LOCUS       sequence-288957-        9379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C_1-1254.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C_1-1254_H
FEATURES             Location/Qualifiers
     source          1..9379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3554..3571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5083..5204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5094..5113)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5148..5167
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5390..5845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5477..5496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5687..5708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5962..6319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5962..6153
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5985..6005)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6170..6305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6370..7164
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6424..6443)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7034..7053
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7228..7276
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7317..7336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7436..7458
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7496..7514)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7582..7686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7687..7755,7756..8547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7905..7924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8718..9306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9207..9226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc acgcgtggta
     1261 cctctagagt cgacatggct acagggagtg cccagggcaa cttcactgga cataccaaga
     1321 agacaaatgg caataatggc accaatggcg cactcgtcca aagcccttct aatcagagtg
     1381 cccttggagc agggggagcg aacagtaatg gaagtgcggc cagagtgtgg ggtgtagcca
     1441 caggctccag ctctggcctg gctcactgct ctgtcagtgg tggggatgga aaaatggaca
     1501 ctatgattgg agatgggaga agtcagaatt gctggggtgc ttccaactcc aatgctggca
     1561 ttaatcttaa ccttaatcct aatgccaacc cagctgcctg gcctgtactt ggacatgaag
     1621 gaaccgtggc gacaggcaac ccttccagta tttgcagtcc agtcagtgcc ataggtcaaa
     1681 atatgggcaa ccagaacggg aacccaacag gcactttagg tgcttgggga aacttgctgc
     1741 cacaagagag cacagaacca caaacgtcca cttctcagaa tgtgtctttc agcgcacaac
     1801 ctcagaacct taacactgat ggaccaaata acactaaccc catgaactct tcacccaacc
     1861 ctatcaatgc aatgcagaca aatggactgc caaactgggg catggctgtt ggtatggggg
     1921 ccatcatccc gccccacctg caaggccttc ctggtgctaa tggatcatca gtttctcaag
     1981 tcagtggggg cagtgctgaa ggaataagca attctgtgtg gggactgtcc ccaggtaacc
     2041 ctgccacagg aaatagcaat tctgggttca gtcaggggaa tggagacact gtgaactcag
     2101 cattaagtgc taaacaaaat ggatccagca gtgctgtgca aaaggaagga agtggaggaa
     2161 atgcttggga ttcaggacct cctgctggtc ctggaatact cgcctgggga aggggcagtg
     2221 gcaacaatgg cgttggtaat atccattcag gagcttgggg ccaccccagc cgaagcacct
     2281 ctaacggtgt gaatggggaa tggggaaagc ccccaaacca gcattccaac agtgacatca
     2341 atgggaaagg atcaacaggg tgggagagtc ctagtgtcac cagccagaac cctaccgtac
     2401 agcctggtgg tgaacacatg aactcctggg ccaaagcggc atcttctgga actacagcaa
     2461 gtgaaggaag tagtgatggt tctggcaacc acaatgaagg aagcactggg agggaaggaa
     2521 cgggagaagg ccgaaggcga gataaaggga ttatagacca agggcacatc cagttgccaa
     2581 ggaatgatct tgacccaaga gttctgtcta atactggttg gggacagact cctgtaaagc
     2641 aaaacactgc ctgggaattt gaagaatccc ctaggtctga aaggaaaaat gacaatggga
     2701 cagaggcctg gggttgtgca gctactcagg cttcaaactc aggggggaag aacgatgggt
     2761 ccatcatgaa cagtacaaat acctcttcag tatctgggtg ggtcaacgcg ccacctgccg
     2821 ctgtgccagc aaacacaggt tggggagaca gcaacaacaa agcgccaagt ggcccggggg
     2881 tttgggggga ctcgataagc tctactgctg ttagtactgc tgctgctgcc aagagtggcc
     2941 atgcttggag tggggccgca aatcaggagg acaagtcacc cacctggggt gagcctccaa
     3001 agcccaaatc ccaacactgg ggagatggac aaagatcaaa tccagcctgg agtgcaggag
     3061 ggggagattg ggcagattca tcgtctgtcc ttggacactt gggggatggg aaaaaaaatg
     3121 gatctggatg ggatgctgac agtaataggt cagggtctgg ttggaatgac accacgagat
     3181 ctgggaacag tggctggggc aacagcacaa atacaaaggc caatccaggt acaaactggg
     3241 gggagacttt aaaacctggc ccccaacaga actgggctag caaaccccaa gacaacaatg
     3301 tgagtaactg gggaggagct gcttctgtga aacagacagg aacagggtgg atcggggggc
     3361 cggtaccggt caaacagaag gacagcagtg aagcaactgg ctgggaagaa ccctctccac
     3421 cgtccattcg ccgcaaaatg gaaattgatg atggtacctc agcttggggg gacccaagca
     3481 actataacaa taaaactgta aacatgtggg atagaaacaa cccggtcatc cagagcagta
     3541 ccacgaccaa taccaccacc accaccacca ctaccacgag caacaccaca cacagggtcg
     3601 agacgccgcc cccgcaccag gctggtactc agctgaatcg atcaccgttg cttggtccag
     3661 gtaggaaagt ttcatcaggc tggggagaaa tgcctaatgt tcactcaaag actgaaaact
     3721 cttggggaga accatcctcc ccttctaccc tggtggataa tggcacagca gcatggggga
     3781 agccacccag cagtggcagc gggtggggag atcaccctgc cgagccgccg gtggcatttg
     3841 gaagagctgg cgcacctgtt gctgcctcag ccctgtgcaa accagcttca aaatctatgc
     3901 aagaaggctg gggcagtggt ggggatgaaa tgaacctcag taccagccag tgggaggatg
