LOCUS       sequence-288882-       10038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delDUF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10038)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGW182-delDUF_H
FEATURES             Location/Qualifiers
     source          1..10038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7323..7364
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7374..7391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7414..7431)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7464..7598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7488..7507)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7542..7561
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8220..8808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8300..8319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8979..9770,9771..9839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9602..9621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9840..9944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10012..10030
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgcatgc gtgaagccct
     3361 tttttcccaa gatggctggg gctgtcagca tgttaaccag gatactaatt gggaagttcc
     3421 cagttcgcca gaaccagcca ataaggatgc acccggtcca ccaatgtgga agccaagcat
     3481 taacaatggt actgatcttt gggagtccaa tttgagaaac ggaggtcagc cggccgcaca
     3541 gcaagttcca aagccgtcgt ggggtcatac accatcctct aacttaggtg gaacatgggg
     3601 tgaggacgac gatggcgccg atagtagtag tgtgtggact ggaggagctg ttagcaacgc
     3661 gggatccgga gctgcagtgg gagtaaacca agccggagtt aatgtcggtc caggcggtgt
     3721 tgtttcgtct ggcggacctc agtggggaca aggtgtcgtt ggcgtcggac ttggatcaac
     3781 tggaggtaac gggtcaagca atataactgg atcgtctgga gtcgcaacag gtagtagcgg
     3841 aaactccagc aacgctggta acggttgggg agaccctcgt gaaatacgcc ctttgggagt
     3901 tggtggctcc atggatattc gaaatgttga acatcgcggc ggtaacggtt ctggagcaac
     3961 ttcgagcgat ccacgagaca ttcgcatgat cgatccgcgt gaccctattc gaggagatcc
     4021 ccgtggaata tctggtcgtc ttaatgggac ctctgaaatg tggggtcatc atccacaaat
     4081 gtcccataac cagttgcaag gtatcaacaa aatggttggt caaagtgtag caactgccag
     4141 caccagtgtc ggaacatctg gctcgggcat cggtcctgga ggtcccggtc ctagtacagt
     4201 atcaggcaat atcccaacac agtgggggcc tgctcaaccg gtaagcgttg gtgtaagtgg
     4261 tcccaaagac atgtcaaaac agataagtgg atgggaggaa ccatcaccac cgcctcagcg
     4321 tcgcagtatt cctaactacg atgatggtac atcgttgtgg ggtcagcaaa ctcgtgttcc
     4381 cgctgcaagc ggtcactgga aagacatgac tgattcgata ggtcgtagta gtcatctcat
     4441 gcgtggccaa agccaaacgg gaggtatagg aatagccggc gttggaaata gcaatgttcc
     4501 agtgggagcc aatccaagta atcctataag cagtgtagtt ggacctcaag cccggattcc
     4561 atctgtgggc ggcgtacaac acaaaccaga cggcggcgct atgtgggtgc attccggcaa
     4621 tgtaggtggc agaaataatg ttgctgctgt tactacttgg ggagatgaca ctcatagcgt
     4681 taatgtcggc gctcccagca gtggcagtgt atccagcaac aattgggttg atgacaagtc
     4741 caactcaacc ttggcacaaa actcttggag cgacccggcc cctgttggag ttagttgggg
     4801 caataagcaa agcaaaccgc caagcaatag tgcttcatca ggttggagca ctgctgcggg
     4861 cgtggtggat ggggttgatc taggatctga gtggaacacg cacgggggga ttattggaaa
     4921 atctcagcaa caacaaaaac tagcgggact taacgtggga atggtgaacg taattaacgc
     4981 ggagatcatt aagcaaagca agcaatacag gatccttgtc gagaacggct ttaaaaagga
     5041 agatgtagag cgggcattag tgattgctaa tatgaacatc gaagaggcag ccgatatgct
     5101 ccgtgccaac tcatccctat caatggatgg ttggcgtcga catgatgagt cccttggatc
     5161 ttatgccgac cacaatagtt caacaagcag cggtggattt gctggtcgtt acccggtcaa
     5221 cagtggacaa ccttcaatgt cctttcctca taataacctt atgaataaca tgggaggtac
     5281 cgctgttact ggaggtaaca acaatacaaa catgacagct ttacaggtgc aaaagtattt
     5341 aaatcaaggg caacatggtg tcgctgttgg accgcaagcc gttggtaatt cttcagcagt
