LOCUS       sequence-28893-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Fuentealba LC, Eivers E, Ikeda A, Hurtado C, Kuroda H, Pera EM, De 
            Robertis EM
  TITLE     Integrating patterning signals: Wnt/GSK3 regulates the duration of 
            the BMP/Smad1 signal.
  JOURNAL   Cell. 2007 Nov 30. 131(5):980-93.
  PUBMED    18045539
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):980-93."
COMMENT     Sequence Label: pCS2 hSmad1-GM
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             115..138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atctggtacc acgcgtatcg ataagcttac catggactac
      121 aaggacgacg atgacaagaa tgtgacaagt ttattttcct ttacaagtcc agctgtgaag
      181 agacttcttg ggtggaaaca gggcgatgaa gaagaaaaat gggcagagaa agctgttgat
      241 gctttggtga aaaaactgaa gaaaaagaaa ggtgccatgg aggaactgga aaaggccttg
      301 agctgcccag ggcaaccgag taactgtgtc accattcccc gctctctgga tggcaggctg
      361 caagtctccc accggaaggg actgcctcat gtcatttact gccgtgtgtg gcgctggccc
      421 gatcttcaga gccaccatga actaaaacca ctggaatgct gtgagtttcc ttttggttcc
      481 aagcagaagg aggtctgcat caatccctac cactataaga gagtagaaag ccctgtactt
      541 cctcctgtgc tggttccaag acacagcgaa tataatcctc agcacagcct cttagctcag
      601 ttccgtaact taggacaaaa tgagcctcac atgccactca acgccacttt tc
//
