LOCUS       sequence-288912-        8306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-HA-DmHPat-dsRNAres_H
FEATURES             Location/Qualifiers
     source          1..8306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2594..2620
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5591..5632
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5642..5659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5682..5699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5732..5866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5756..5775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5810..5829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6488..7076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6568..6587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7247..8038,8039..8107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7870..7889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8108..8212)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8280..8298
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gcttacccat acgatgttcc agattacgct cctccaagct tgaattctgc
     2641 agatatcatg gatgactcgt ttttcggctt tgacacgaat ctaccggacg aagatggtgg
     2701 cgatggacgc atcgtcgagg cggaatacga tgccctcaat gacgaaacct ttggatccgc
     2761 tataaatggc gactgggagg aagcccatga gacgatggta cgcctgggcg ggaatggcga
     2821 aagggtgcgg aagcgacccg cggaatctag tggaaatacg gattttgccg atcagggaaa
     2881 tggtgccttc ctgcgacacg ctaatccgct atcatcggcg ccctcttcaa gcggctccac
     2941 tccggcccct ttgaatgccc ccttccgaca gtctcgtcac ataggggact cggacttgga
     3001 gctaaacatt tcctccatga agttggacga catggacctg tcctctttca ccactgatag
     3061 cgaagccggt gggctgagca accgcattaa gctggactcc ggggtctggg gatcacagcc
     3121 gttccccaac aaccaacatt tgttccgcga gcctatgcgc agcgccttca agccacagca
     3181 gcagcaacat cagcagcacc aaccacaaca aatgaagcca ccgcaggaag cgaatgccaa
     3241 ctttgcactg aatccacagc cttttccaaa aattacaact ctcgaggata tagagcgcaa
     3301 tatgatcatt cagcaggcca ttccgaaaca acagcagcag agccagcaac aggcggaacg
     3361 caaaatgttt gatgatttta gtctgggtaa caggcagcag gtgcctacac caaccatgtt
     3421 gcagcaacag atgttgcagc agcagcatca tcagaaacaa cagtcgcagc agcagccgcc
     3481 acaacaagcg cagcacaagg taccccctgg cttcttgggc acgccgcaca cctctccacc
     3541 gaggccaaat ttgggtttcc ccggacccga gctactgccc acgccatcgt cgcagcaaca
     3601 actaaacggt attggtggca accgagtgcc gcccggattt atttacccgc aaggtctgcc
     3661 cgggaatatt ccgcaattgc cccagcatcc tctaatgcag cagcacatgc cgcacaactt
     3721 tccactgccc ggcggcaacc agcgacctct acccaatcca caagctctgc ccacggccct
     3781 gaacaatttc gcgatgcatc caagcttcaa tgcgatgcgc gccgctggaa tccatccagg
     3841 ggcttttatg caacctcccc atccgcacca gatgctgcca ccgcgaatgc cgccgcaccc
     3901 gctgcaagct gccacaagtc tattaggcca gcagccaccg aattccatgt ataacatgtt
     3961 caacatgcga ctagtgcaag agatccagca aaaccatccg ctgcttcaac aggctgctgt
     4021 tgtacgccaa atgcaacaga gcagagcagg atccgtggcc agcgatcgtc agagatcgca
     4081 ggcgcaacag cagcaacaag gacgacgtcc agatggtaca ggaaattacc cgctggaaga
     4141 gtacgatgaa tatgccaacc taatgagcac gcgcgacaag cactggctaa tcggaatcca
     4201 gctttcgcag ctgaatacgg acacgccgta tatcgacgac tattactaca cggtgttcag
     4261 ggagcgcaag gcccaacaga acgggcaaat gcgcaacagt caggcgcaca aggacaacca
     4321 gttgaatcat ccgcttactc agccacgcgg ccatgcgcag cttattctcg tacagttggg
     4381 caacaagaat ggtactcgca atggacacgg acgtgagcga cgcaactcgg agaacaccaa
     4441 cagcacgtca ggcaacacga acaatggatc aggtggtaac aacaacaacc tgacaggcta
     4501 tatattctca ccactgaagt tcgagaactc actgggaaag ctacagtacg gaagtgtgac
     4561 agctccgcga aaaattatcg atgctgacat tatgagtggt gaatcaaatg ccgctgctga
     4621 agccaacgca acttcttcat caaaaagctc gactccgctg cccacctccg cgagccatga
     4681 tgtaaatccc agtagcatgc ggaagtcgcg acacattttg cttctgattg aaacactgta
     4741 tcgctatctg ctgaagttgg aggatttgga aagcccagaa gtgatggcca ccattgagct
     4801 gaaaaagaaa aaggaagccg aacggatcgc agcccttgag cagctggaaa tggccaataa
     4861 aacgcccgaa gaacgggcag cagaggcggc taacccacag actatgaacc cacagctcaa
     4921 aaataaattt aattacgaag ttgaaactaa ggcagcactt gtaaacaagc taaaagcggg
     4981 acttgctttt gataaagtga ttgctatgat gaacgtcaga aaaggtaaaa ttctgatccg
     5041 ccgtattatg ccgttcatag ccgaccagtc gatacgatgg gtggtctggt ccggagtttt
     5101 ttgcagtctc cagactgttg tcaagaaaga taaagatgat gtcgagggag ttctttatgc
     5161 actgtatccg gaattcaaga agcatgtgaa taaggcaaca tttgagttac tcgtctcgat
     5221 atcttcagca atcactctta acgataaaaa gctggcaggc atcttctgtt cccgattcgg
     5281 cattctgagc cttgtggctc ttattctgag ggctgaagag atttatgtta gccgcaccga
     5341 tgccacgctg tcggaagaaa atcgccagaa atggagggag ttccttgccc aagtggccag
     5401 ctgtttaaac cggaccatac agaatcaaac tattagcgcc gctatcgaaa gcgacgccat
     5461 ccaacccttg atggatcatt tcgagcgctt caaggacttg aagcttgacg ccttactagc
     5521 cctaattacg gaggctcgcc accagatcga ctagggcggc cgctcgagtc tagagggccc
     5581 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     5641 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     5701 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     5761 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5821 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5881 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5941 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     6001 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     6061 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     6121 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     6181 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     6241 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     6301 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     6361 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     6421 gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6481 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6541 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6601 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6661 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     6721 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6781 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6841 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6901 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6961 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7021 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7081 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7141 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7201 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7261 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7321 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7381 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7441 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7501 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7561 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7621 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7681 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7741 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7801 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7861 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7921 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7981 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8041 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8101 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8161 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8221 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8281 ataggcgtat cacgaggccc tttcgt
//
