaca aactagccaa aaccaagctc ttcaaagtca aagcaggcca tcaggcagag
     6361 aagctgactt tgattatata tctctcgttt attccattgt tgatgattaa gcggccgctc
     6421 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     6481 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     6541 gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat
     6601 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6661 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6721 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6781 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc
     6841 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     6901 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     6961 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     7021 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     7081 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     7141 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     7201 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     7261 agggacagct tcaaggatcg ctcgcggctc ttaccagcca gcaaaaggcc aggaaccgta
     7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7501 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8401 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8701 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9061 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9121 cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
