LOCUS       sequence-288968-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delCterm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delCterm-V5His6_H
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6368..6409
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6419..6436
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6459..6476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6509..6643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6533..6552)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6587..6606
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7265..7853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7345..7364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8024..8815,8816..8884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8647..8666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8885..8989)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9057..9075
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcgtgaagc
     2761 ccttttttcc caagatggct ggggctgtca gcatgttaac caggatacta attgggaagt
     2821 tcccagttcg ccagaaccag ccaataagga tgcacccggt ccaccaatgt ggaagccaag
     2881 cattaacaat ggtactgatc tttgggagtc caatttgaga aacggaggtc agccggccgc
     2941 acagcaagtt ccaaagccgt cgtggggtca tacaccatcc tctaacttag gtggaacatg
     3001 gggtgaggac gacgatggcg ccgatagtag tagtgtgtgg actggaggag ctgttagcaa
     3061 cgcgggatcc ggagctgcag tgggagtaaa ccaagccgga gttaatgtcg gtccaggcgg
     3121 tgttgtttcg tctggcggac ctcagtgggg acaaggtgtc gttggcgtcg gacttggatc
     3181 aactggaggt aacgggtcaa gcaatataac tggatcgtct ggagtcgcaa caggtagtag
     3241 cggaaactcc agcaacgctg gtaacggttg gggagaccct cgtgaaatac gccctttggg
     3301 agttggtggc tccatggata ttcgaaatgt tgaacatcgc ggcggtaacg gttctggagc
     3361 aacttcgagc gatccacgag acattcgcat gatcgatccg cgtgacccta ttcgaggaga
     3421 tccccgtgga atatctggtc gtcttaatgg gacctctgaa atgtggggtc atcatccaca
     3481 aatgtcccat aaccagttgc aaggtatcaa caaaatggtt ggtcaaagtg tagcaactgc
     3541 cagcaccagt gtcggaacat ctggctcggg catcggtcct ggaggtcccg gtcctagtac
     3601 agtatcaggc aatatcccaa cacagtgggg gcctgctcaa ccggtaagcg ttggtgtaag
     3661 tggtcccaaa gacatgtcaa aacagataag tggatgggag gaaccatcac caccgcctca
     3721 gcgtcgcagt attcctaact acgatgatgg tacatcgttg tggggtcagc aaactcgtgt
     3781 tcccgctgca agcggtcact ggaaagacat gactgattcg ataggtcgta gtagtcatct
     3841 catgcgtggc caaagccaaa cgggaggtat aggaatagcc ggcgttggaa atagcaatgt
     3901 tccagtggga gccaatccaa gtaatcctat aagcagtgta gttggacctc aagcccggat
     3961 tccatctgtg ggcggcgtac aacacaaacc agacggcggc gctatgtggg tgcattccgg
     4021 caatgtaggt ggcagaaata atgttgctgc tgttactact tggggagatg acactcatag
     4081 cgttaatgtc ggcgctccca gcagtggcag tgtatccagc aacaattggg ttgatgacaa
     4141 gtccaactca accttggcac aaaactcttg gagcgacccg gcccctgttg gagttagttg
     4201 gggcaataag caaagcaaac cgccaagcaa tagtgcttca tcaggttgga gcactgctgc
     4261 gggcgtggtg gatggggttg atctaggatc tgagtggaac acgcacgggg ggattattgg
     4321 aaaatctcag caacaacaaa aactagcggg acttaacgtg ggaatggtga acgtaattaa
     4381 cgcggagatc attaagcaaa gcaagcaata caggatcctt gtcgagaacg gctttaaaaa
     4441 ggaagatgta gagcgggcat tagtgattgc taatatgaac atcgaagagg cagccgatat
     4501 gctccgtgcc aactcatccc tatcaatgga tggttggcgt cgacatgatg agtcccttgg
     4561 atcttatgcc gaccacaata gttcaacaag cagcggtgga tttgctggtc gttacccggt
     4621 caacagtgga caaccttcaa tgtcctttcc tcataataac cttatgaata acatgggagg
     4681 taccgctgtt actggaggta acaacaatac aaacatgaca gctttacagg tgcaaaagta
     4741 tttaaatcaa gggcaacatg gtgtcgctgt tggaccgcaa gccgttggta attcttcagc
     4801 agtatctgtc ggatttggtc agaacacgtc taacgcagca gtggcaggag cagcctctgt
     4861 aaatatagca gcaaatacaa acaaccaacc gtctggtcag caaattcgca tgctaggcca
     4921 gcaaattcag ttggccattc atagtggttt catatctagt cagatattga ctcaaccgct
     4981 aactcaaaca acccttaacc ttttaaacca acttcttagc aatattaagc atctccaggc
     5041 tgcgcagcaa tcccttaccc gcgggggaaa tgtcaatcca atggcagtga atgtggctat
     5101 atctaaatac aagcagcaaa tccagaattt acagaaccag ataaatgcac aacaggctgt
     5161 gtatgtaaaa cagcaaaata tgcaaccaac ttcacaacaa caacagcccc aacaacagca
     5221 acttccttct gttcatctaa gtaactcagg caacgactat ttaagaggtc acgatgcaat
     5281 aaataatttg caaagcaact tttctgagct caatattaat aagccaagtg gatatcaagg
     5341 agcgtccaat caacaatccc gattaaatca gtggaagctt ccagtattag ataaggagat
     5401 caactctgac agtacggaat tttctcgtgc cccaggtgca acgaaacaaa atttgacggc
     5461 caacacaagc aacataaact ctttgggtct tcaaaacgat agtacatggt caactggacg
     5521 cagtattggt gacggttggc ctgatccctc atctgataac gagaataaag actggtctgt
     5581 tgctcagcca acttcagcag caactgctta cactgatctg gtccaagagt ttgagccagg
     5641 caagccatgg aagggttcac agatcaaaag catagaagat gatcccagca ttacaccagg
     5701 aagcgttgct agatctccat tgtctattaa ttcgacgcca aaagatgctg acatatttgc
     5761 caataccggt aaaaattcac cgactgattt accgccacta agtttatcgt cgtctacatg
     5821 gagttttaat ccaaaccaaa attatccgag tcacagttgg tctgacaata gtcaacaatg
     5881 taccgccact tcggagcttt ggacaagccc gctaaataaa tcatcgtctc gaggtccccc
     5941 gccaggattg actgccaatt caaataaatc tgcaaatagt aatgcgtcaa cgccaacaac
     6001 tattaccgga ggtgcgaatg gatggttaca gcctcgaagt ggcggtgttc aaaccacaaa
     6061 cactaattgg acaggtggta acaccacttg gggctccagt tggttgcttt tgaaaaatct
     6121 aacagcacag attgatggtc ctactttgcg tacactgtgt atgcagcatg ggccccttgt
     6181 cagctttcac ccgtatttga accaaggaat tgccttatgt aaatatacta ctcgtgagga
     6241 ggcgaacaag gcgcaaatgg cgttaaacaa ctgtgtcctc gccaacacca caatatttgc
     6301 tgaatctccc agcgagaacg aggtgcaaag cattatgcag cacttactag agggcccgcg
     6361 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     6421 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     6481 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     6541 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6601 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6661 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     6721 ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc
     6781 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     6841 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     6901 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     6961 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     7021 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     7081 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     7141 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     7201 cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7441 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     7501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7681 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     8341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8641 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9001 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     9061 ggcgtatcac gaggcccttt cgt
//