     3961 aagaagggga cgtgtggaat aatgctgctt cccaagaaag cacctcctcc tgcagctcct
     4021 gggggaacgc ccccaaaaaa ggacttcaaa agggcatgaa gacgtctggc aagcaggatg
     4081 aggcctggat catgagccgg ctgatcaaac aactcacaga catgggcttc ccgagagagc
     4141 cagctgagga ggccttgaag agtaacaata tgaatcttga tcaggccatg agcgctctgc
     4201 tggaaaagaa ggtggacgtg gacaagcgtg ggctgggagt gaccgaccat aatggaatgg
     4261 ccgccaagcc cctcggctgc cgcccgccaa tctccaaaga gtcttccgtg gaccgcccca
     4321 cctttcttga caaggatggc ggcctcgtgg aagagcccac gccttcaccg ttcttgcctt
     4381 ccccaagcct gaagctcccc ctttcacaca gtgcactccc cagtcaggcc ctgggtggga
     4441 ttgcctccgg gctgggcatg caaaacttga attcttctag acagataccg agtggcaatc
     4501 tgggtatgtt tggcaatagt ggagcagcac aagccaggac catgcagcag ccgccacagc
     4561 caccagtgca gcctcttaac tcttcccagc ccagtctccg tgctcaagtg cctcagtttc
     4621 tatcccctca ggttcaagca cagcttttgc agtttgcagc aaaaaacatt ggtctcaacc
     4681 ctgcactatt aacctcgcca attaatcctc aacatatgac gatgttgaac cagctctatc
     4741 agctgcagct ggcataccaa cgtttacaaa tccagcagca gatgttacag gcccagcgta
     4801 atgtgtccgg atccatgaga caacaggagc agcaagttgc gcgcacaatc actaatctgc
     4861 agcagcagat ccagcagcac cagcgccagc tggcccaggc cctgctcgtg aagcagccac
     4921 caccgccacc gcccccgccg cacctgtctc tgcacccctc tgcaggcaaa tcggccatgg
     4981 acagcttccc ctcgcaccca cagactcccg gcctacctga cctgcagacc aaagaggcgg
     5041 ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac attgatgagt
     5101 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     5161 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     5221 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     5281 tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata gcgaagaggc
     5341 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     5401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5761 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5821 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     5881 gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag
     5941 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     6001 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6061 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6121 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6181 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6241 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6301 aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga acttaaggct
     6361 agagccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6421 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6481 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6541 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6601 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6661 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6721 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6781 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6841 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6901 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6961 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     7021 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     7081 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7141 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     7201 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     7261 gttggttttt tgtgtgaatc gatagcgata aggatccgcg tatggtgcac tctcagtaca
     7321 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7381 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7441 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     7501 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7621 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7681 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7741 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7801 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7861 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7921 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7981 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8041 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8101 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8161 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8221 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8281 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8341 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8401 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8461 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8521 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8581 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8641 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8761 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8881 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8941 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9001 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9061 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9121 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9181 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9241 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9301 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9361 cacatggctc gacagatct
//
