LOCUS       sequence-288914-        8225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat_57-968.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmHPat_57-968_H
FEATURES             Location/Qualifiers
     source          1..8225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5510..5551
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5561..5578
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5601..5618)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5651..5785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5675..5694)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5729..5748
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6407..6995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6487..6506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7166..7957,7958..8026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7789..7808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8027..8131)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8199..8217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgggcgaa agggtgcgga agcgacccgc
     2761 ggaatctagt ggaaatacgg attttgccga tcagggaaat ggtgccttcc tgcgacacgc
     2821 taatccgcta tcatcggcgc cctcttcaag cggctccact ccggcccctt tgaatgcccc
     2881 cttccgacag tctcgtcaca taggggactc ggacttggag ctaaacattt cctccatgaa
     2941 gttggacgac atggacctgt cctctttcac cactgatagc gaagccggtg ggctgagcaa
     3001 ccgcattaag ctggactccg gggtctgggg atcacagccg ttccccaaca accaacattt
     3061 gttccgcgag cctatgcgca gcgccttcaa gccacagcag cagcaacatc agcagcacca
     3121 accacaacaa atgaagccac cgcaggaagc gaatgccaac tttgcactga atccacagcc
     3181 ttttccaaaa attacaactc tcgaggatat agagcgcaat atgatcattc agcaggccat
     3241 tccgaaacaa cagcagcaga gccagcaaca ggcggaacgc aaaatgtttg atgattttag
     3301 tctgggtaac aggcagcagg tgcctacacc aaccatgttg cagcaacaga tgttgcagca
     3361 gcagcatcat cagaaacaac agtcgcagca gcagccgcca caacaagcgc agcacaaggt
     3421 accccctggc ttcttgggca cgccgcacac ctctccaccg aggccaaatt tgggtttccc
     3481 cggacccgag ctactgccca cgccatcgtc gcagcaacaa ctaaacggta ttggtggcaa
     3541 ccgagtgccg cccggattta tttacccgca aggtctgccc gggaatattc cgcaattgcc
     3601 ccagcatcct ctaatgcagc agcacatgcc gcacaacttt ccactgcccg gcggcaacca
     3661 gcgacctcta cccaatccac aagctctgcc cacggccctg aacaatttcg cgatgcatcc
     3721 aagcttcaat gcgatgcgcg ccgctggaat ccatccaggg gcttttatgc aacctcccca
     3781 tccgcaccag atgctgccac cgcgaatgcc gccgcacccg ctgcaagctg ccacaagtct
     3841 attaggccag cagccaccga attccatgta taacatgttc aacatgcgac tagtgcaaga
     3901 gatccagcaa aaccatccgc tgcttcaaca ggctgctgtt gtacgccaaa tgcaacagag
     3961 cagagcagga tccgtggcca gcgatcgtca gagatcgcag gcgcaacagc agcaacaagg
     4021 acgacgtcca gatggtacag gaaattaccc gctggaagag tacgatgaat atgccaacct
     4081 aatgagcacg cgcgacaagc actggctaat cggaatccag ctttcgcagc tgaatacgga
     4141 cacgccgtat atcgacgact attactacac ggtgttcagg gagcgcaagg cccaacagaa
     4201 cgggcaaatg cgcaacagtc aggcgcacaa ggacaaccag ttgaatcatc cgcttactca
     4261 gccacgcggc catgcgcagc ttattctcgt acagttgggc aacaagaatg gtactcgcaa
     4321 tggacacgga cgtgagcgac gcaactcgga gaacaccaac agcacgtcag gcaacacgaa
     4381 caatggatca ggtggtaaca acaacaacct gacaggctat atattctcac cactgaagtt
     4441 cgagaactca ctgggaaagc tacagtacgg aagtgtgaca gctccgcgaa aaattatcga
     4501 tgctgacatt atgagtggtg aatcaaatgc cgctgctgaa gccaacgcaa cttcttcatc
     4561 aaaaagctcg actccgctgc ccacctccgc gagccatgat gtaaatccca gtagcatgcg
     4621 gaagtcgcga cacattttgc ttctgattga aacactgtat cgctatctgc tgaagttgga
     4681 ggatttggaa agcccagaag tgatggccac cattgagctg aaaaagaaaa aggaagccga
     4741 acggatcgca gcccttgagc agctggaaat ggccaataaa acgcccgaag aacgtgctgc
     4801 tgaagccgcc aatccgcaaa ccatgaatcc gcaactaaag aacaagttca actatgaggt
     4861 agagacgaaa gctgcgttgg tcaataaatt aaaggcaggt ctggccttcg acaaggttat
     4921 cgccatgatg aatgtgcgca agggaaagat acttattaga cgcataatgc cctttattgc
     4981 ggatcaaagc attcgctggg tcgtgtggag cggcgtcttc tgttccctac aaacagtggt
     5041 gaaaaaggac aaggacgacg ttgaaggcgt cttgtacgct ctttacccag agtttaaaaa
     5101 acacgttaac aaagctactt tcgaactcct agtgagcatt tcatctgcta ttacgctgaa
     5161 tgacaagaaa cttgctggaa ttttttgcag tcgctttgga atcctttcgc tggttgccct
     5221 gatacttcgc gccgaggaaa tctacgtctc gcgaactgac gcaactctta gcgaggagaa
     5281 caggcaaaag tggcgagaat ttctggcaca ggttgcatcc tgcctcaatc gcactattca
     5341 aaaccagacc atctcggcag ccattgagtc ggatgcaatt cagccactta tggaccactt
     5401 tgaacggttt aaagatctta aactggatgc actcttagca ttaataactg aagccaggca
     5461 tcaaattgat tagggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5521 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     5581 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     5641 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     5701 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5761 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5821 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     5881 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     5941 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     6001 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     6061 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     6121 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     6181 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     6241 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     6301 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     6361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6601 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6841 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     7501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     7801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8161 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8221 ttcgt
//
