LOCUS       sequence-288909-        8141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat_57-968-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-HA-DmHPat_57-968-dsRNAres_H
FEATURES             Location/Qualifiers
     source          1..8141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2594..2620
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5426..5467
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5477..5494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5517..5534)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5567..5701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5591..5610)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5645..5664
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6323..6911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6403..6422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7082..7873,7874..7942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7705..7724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7943..8047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8115..8133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gcttacccat acgatgttcc agattacgct cctccaagct tgaattctgc
     2641 agatatcatg ggcgaaaggg tgcggaagcg acccgcggaa tctagtggaa atacggattt
     2701 tgccgatcag ggaaatggtg ccttcctgcg acacgctaat ccgctatcat cggcgccctc
     2761 ttcaagcggc tccactccgg cccctttgaa tgcccccttc cgacagtctc gtcacatagg
     2821 ggactcggac ttggagctaa acatttcctc catgaagttg gacgacatgg acctgtcctc
     2881 tttcaccact gatagcgaag ccggtgggct gagcaaccgc attaagctgg actccggggt
     2941 ctggggatca cagccgttcc ccaacaacca acatttgttc cgcgagccta tgcgcagcgc
     3001 cttcaagcca cagcagcagc aacatcagca gcaccaacca caacaaatga agccaccgca
     3061 ggaagcgaat gccaactttg cactgaatcc acagcctttt ccaaaaatta caactctcga
     3121 ggatatagag cgcaatatga tcattcagca ggccattccg aaacaacagc agcagagcca
     3181 gcaacaggcg gaacgcaaaa tgtttgatga ttttagtctg ggtaacaggc agcaggtgcc
     3241 tacaccaacc atgttgcagc aacagatgtt gcagcagcag catcatcaga aacaacagtc
     3301 gcagcagcag ccgccacaac aagcgcagca caaggtaccc cctggcttct tgggcacgcc
     3361 gcacacctct ccaccgaggc caaatttggg tttccccgga cccgagctac tgcccacgcc
     3421 atcgtcgcag caacaactaa acggtattgg tggcaaccga gtgccgcccg gatttattta
     3481 cccgcaaggt ctgcccggga atattccgca attgccccag catcctctaa tgcagcagca
     3541 catgccgcac aactttccac tgcccggcgg caaccagcga cctctaccca atccacaagc
     3601 tctgcccacg gccctgaaca atttcgcgat gcatccaagc ttcaatgcga tgcgcgccgc
     3661 tggaatccat ccaggggctt ttatgcaacc tccccatccg caccagatgc tgccaccgcg
     3721 aatgccgccg cacccgctgc aagctgccac aagtctatta ggtcagcagc caccgaattc
     3781 catgtataac atgttcaaca tgcgactagt gcaagagatc cagcaaaacc atccgctgct
     3841 tcaacaggct gctgttgtac gccaaatgca acagagcaga gcaggatccg tggccagcga
     3901 tcgtcagaga tcgcaggcgc aacagcagca acaaggacga cgtccagatg gtacaggaaa
     3961 ttacccgctg gaagagtacg atgaatatgc caacctaatg agcacgcgcg acaagcactg
     4021 gctaatcgga atccagcttt cgcagctgaa tacggacacg ccgtatatcg acgactatta
     4081 ctacacggtg ttcagggagc gcaaggccca acagaacggg caaatgcgca acagtcaggc
     4141 gcacaaggac aaccagttga atcatccgct tactcagcca cgcggccatg cgcagcttat
     4201 tctcgtacag ttgggcaaca agaatggtac tcgcaatgga cacggacgtg agcgacgcaa
     4261 ctcggagaac accaacagca cgtcaggcaa cacgaacaat ggatcaggtg gtaacaacaa
     4321 caacctgaca ggctatatat tctcaccact gaagttcgag aactcactgg gaaagctaca
     4381 gtacggaagt gtgacagctc cgcgaaaaat tatcgatgct gacattatga gtggtgaatc
     4441 aaatgccgct gctgaagcca acgcaacttc ttcatcaaaa agctcgactc cgctgcccac
     4501 ctccgcgagc catgatgtaa atcccagtag catgcggaag tcgcgacaca ttttgcttcc
     4561 gattgaaaca ctgtatcgct atctgctgaa gttggaggat ttggaaagcc cagaagtgat
     4621 ggccaccatt gagctgaaaa agaaaaagga agccgaacgg atcgcagccc ttgagcagct
     4681 ggaaatggcc aataaaacgc ccgaagaacg ggcagcagag gcggctaacc cacagactat
     4741 gaacccacag ctcaaaaata aatttaatta cgaagttgaa actaaggcag cacttgtaaa
     4801 caagctaaaa gcgggacttg cttttgataa agtgattgct atgatgaacg tcagaaaagg
     4861 taaaattctg atccgccgta ttatgccgtt catagccgac cagtcgatac gatgggtggt
     4921 ctggtccgga gttttttgca gtctccagac tgttgtcaag aaagataaag atgatgtcga
     4981 gggagttctt tatgcactgt atccggaatt caagaagcat gtgaataagg caacatttga
     5041 gttactcgtc tcgatatctt cagcaatcac tcttaacgat aaaaagctgg caggcatctt
     5101 ctgttcccga ttcggcattc tgagccttgt ggctcttatt ctgagggctg aagagattta
     5161 tgttagccgc accgatgcca cgctgtcgga agaaaatcgc cagaaatgga gggagttcct
     5221 tgcccaagtg gccagctgtt taaaccggac catacagaat caaactatta gcgccgctat
     5281 cgaaagcgac gccatccaac ccttgatgga tcatttcgag cgcttcaagg acttgaagct
     5341 tgacgcctta ctagccctaa ttacggaggc tcgccaccag atcgactagg gcggccgctc
     5401 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     5461 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     5521 gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat
     5581 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     5641 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5701 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     5761 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc
     5821 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     5881 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     5941 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     6001 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     6061 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     6121 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     6181 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     6241 agggacagct tcaaggatcg ctcgcggctc ttaccagcca gcaaaaggcc aggaaccgta
     6301 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6361 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6421 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6481 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     6541 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6601 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6661 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6721 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6781 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6841 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6901 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6961 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7021 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7081 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7141 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7201 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7261 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7321 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7381 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7441 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7501 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7561 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7621 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7681 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7741 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7801 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7861 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7921 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7981 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8041 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     8101 cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
