LOCUS       sequence-28894-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Fuentealba LC, Eivers E, Ikeda A, Hurtado C, Kuroda H, Pera EM, De 
            Robertis EM
  TITLE     Integrating patterning signals: Wnt/GSK3 regulates the duration of 
            the BMP/Smad1 signal.
  JOURNAL   Cell. 2007 Nov 30. 131(5):980-93.
  PUBMED    18045539
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):980-93."
COMMENT     Sequence Label: pCS2 hSmad1-EVE
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    393..527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(417..436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     471..490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(640..660)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(640..658)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcggactgca actaccatca tggatttcat cctactactg tttgcaagat ccctagtggg
       61 tgtagtctga aaatttttaa caaccaagaa tttgctcagt tattggcaca gtctgtgaac
      121 catggatttg agacagtcta tgagcttaca aaaatgtgta ctatacgtat gagctttgtg
      181 aagggctggg gagcagaata ccaccgccag gatgttacta gcaccccctg ctggattgag
      241 atacatctgc acggccccct ccagtggctg gataaagttc ttactcaaat gggttcacct
      301 cataatccta tttcagaagt agaataagga tcccatcgat tcgaattcaa ggcctctcga
      361 gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
      421 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      481 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      541 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggntttttaa ttcgcggccg
      601 cggcgncnat gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
      661 gcgcttggcg taatcatggt catagct
//
