LOCUS       sequence-288887-       10429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6Biso1_1-1618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10429)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6Biso1_1-1618_H
FEATURES             Location/Qualifiers
     source          1..10429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6133..6254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6144..6163)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6198..6217
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6440..6895
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6527..6546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6737..6758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7012..7369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7012..7203
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7035..7055)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7220..7355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7420..8214
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7474..7493)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8084..8103
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8278..8326
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8367..8386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8486..8508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8546..8564)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8632..8736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8737..8805,8806..9597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8955..8974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9768..10356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10257..10276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcacg cgtggtacct ctagagatgg
     1201 cctacccata tgatgttcca gattacgcta tgagagagaa ggagcaagaa agggaagaac
     1261 agttaatgga agacaagaaa aggaagaaag aggataaaaa gaaaaaagaa gccactcaga
     1321 aggtcacgga acaaaaaacc aaagtgcccg aagtgacgaa accaagttta agccaaccaa
     1381 cggccgccag cccaattggc agctctccat cgccaccagt caatggtggc aacaatgcca
     1441 aaagggtggc agtgccgaac ggacaaccgc caagcgccgc ccgctacatg cctcgggagg
     1501 tgccgccgcg attccgttgc cagcaggacc acaaagtgtt actaaaacgt gggcagcccc
     1561 ctccaccgtc ctgcatgctc cttgggggtg gggcagggcc tcctccctgc acagcacctg
     1621 gagcaaaccc aaacaacgca caagtgacag gagcgctgct gcagagtgag agtgggactg
     1681 cgccagactc aacccttgga ggtgctgctg cttcaaatta tgcaaattcc acttggggct
     1741 cgggagcctc ctccaacaac ggcacctccc ccaacccaat tcacatctgg gacaaggtga
     1801 ttgtagacgg gtctgacatg gaagagtggc cttgtattgc cagcaaagac actgaatctt
     1861 cttccgaaaa caccaccgat aacaacagtg cctcgaaccc tggctctgag aagagcactc
     1921 tgccaggaag caccactagt aacaaaggaa aagggagcca gtgccagtct gcaagttctg
     1981 ggaacgaatg taatcttggg gtctggaaat ctgaccctaa ggctaaatct gttcaatctt
     2041 ccaactctac tacagagaac aacaatggac taggaaattg gaggaatgtg agtggtcagg
     2101 atagaattgg acctggctct ggcttcagca actttaaccc aaatagcaac ccatctgcct
     2161 ggccagcact ggtccaagaa ggaacttcta ggaaaggggc attggaaaca gataatagta
     2221 attccagtgc acaggttagc acagtaggtc agacatccag ggaacagcag tcaaagatgg
     2281 aaaatgcggg tgttaatttt gttgtctctg gcagagaaca ggctcaaatt cataacactg
     2341 atggaccaaa aaatggaaac actaactcct tgaacttaag ttcaccaaac cccatggaga
     2401 ataagggaat gccctttgga atgggcttgg ggaacacctc caggagcact gatgcccctt
     2461 cacaaagcac tggagatcga aagactggga gtgttggatc ttggggtgca gctagggggc
     2521 cttctggaac tgacacagtc tctggacaaa gcaattctgg aaacaatggg aacaatggaa
     2581 aagagagaga ggactcctgg aaaggagctt ctgttcagaa atcaactggg tcaaaaaatg
     2641 actcttggga caacaataac aggtctacgg gtgggtcctg gaactttggc ccccaggact
     2701 ctaatgacaa caaatggggt gaagggaaca aaatgacatc tggggtctct cagggagaat
     2761 ggaaacagcc gactgggtct gatgagttga aaattggaga atggagtggt ccaaaccaac
     2821 caaattctag cactggagca tgggacaatc aaaagggcca ccccctccct gaaaaccaag
     2881 gcaatgccca ggctccctgt tggggaagat cttccagctc cacaggaagt gaagttggag
     2941 gtcaaagcac tggaagcaac cacaaagcag gaagtagtga cagtcataac tctggccgtc
     3001 ggtcgtacag gcccacacat cctgattgtc aggctgtctt gcagactctt ttgagccgaa
     3061 ctgatttgga ccccagggtg ctctcaaaca ctggctgggg ccaaactcaa attaagcagg
     3121 acacagtgtg ggacattgaa gaggtgccaa ggcctgaggg gaaatctgac aaaggaactg
     3181 aggggtggga gagcgctgcc acacagacca agaactcagg gggctgggga gatgcaccca
     3241 gccaaagcaa tcaaatgaag tctggatggg gggagctctc agcctctaca gagtggaaag
     3301 accccaagaa cacaggaggc tggaatgact acaagaacaa caactcttcc aactggggag
     3361 gaggacgacc tgatgaaaag acaccttcct cttggaatga gaatcccagc aaggatcagg
     3421 ggtggggagg tggacgccag cccaatcaag gatggtcttc tggaaagaat ggttgggggg
     3481 aggaagtcga tcagacaaaa aacagcaatt gggaaagttc tgcaagtaaa cctgtgtctg
     3541 ggtggggtga aggagggcag aatgaaatcg ggacttgggg taatggtggc aatgcaagcc
     3601 tagcttcaaa aggtgggtgg gaggattgca aaagatcccc agcatggaat gagacgggcc
     3661 gacagcccaa ttcctggaat aaacaacacc aacagcagca gcccccacag cagccgccgc
     3721 caccacaacc agaggcttct ggttcgtggg gaggcccacc cccaccacct ccaggcaacg
     3781 ttcgaccttc caattccagc tggagcagcg ggccacagcc tgcaacacct aaggatgagg
     3841 aacccagtgg ttgggaagag ccatccccac agtcaattag tcggaaaatg gacattgatg
     3901 atggcacttc agcatgggga gaccctaaca gttataacta caagaatgtg aatctgtggg
     3961 ataagaattc ccaagggggc ccagcacctc gagaaccaaa cctgcccacc ccaatgacca
     4021 gtaaatcggc atcagattcc aaatctatgc aagacggctg gggggagagt gacgggccag
     4081 tcacaggagc tcgccatccc agctgggaag aggaggagga tggaggagtc tggaacacca
     4141 ctggctctca gggcagtgct tcctcccaca actcagcaag ctggggacaa ggaggaaaga
     4201 aacaaatgaa gtgctcactc aaaggaggaa acaatgattc atggatgaat cctcttgcca