     5401 atctgtcgga tttggtcaga acacgtctaa cgcagcagtg gcaggagcag cctctgcaaa
     5461 tatagcagca aatacaaaca accaaccgtc tggtcagcaa attcgcatgc taggccagca
     5521 aattcagttg gccattcata gtggtttcat atctagtcag atattgactc aaccgctaac
     5581 tcaaacaacc cttaaccttt taaaccaact tcttagcaat attaagcatc tccaggctgc
     5641 acagcaatcc cttacccgcg ggggaaatgt caatccaatg gcagtgaatg tggctatatc
     5701 taaatacaag cagcaaatcc agaatttaca gaaccagata agtgcacaac aggctgtgta
     5761 tgtaaaacag caaaatatgc aaccaacttc acaacaacaa cagccccaac aacagcaact
     5821 tccttctgtt catctaagta actcaggcaa cgactattta agaggtcacg atgcaataaa
     5881 taatttgcaa agcaactttt ctgagctcaa tattaataag ccaagtggat atcaaggagc
     5941 gtccaatcaa caatcccgat taaatcagtg gaagcttcca gtattagata aggagatcaa
     6001 ctctgacagt acggaatttt ctcgtgcccc aggtgcaacg aaacaaaatt tgacggccaa
     6061 cacaagcaac ataaactctt tgggtcttca aaacgatagt acatggtcaa ctggacgcag
     6121 tattggtgac ggttggcctg atccctcatc tgatgccaat accggtaaaa attcaccgac
     6181 tgatttaccg ccactaagtt tatcgtcgtc tacatggagt tttaatccaa accaaaatta
     6241 tccgagtcac agttggtctg acaatagtca acaatgtacc gccacttcgg agctttggac
     6301 aagcccgcta aataaatcat cgtctcgagg tcccccgcca ggattgactg ccaattcaaa
     6361 taaatctgca aatagtaatg cgtcaacgcc aacaactatt accggaggtg cgaatggatg
     6421 gttacagcct cgaagtggcg gtgttcaaac cacaaacact aattggacag gtggtaacac
     6481 cacttggggc tccagttggt tgcttttgaa aaatctaaca gcacagattg atggtcctac
     6541 tttgcgtaca ctgtgtatgc agcatgggcc ccttgtcagc tttcacccgt atttgaacca
     6601 aggaattgcc ttatgtaaat atactactcg tgaggaggcg aacaaggcgc aaatggcgtt
     6661 aaacaactgt gtcctcgcca acaccacaat atttgctgaa tctcccagcg agaacgaggt
     6721 gcaaagcatt atgcagcact taccacaaac tccttcctct acaagctcta gtggaactag
     6781 tggtggcaac gtcggaggcg tcggcacttc agccaataat gcaaacagtg gttctgcagc
     6841 ttgtctgtcc ggaaacaata gcggcaacgg aaacggcagc gcgagcggcg ccggcagcgg
     6901 caacaatggc aacagtagct gcaacaacag tgccgccggg gggggcagca gcagcaacaa
     6961 cacgattacc actgtagcaa attcgaatct tgttggttct agtggctctg tctcaaattc
     7021 ctctggcgtt actgctaact ctagtactgt ttctgtagtt agttgtacag cgagtgggaa
     7081 ttccataaat ggggcaggta ctgcaaacag ttctggttca aagagtagtg caaacaattt
     7141 agctagcggc cagtctagcg cttctaactt aactaatagc accaattcaa catggcgaca
     7201 aactagccaa aaccaagctc ttcaaagtca aagcaggcca tcaggcagag aagctgactt
     7261 tgattatata tctctcgttt attccattgt tgatgattaa tctagagggc ccgcggttcg
     7321 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     7381 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag
     7441 ctcgctgatc agcctcgatc gaggatccag acatgataag atacattgat gagtttggac
     7501 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     7561 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     7621 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     7681 aatgtggtat ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc
     7741 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     7801 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     7861 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     7921 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     7981 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     8041 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     8101 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     8161 gcggctctta ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8401 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8641 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     9301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     9601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9961 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    10021 atcacgaggc cctttcgt
//
