LOCUS       sequence-288879-        9055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO1-F2V2-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-EGFP-HA-DmAGO1-F2V2-siRNAres_G
FEATURES             Location/Qualifiers
     source          1..9055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3323..3349
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6340..6381
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6391..6408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6431..6448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6481..6615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6505..6524)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6559..6578
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7237..7825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7317..7336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7996..8787,8788..8856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8619..8638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8857..8961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9029..9047
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattcta tgtacccata cgatgttcca gattacgcta tgtccacgga
     3361 gcgtgagctg gctcccggtg ggccagctca gctccatccg cacacgctgc cgctgacttt
     3421 tccggatctc cagatgacct ccaccgtggg catcattggg aaagtctacg agtcacagtg
     3481 gaccccctcg cccactcggc ctcagagtcc ctctcaggcg cagactagct tcgacacgct
     3541 cacatcacca ccagcgcccg gctcgtcggt caatcccacc gccgtcacca gcccaagtgc
     3601 ccagaatgtg gccgctggtg gagcaactgt ggccggtgca gcagcaactg ccgcccaggt
     3661 ggcctccgcc ttgggtgcca ccaccggcag cgtgacgcca gcaattgcca ccgcaacgcc
     3721 agccacgcag ccggatatgc ccgtctttac gtgtccacga cgcccgaatc tcggacgaga
     3781 gggtcgcccg attgtgctgc gcgccaatca cttccaggtg acaatgccgc gtggctatgt
     3841 gcatcactat gacatcaata tacagccgga caagtgtccg cgaaaggtga accgtgagat
     3901 tatcgagact atggtgcatg cctatagcaa gatcttcgga gtgctcaagc cggtgttcga
     3961 tggtcgcaac aatctgtata cccgcgatcc cctgcccatt ggcaacgagc gtctagagct
     4021 ggaggttact ctacccggcg agggcaaaga tcgaatcttt cgcgtgacga tcaagtggca
     4081 ggctcaggtc tcgctcttca atttggagga agctctcgaa ggccgcacgc ggcagatacc
     4141 ctatgatgcc attttggcgc tcgatgtggt catgcgccat ctgcccagca tgacgtacac
     4201 gccagtggga cgtagcttct tcagttcccc ggagggttac taccatcccc tgggtggtgg
     4261 acgcgaggtt tggttcggtt tccatcagag cgcaaggccc tcgcagtgga agatgatgct
     4321 caatatcgat gtctcggcca ccgctttcta caaggctcaa ccagtcattg acttcatgtg
     4381 cgaggtgctg gacattcgcg acatcaacga gcagcgcaaa ccgctcaccg attcgcagcg
     4441 cgtcaagttc accaaagaaa ttaaaggcct gaagatcgag atcacccact gcggccagat
     4501 gcgtcgcaag tatcgtgtgt gcaacgtcac tcgccgcccc gctcagatgc aatcattccc
     4561 actgcagctg gagaacggac agaccgtaga gtgcaccgtg gccaagtact tcctggacaa
     4621 gtaccgcatg aaattgcgct acccgcactt gccctgcctg caggttggcc aagagcacaa
     4681 gcacacttac ctgcctctag aggtgtgcaa cattgtggcc ggacagcggt gcattaaaaa
     4741 gctgaccgat atgcagacgt cgaccatgat caaggccaca gctcgttcag ctccggatcg
     4801 tgagcgtgag attaacaact tggtaaagcg cgccgacttc aacaacgatt cgtatgtgca
     4861 agagtttggc ctgaccatct ccaattcgat gatggaggta cgaggacgcg tcttgcctcc
     4921 tcccaagctt cagtatgggg gacgtgtgtc taccggcctc accggccagc agctgttccc
     4981 gccacagaac aaggtgagct tggcctcgcc caaccagggt gtatgggata tgcgaggcaa
     5041 gcagttcttc actggcgtcg agatccgcat ctgggccatc gcctgttttg ccccacagcg
     5101 cacggtgcgc gaggatgcgc tgcgtaatgt cacccagcag ctgcagaaga tctcaaacga
     5161 tgcaggcatg ccgataattg gacagccgtg cttctgtaag tacgccaccg ggccggatca
     5221 agtggaaccc atggtccgtt acctgaagat caccttcccc ggcctgcaac tcgtcgtggt
     5281 tgtgctgccc ggcaagactc cagtatacgc cgaggtgaag cgtgtaggtg acaccgttct
     5341 gggtatggcc acccagtgtg tgcaggccaa gaacgtgaac aagacatcgc cacagacgct
     5401 ctctaatctg tgtctgaaga tcaacgtcaa gttgggcggc atcaattcaa ttctggtccc
     5461 ctccattcgg ccaaaggtct tcaatgagcc ggtcatcttt ttgggtgccg atgtgacaca
     5521 cccaccagct ggcgacaaca agaaaccatc gattgccgcc gtcgtgggct ccatggatgc
     5581 ccatccatcg cggtatgccg ccaccgttcg cgtacagcag caccgacagg agatcatcca
     5641 ggagctgagc agcatggtgc gcgagctgtt gatcatgttc tacaagtcga cgggcggcta
     5701 caagccccac cgcatcatac tctatcgtga cggagtctcc gagggacaat tcccacatgt
     5761 cctgcaacac gaattgaccg ccattcgcga ggcctgcatt aagctagagc cagagtatcg
     5821 gccgggcatc acattcattg tggtgcagaa gcgccatcac actcgactct tctgcgcgga
     5881 gaagaaggag cagagcggca aatcgggcaa tattcccgca ggcaccaccg tcgatgtggg
     5941 catcacacat cccaccgaat ttgatttcta tctatgcagc catcagggca tccagggcac
     6001 cagtcgcccc tcgcactacc acgttctgtg ggacgacaat cactttgact cggacgagct
     6061 gcagtgcctc acgtatcagc tatgccgtac gtacgtgcgc tgcacccgat ccgtcagtat
     6121 accggcgcca gcctactacg cccatttagt ggccttccgt gccagatatc atctggtgga
     6181 gaaggagcac gattcgggcg agggttcgca ccagagcggc tgctcagagg atcgtacgcc
     6241 aggtgccatg gccagggcca tcactgtgca cgcggatacc aagaaggtca tgtactttgc
     6301 ctaagcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     6361 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
     6421 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
     6481 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6541 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6601 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6661 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     6721 cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     6781 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     6841 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     6901 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     6961 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     7021 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     7081 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     7141 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa
     7201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     7441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7861 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8281 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     8341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8581 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     8641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9001 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgt
//