     4261 aacagttttc aaatatggga ttgctgagtc agactgaaga taatccaagc agcaaaatgg
     4321 atttgtctgt aggaagcctt tcagataaaa aatttgatgt ggacaagcga gcgatgaatc
     4381 tcggggattt taatgatatc atgaggaagg atcgatctgg gttccgtcca cctaattcca
     4441 aagacatggg aaccacagat agtgggcctt attttgagaa gggcggtagt catggtttgt
     4501 ttggaaacag cacagcacaa tcgagaggtc tgcacacacc cgtgcagcca ctaaattctt
     4561 ctcccagtct ccgggcgcaa gtgcctcccc agtttatttc cccccaggtt tctgcctcaa
     4621 tgctcaagca gtttcccaac agtggcctga gtccaggtct tttcaatgtg gggccccagt
     4681 tatctcctca acaaattgcc atgctgagcc agcttccaca aattccccag tttcagttgg
     4741 catgtcagct tctcttgcag cagcagcaac agcagcagtt gttacagaac cagagaaaga
     4801 tttctcaagc tgtacgccaa cagcaagagc agcagctggc tcgaatggtg agtgcactgc
     4861 agcagcagca gcagcagcag cagaggcagc caggcatgaa gcactcgccc tctcatcctg
     4921 ttgggcccaa gccgcatctg gacaacatgg tacccaacgc attgaatgtg gggctcccag
     4981 accttcaaac caaagggcca atacctggat atggttctgg cttcagctct ggcggcatgg
     5041 actatggcat ggttggtggg aaggaggctg gaaccgagtc tcgctttaaa cagtggacct
     5101 ccatgatgga ggggctgccc tctgtagcca cacaggaagc caatatgcac aaaaatggcg
     5161 ctatagtggc ccctggtaaa acccggggag ggtcaccgta caaccagttt gatatcatcc
     5221 ctggtgacac actgggtggc catacgggtc ctgctggtga tagctggtta cctgccaaat
     5281 ctccaccaac aaataaaatc ggaagtaaat ccagcaatgc cagttggcct ccagaattcc
     5341 aaccaggagt gccatggaaa ggtatccaaa acattgaccc tgaatctgac ccctatgtca
     5401 ccccaggaag tgtgctgggg ggtacagcca catctcccat tgtagatact gaccaccaac
     5461 tgctgcggga taacaccaca gggtctaatt cttccctcaa cacctcgctg ccttcacctg
     5521 gtgcctggcc ctacagtgcc tctgacaact cctttaccaa cgttcatagc acttcagcaa
     5581 agttccctga ttacaaatca acatggtccc cagatcccat aggacacaac cccactcatc
     5641 tctccaacaa gatgtggaaa aaccatattt cctccaggaa cactacaccg ctgccccgcc
     5701 cacctcctgg tctgaccaac cccaaaccat catctccctg gagcagcaca gcaccccgat
     5761 cagtcagggg gtgggggaca caggactcac ggctcgcctc ggcctctacc tggagtgatg
     5821 gtggctcagt tcgtcctagt tactggctgg ttcttcacaa tctcacccca cagattgatg
     5881 ggtcaacctt gagaacgatc tgcatgcagc atggcccact gctgacattc catctgaatc
     5941 taacccaggg cactgccctg atccgataca gcaccaaaca ggaggcggcc aaggcccaaa
     6001 ctgcactgca catgtgtgtg ttgggaaaca ctaccatcct tgctgagttt gccactgatg
     6061 atgaagtcag ccgctttctg gcatgagcgg ccgcttccct ttagtgaggg ttaatgcttc
     6121 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6181 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6241 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     6301 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc cgataaggat
     6361 cgatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6421 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6481 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6541 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6601 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6661 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6721 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6781 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6841 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     6901 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
     6961 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     7021 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7081 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7201 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7261 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7321 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc
     7381 ttctgacaca acagtctcga acttaaggct agagccacca tgattgaaca agatggattg
     7441 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     7501 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     7561 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     7621 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     7681 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     7741 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7801 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7861 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7921 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7981 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8041 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8101 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     8161 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     8221 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga tggccgcaat
     8281 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagcgata
     8341 aggatccgcg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8401 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     8461 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     8521 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8581 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8641 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8701 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8761 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8821 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8881 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8941 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9001 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9061 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9121 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9181 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9241 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9301 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9901 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10381 cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatct
//
