LOCUS       sequence-288918-        7835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat-del500-684.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmHPat-del500-684_H
FEATURES             Location/Qualifiers
     source          1..7835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5120..5161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5171..5188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5211..5228)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5261..5395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5285..5304)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5339..5358
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6017..6605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6097..6116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6776..7567,7568..7636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7399..7418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7637..7741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7809..7827
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggatgac tcgtttttcg gctttgacac
     2761 gaatctaccg gacgaagatg gtggcgatgg acgcatcgtc gaggcggaat acgatgccct
     2821 caatgacgaa acctttggat ccgctataaa tggcgactgg gaggaagccc atgagacgat
     2881 ggtacgcctg ggcgggaatg gcgaaagggt gcggaagcga cccgcggaat ctagtggaaa
     2941 tacggatttt gccgatcagg gaaatggtgc cttcctgcga cacgctaatc cgctatcatc
     3001 ggcgccctct tcaagcggct ccactccggc ccctttgaat gcccccttcc gacagtctcg
     3061 tcacataggg gactcggact tggagctaaa catttcctcc atgaagttgg acgacatgga
     3121 cctgtcctct ttcaccactg atagcgaagc cggtgggctg agcaaccgca ttaagctgga
     3181 ctccggggtc tggggatcac agccgttccc caacaaccaa catttgttcc gcgagcctat
     3241 gcgcagcgcc ttcaagccac agcagcagca acatcagcag caccaaccac aacaaatgaa
     3301 gccaccgcag gaagcgaatg ccaactttgc actgaatcca cagccttttc caaaaattac
     3361 aactctcgag gatatagagc gcaatatgat cattcagcag gccattccga aacaacagca
     3421 gcagagccag caacaggcgg aacgcaaaat gtttgatgat tttagtctgg gtaacaggca
     3481 gcaggtgcct acaccaacca tgttgcagca acagatgttg cagcagcagc atcatcagaa
     3541 acaacagtcg cagcagcagc cgccacaaca agcgcagcac aaggtacccc ctggcttctt
     3601 gggcacgccg cacacctctc caccgaggcc aaatttgggt ttccccggac ccgagctact
     3661 gcccacgcca tcgtcgcagc aacaactaaa cggtattggt ggcaaccgag tgccgcccgg
     3721 atttatttac ccgcaaggtc tgcccgggaa tattccgcaa ttgccccagc atcctctaat
     3781 gcagcagcac atgccgcaca actttccact gcccggcggc aaccagcgac ctctacccaa
     3841 tccacaagct ctgcccacgg ccctgaacaa tttcgcgatg catccaagct tcaatgcgat
     3901 gcgcgccgct ggaatccatc caggggcttt tatgcaacct ccccatccgc accagatgct
     3961 gccaccgcga atgccgccgc acccgctgca agctgccaca agtctattag gccagcagcc
     4021 accgaattcc atgtataaca tgttcaacat gcgactagtg caagagatcc agcaaaacca
     4081 tccgctgctt caacaggctg ctgttgtacg ccaaatgcaa cagagcagag caggatccgt
     4141 ggccagcgat cgtcaaagat cgcaggcgca acagcagcaa caaggacgac gtccagatgg
     4201 tacaggaaat tacccgctgg aagagtaccg gaagtcgcga cacattttgc ttctgattga
     4261 aacactgtat cgctatctgc tgaagttgga ggatttggaa agcccagaag tgatggccac
     4321 cattgagctg aaaaagaaaa aggaagccga acggatcgca gcccttgagc agctggaaat
     4381 ggccaataaa acgcccgaag aacgtgctgc tgaagccgcc aatccgcaaa ccatgaatcc
     4441 gcaactaaag aacaagttca actatgaggt agagacgaaa gctgcgttgg tcaataaatt
     4501 aaaggcaggt ctggccttcg acaaggttat cgccatgatg aatgtgcgca agggaaagat
     4561 acttattaga cgcataatgc cctttattgc ggatcaaagc attcgctggg tcgtgtggag
     4621 cggcgtcttc tgttccctac aaacagtggt gaaaaaggac aaggacgacg ttgaaggcgt
     4681 cttgtacgct ctttacccag agtttaaaaa acacgttaac aaagctactt tcgaactcct
     4741 agtgagcatt tcatctgcta ttacgctgaa tgacaagaaa cttgctggaa ttttttgcag
     4801 tcgctttgga atcctttcgc tggttgccct gatacttcgc gccgaggaaa tctacgtctc
     4861 gcgaactgac gcaactctta gcgaggagaa caggcaaaag tggcgagaat ttctggcaca
     4921 ggttgcatcc tgcctcaatc gcactattca aaaccagacc atctcggcag ccattgagtc
     4981 ggatgcaatt cagccactta tggaccactt tgaacggttt aaagatctta aactggatgc
     5041 actcttagca ttaataactg aagccaggca tcaaattgat tagggcggcc gctcgagtct
     5101 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5161 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     5221 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     5281 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5341 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5401 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     5461 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga
     5521 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     5581 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     5641 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     5701 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     5761 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     5821 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     5881 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     5941 agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg
     6001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6181 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     6241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6421 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7081 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7381 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7741 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     7801 cctataaaaa taggcgtatc acgaggccct ttcgt
//
